ITT 4chan cures blindness

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ITT 4chan cures blindness Genetic Eng !neEr...dvM Sun Mar 25 05:11:29 2018 No.9618267 [Reply] [Original]

PREVIOUS THREAD: >>9554648

WIKI: https://github.com/EyesOpenCure/EyesOpenProject/wiki (barebones work in progress)

>WTF are you doing?
Protein replacement therapy for retinitis pigmentosa type 25 and also other retinal injuries. EYS is a protein that gets secreted by the retina to protect it from damage. It also belongs to a class of proteins that have some really crazy tissue regeneration properties. [1] We are going to grow it in cell cultures and then inject it, a lot like how diabetics get insulin injections.

>What is your plan?
We are going to start by testing in zebrafish. Some fluorescent tagged EYS is going to get injected into their eye and then we will see if it goes to the right place (retinal outer segment). If all goes well, we then modify some zebrafish so that they are missing EYS, and track the visual decline of treated vs. untreated fish. Visual function is measured by wrapping the fish in a wire electrode, showing them flashes of light, and then recording the electrical signals.

>Are you insane?
No, just extremely motivated.

[1] https://www.sciencedaily.com/releases/2017/06/170606095033.htm

>>	Genetic Eng !neEr...dvM Sun Mar 25 05:25:58 2018 No.9618287 Day 24: Had time to work a little bit more on the wiki. Some family members want to donate to the project as well. Right now I really, really need to set aside enough time to finish working on the whitepaper before this Tuesday.

Genetic Eng !neEr...dvM Sun Mar 25 05:31:00 2018 No.9618290

>>9616871
They aren't currently, but that's a really good idea. My progress on this has really slowed down this week because of being busy with outside stuff, but I'll work on adding that.

>>9616954
>>9617895
>EYS wide open
Damn, I should have used that.

>>9616960
>ITT 4chan cures blindness

>>	Anonymous Sun Mar 25 05:52:17 2018 No.9618304 >>9618267 >No, just extremely motivated. From my experience in Dwarf Fortress I can tell you that there's a very thin line between extreme motivation and insanity.

>>	Anonymous Sun Mar 25 05:59:13 2018 No.9618314 >>9618290 So you can't change the name of the project on github? Also, is the list of tasks in the project wiki completed as in thre won't be any new tasks added?

>>	Anonymous Sun Mar 25 05:59:48 2018 No.9618317 >>9618290 >Damn, I should have used that. change it now or make a new one name is important

>>	\|-x'\| Sun Mar 25 07:00:29 2018 No.9618373 >>9618304 The required resolution to identify said line is negligible. We can isolate any 'wave' but the sectional monoclusters left are relative and still nearest-k neighbors. Methmetically speaking, ya gotta be both if you want a chance of either.

>>	Anonymous Sun Mar 25 08:52:11 2018 No.9618505 >>9618267 How are you going to measure the electric signals in the zebrafish eyes?

>>	Anonymous Sun Mar 25 09:59:45 2018 No.9618576 >>9618267 >a lot like how diabetics get insulin injections. the hell is this? you realize insulin isn't injected into the pancreas, right?

>>	Anonymous Sun Mar 25 10:20:58 2018 No.9618595 >>9618576 He probably just meant regular injections.

>>	Anonymous Sun Mar 25 15:14:39 2018 No.9619124 >>9618267 Have you sent the project link to anons, who gave you their emails in the previous thread? Some of them might not have seen this one.

Anonymous Sun Mar 25 16:37:05 2018 No.9619333

>>9619124
I'm already connected to it, kept my eyes open 'cause i'm interested in the project.

We should keep this thread going as a sort of general to garner support from other /sci/-anons as it would help get more volunteers and raise awareness within /sci/.

I'm debating looking at /biz/-fags for the marketing side of things but those homos can't even stock market right so i'm not too sure about them...

Already doing my research and seeing where i can help.

Genetic Eng !neEr...dvM Sun Mar 25 16:52:47 2018 No.9619361

>>9618314
>>9618317
It looks like I should be able to rename it.

>>9618314
I'm still working on that list and it's by no means finished. Were you looking for a specific type of work?

>>9618505
Depending on how tight finances are, it could be as simple as hooking the electrodes up to a DAQ. Otherwise I can do it by hooking up an amplifier to a microntroller and using the onboard AD converters. The signal is on the order of microvolts though.

>>9618576
See >>9618595. EYS most likely has a fairly long half life, so injections every 6-18 months should make RP25 curable. For healing damage caused by other injuries like retinal vein occlusions, probably one or two injections total should be enough.

>>9619124
Not yet, but I linked to this thread in the previous one.

>>9619333
I see some econ majors posting on /sci/ from time to time. I think if we can make the case that the market exists and it will be profitable to produce this drug, then investors should hopefully come to us. I really appreciate the help!

Anonymous Sun Mar 25 17:03:10 2018 No.9619388

>>9619361
if investors hop on board that would be excellent, especially if we can get this past FDA (which wont be hard, honestly you can get anything past them just short of nuclear bombs) and turn a reasonable profit, we can make millions.
Speaking of, greed will come quick and the anons who helped (and those claiming to have helped) will be demanding some kind of compensation, so we will need to add to the /biz/ oriented tasks the job of figuring out who gets payed what if we decide to go down that road.

Anonymous Sun Mar 25 17:09:06 2018 No.9619402 [DELETED]

>>9619388
Is that based on experience? It seems like the FDA can be pretty stringent, albeit less so for protein replacement therapies and rare diseases. Also one option might be to place all the proceeds in a nonprofit foundation so we don't have to squabble over who gets what. Though that would be years down the road, so we shouldn't get too far ahead of ourselves.

Genetic Eng !neEr...dvM Sun Mar 25 17:10:27 2018 No.9619406

>>9619388
Is that based on experience? It seems like the FDA can be pretty stringent, albeit less so for protein replacement therapies and rare diseases. Also one option might be to place all the proceeds in a nonprofit foundation so we don't have to squabble over who gets what. Though that would be years down the road, so we shouldn't get too far ahead of ourselves

Anonymous Sun Mar 25 17:15:48 2018 No.9619424

>>9619406
>Also one option might be to place all the proceeds in a nonprofit foundation
how does this work? I've never understood non-profit.

Also, i'm still working on this for free because its a great project and i want you to succed, but i don't think i'm the first man who wouldn't mind some moola if it were available. Maybe i'm just being greedy, nonetheless i'm going to look into those tasks and see where and if i can help.

Godspeed OP.

>>	Anonymous Sun Mar 25 23:46:21 2018 No.9620108 Bump

>>	Anonymous Sun Mar 25 23:56:30 2018 No.9620122 File: 243 KB, 1000x775, postcard.gif [View same] [iqdb] [saucenao] [google] >>9618267 Fake! But this is not! https://media.giphy.com/media/47xqMrjTiFPslA0AOK/giphy.gif - more information on gif image

>>	Anonymous Mon Mar 26 00:38:45 2018 No.9620176 All the best luck OP, you are a true inspiration.

Anonymous Mon Mar 26 01:08:22 2018 No.9620214

>>9619361
How are you going to deal with this project being associated with 4chan? People who don't browse the site usually think that all boards here are like /b/ or / pol/. This could hinder the project in early stages, though if you make it past that no one's gonna care.

>>	Anonymous Mon Mar 26 01:11:43 2018 No.9620226 >>9620214 >implying OP would say the project started on 4chan its out little secrit anon :)

>>	Anonymous Mon Mar 26 01:12:50 2018 No.9620230 >>9620226 Let's hope it remains that way.

>>	Anonymous Mon Mar 26 01:13:27 2018 No.9620231 >>9620214 all boards are like /b/ and /pol/. Every other thread is /pol/-tier (((GR))) hate or flat-earth shit.

>>	Anonymous Mon Mar 26 01:17:34 2018 No.9620236 >>9620231 True, but some boards are more /pol/ like than others. Guess we should come up with a /pol/ness coefficient for each board.

>>	Anonymous Mon Mar 26 01:23:49 2018 No.9620242 >>9620236 true. I only go on /sci/ and /lit/ which both have a solid amount of /pol/ threads, but there is at least discourse. I mean I hate black people, but /pol/ is p annoying

Genetic Eng !neEr...dvM Mon Mar 26 05:09:04 2018 No.9620434

>>9619424
>>9620214
>>9620226
>>9620230
>>9620231
>>9620236
>>9620242
I am beginning to have second thoughts about posting this as a 4chan-centric project.

I really want to keep money and politics totally out of this. If it would make everyone happy, I will literally withdraw any money this drug makes and livestream me burning it in a huge bonfire. I only care about making this treatment work, and I can't afford to let anything get in the way of that. I watched what happened with the Tox project on /g/ and I don't intend to repeat their mistakes. That was a very promising piece of software that got destroyed by mentally ill concern trolls.

I think what I will do going forward is continue to work on this privately and post occasional updates as things progress.

>>	Anonymous Mon Mar 26 05:24:01 2018 No.9620446 >>9620434 It is possible to keep politics out of this since your project is unrelated to them, if we can make it past the early stages without a disaster everything will be fine

Anonymous Mon Mar 26 05:31:03 2018 No.9620457

>>9620434
The previous posts do bring up some valid problems that might arise when the project gets bigger. For now the biggest danger is that people might dismiss the project on the grounds that it's from 4chan so we should hide our involvement as much as possible.

The money issue is not really an issue, though. Just say that the goal is a working cure, not profit (basically what you just posted)

Anonymous Mon Mar 26 05:59:33 2018 No.9620482 [DELETED]

>>9620446
Tox actually got really far before the concern trolls killed it. It was a secure messenger app like Telegram, but before Telegram existed. They got quite a bit of tech news coverage and were even an open source project that was sponsored by Google at one point. But then this one troll developed a pathological obsession with the dev team and launched this huge organized FUD campaign against the project, as well as doxxing the devs and harassing them in real life, culminating in getting one of them fired from his job. This was all because they had tried to do something successful on 4chan and that made the troll jealous.

>>9620457
If I can find a legal way to do it, I will actually burn the money. I'm not even joking.

Genetic Eng !neEr...dvM Mon Mar 26 06:01:55 2018 No.9620485

>>9620446
Tox actually got really far before the concern trolls killed it. It was a secure messaging app like Telegram, but before Telegram existed. They got quite a bit of tech news coverage and were even an open source project that was sponsored by Google at one point. But then this one troll developed a pathological obsession with the dev team and launched this huge organized FUD campaign against the project, as well as doxxing the devs and harassing them in real life, culminating in getting one of them fired from his job. This was all because they had tried to do something successful on 4chan and that made the troll jealous.

>>9620457
If I can find a legal way to do it, I will actually burn the money. I'm not even joking

Anonymous Mon Mar 26 06:20:01 2018 No.9620500

>>9620485
Interesting, I googled info about Tox and all I found was a scandal that someone misused the project funds since you'll be the only one in charge of the crowdfunding campaign I figured that this won't be an issue.

For now you're successfully avoiding doxxing by using a throwaway github account, the only danger to you at the moment is those anons that you emailed (I'm one of them, but don't worry, will send you an email later). Anyway, doxxing will become an issue once the crowdfunding campaign is online so we should think of a way to circumvent that.

Anonymous Mon Mar 26 06:52:41 2018 No.9620564
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>>9618267
You know what? I am just going to sit here and follow you.
This might actually be crazy enough to work.
Also want to read all the "le 200 pound Hacker 4chan cures blindess" articles the MSM would be forced to write.

>>	Anonymous Mon Mar 26 07:30:34 2018 No.9620598 File: 41 KB, 827x469, 1518348635662.png [View same] [iqdb] [saucenao] [google] >>9618267 Can you give a rough outline of your roadmap for this?

Anonymous Mon Mar 26 09:40:12 2018 No.9620740

The FDA is slow as shit, anon. Opening a case costs millions. Won't get approved for years.

Better idea: tell me how zebrafish study goes. I have human subjects ready to go. Skip approval bullshit and prove it works on people first. Open source/diy instructions in braile. Change the world and fuck the money. Don't become a startupfag.

Anonymous Mon Mar 26 09:50:50 2018 No.9620751

>>9620740
You're probably not serious, but I'l bite:

What kind of human subjects? Hobos from the streed probably would not suffice, though it's up to OP to decide.

Even if we choose to skip FDA approval we still need a proof of concept so zebrafish or other animal trials are a must. Also this project is already open source you can do some of the open tasks yourself if you want (https://github.com/EyesOpenCure/EyesOpenProject/wiki/List-of-Open-and-Ongoing-Tasks).). Doing them will make OP less reliant on crowdfunding.

>>	Anonymous Mon Mar 26 10:42:14 2018 No.9620827 >>9620751 Lol. Not hobos. Yes, animal tests first, then I have patients that fit this risk profile. I'll keep an eye on progress.

>>	Anonymous Mon Mar 26 10:46:13 2018 No.9620833 >>9618267 seems nice but I only know a bit of physics, if I eventually learn something about biology I will help with some ideas

>>	Anonymous Mon Mar 26 10:54:22 2018 No.9620843 >>9620827 Well first we need to get to those animal tests anyway so this topic will come up later if at all (maybie FDA approval will prove easier to get >>9619388 )

>>	Anonymous Mon Mar 26 11:18:02 2018 No.9620873 >>9620598 Is that a prototypeproject banner? Pretty good, but the text is a bit mess so still needs improvement.

>>	Anonymous Mon Mar 26 11:22:46 2018 No.9620881 File: 35 KB, 827x469, 1507540562279.png [View same] [iqdb] [saucenao] [google] >>9620873 Here is a fixed version. Was done in Adobe illustrator, would glady share the .ai .svg or whatever if anyone wants it.

>>	Anonymous Mon Mar 26 11:38:43 2018 No.9620902 >>9620881 You can send it to me, I'll relay this to some of my more graphic-savvy friends. Will post their versions as well. email: omon12257@gmail.com

>>	Anonymous Mon Mar 26 12:29:36 2018 No.9621012 >>9620902 Mail was sent.

>>	Anonymous Mon Mar 26 12:33:41 2018 No.9621024 >>9621012 Mail recieved, thanks.

>>	Anonymous Mon Mar 26 16:18:16 2018 No.9621468 >>9620564 If this succeeds the benefits would go far beyond confused media. Best of luck OP.

Anonymous Mon Mar 26 16:35:53 2018 No.9621505

>>9618267
>https://www.sciencedaily.com/releases/2017/06/170606095033.htm
I'll sign up to be a test subject. If you want my contact, just let me know. I, too, am a scientist in need of this therapy, and I will sign a waiver freeing you of any responsibility for my decision to test it on myself.

>>	Anonymous Mon Mar 26 16:37:50 2018 No.9621510 how common is the condiion that would be cured anyways. What portion of blindness cases are we talking here?

>>	Anonymous Mon Mar 26 17:37:09 2018 No.9621675 >>9618267 Mate, we might actually be more than a shitposting website for once.

>>	Anonymous Mon Mar 26 17:37:16 2018 No.9621677 Grant us eyes, grant us eyes. Plant eyes on our brains, to cleanse our beastly idiocy.

>>	Anonymous Mon Mar 26 20:02:33 2018 No.9621973 >>9621510 Fairly rare, RP in general seems to affect 1 in 4000 persons. Not too certain on RP25 specifically however.

>>	Anonymous Mon Mar 26 20:53:51 2018 No.9622058 I’m not surprised. Wasn’t there a guy that had a theoretical cheap cure for cancer I saw him post here a year or so ago by using DNP and burning out the mitochondrial membrane. Anyone have the link?

Anonymous Mon Mar 26 22:03:15 2018 No.9622176
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>>9620485
>some autistic retard out of jealousy fucked up a pretty good idea
https://raidforums.com/hoa/
is he loved anon? i'm pretty sure /g/ grew such a hate-boner for the faggot that they went ahead and got revenge somehow?
Either way, autism speaks, so just safety-wrap in autism-proof tinfoil OP. Definitely wise to move this off of 4Chan, and #1 do NOT give your email to any more anons, have them contact you indirectly through the github, and make sure that that is disconnected from any accounts you use, use a new password and everything else too. As for all other anons involved in the project, do likewise.

And OP, money may become an issue in the future because jealous is human nature. Burning the money would only make those interested in it even angrier because you took what they saw as their share and destroyed it. The best way to proceed in regards to human greed when it comes is to figure out some agreement based on contribution, you can keep majority of proceeds because you came up with the idea and are doing the majority of the grunt-work.

Another option is to make the thing free, but then that opens the flood-gate for people to steal your working prototype and profit off of it themselves, and believe me when i say people WILL find a way to make a profit off this.

I fear such things are ahead, let us pray that we dodge those bullets when they come...

>>9620500
>it was mismanagement of funds
oh, then this won't really be a problem so long as OP or a biz-anon figures out how to spend for the project.

Also Doxxing is easily avoidable, just make throw-away accounts, keep everything secrit and private, and never establish connections to friends, family, or your personal account in any way.
Also, when the trolls come we can just use misdirection and distractions and false leads to keep them off our ass, For instance OP, i know you attend Univeristy of Ottowa and your name is Stephane Aris-Brosou.

Anonymous Mon Mar 26 22:27:20 2018 No.9622244
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>>9620598
The motif should be an eye opening, just barely open, looking just barely at the centre of the image, slightly off to the right. Even a photograph of a normal persons eye is good enough for this project, but damn that logo of yours is ugly.

Pic related, KISS, we want something thats catchy and grabs the audiences attention, provocative but not offensive, to really make them think. Get the point forward clear and simple, i tried a slogan but its a bit weak.
Other than that fugly eye of yours your promotional content is acually really good.

>>	Anonymous Mon Mar 26 22:35:02 2018 No.9622270 >>9618267 Went ahead and forked the github in case something happens to it and to keep a copy so i can sate the autistic forces that make me ctrl+s my word documents at least 15 times in a row.

>>	Anonymous Tue Mar 27 00:07:02 2018 No.9622412 >>9622176 Or he could use throwaway email accounts as well.

>>	Anonymous Tue Mar 27 04:14:47 2018 No.9622699 >>9622244 A combination of your image and his slogan could be an initial project banner.

>>	Anonymous Tue Mar 27 04:50:12 2018 No.9622719 File: 89 KB, 1280x720, 1516479916084.jpg [View same] [iqdb] [saucenao] [google] >>9622244 >but damn that logo of yours is ugly Never said that I am actually good at that. That said, I agree, just wanted to do one.

Genetic Eng !neEr...dvM Tue Mar 27 05:12:21 2018 No.9622743

Day 26:

Made a ton of progress on the whitepaper, but unfortunately I don't think I'll be able to finish in time for tomorrow. I should have it typeset in LaTeX by the end of the week though, which will make showing this project to researchers and investors much easier.

>>	Anonymous Tue Mar 27 05:37:56 2018 No.9622772 >>9622743 Do you have any background in molecular biology?

Genetic Eng !neEr...dvM Tue Mar 27 05:39:07 2018 No.9622774

>>9620500
>>9622176
If I recall correctly, irungentoo didn't even steal the funds. He bought himself a laptop to write code on, but then the trolls latched onto that, contacted a bunch of media outlets, and turned it into this huge FUD campaign. The Tox Foundation had no other choice but to disavow him because of the firestorm. It was some real-next-level trolling, and honestly I think state actors may have been involved since this was around the time of the Snowden leaks.>>9620564

>>9620564
>>9621468
Thanks anons!

>>9620598
I'm trying to get the whitepaper done this week, and then I hope to have the detailed plan vetted by experts in the next two weeks. Next, about a month of crowdfunding, and another two weeks after that until the first supplies arrive. Pharmokinetic tests will probably start mid-May (hopefully sooner), immediately followed by a proof of concept study in modified zebrafish that concludes in early July. If all goes well then I spend another month securing venture capital funding and hopefully get to hire some of you guys full time.

With proper funding, I'm going to redo the zebrafish experiment, but with better methodology so that it will be admissable as an FDA preclinical trial. For protein replacement the FDA wants two separate three month animal trials, so I'm going to try to run both concurrently to save time. The second trial will probably be in rabbits to determine dosing. Animal trials should be done some time in December, and the review of the Investigational New Drug Application will probably take another month for them to review.

Phase 1 clinical trials can hopefully begin in February or March 2019. If we can get the FDA to fast track the approval process on the basis of this being a treatment for a rare and serious disease, it could potentially be on the open market by Q3 2020.

>>	Anonymous Tue Mar 27 05:42:49 2018 No.9622776 >>9622743 Do you have a mail address? I'd like to talk to you in private.

>>	Anonymous Tue Mar 27 05:46:01 2018 No.9622786 File: 575 KB, 5200x3467, 1494401493900.png [View same] [iqdb] [saucenao] [google] >>9622774 >Q3 2020 Godspeed to you, anon. Make us all proud

Genetic Eng !neEr...dvM Tue Mar 27 05:56:20 2018 No.9622800

>>9620740
The FDA special provisions that make it easy to create treatments for rare diseases, and protein replacement therapies are really easy to get approved on top of that. We can actually get the FDA to pay us $15mil a year to finance clinical trials, and apply for fast track approval that would get it on the market in 1.5 years.

That being said, where are you located? I'm not sure how much I can do without getting arrested, but I would like to help once I know that this treatment isn't dangerous.

>>9621505
What sort of retinal disease do you have? Again, I need to at the very least make sure this is safe first.

>>9621510
The initial therapeutic target is RP25, which will make getting it through the FDA easiest because then it will qualify as an orphan drug. Depending on how effective EYS is at regenerating retinal tissue, it may be able to repair damage from injuries like retinal vein occlusions and infections. Possibly age-related macular degeneration too, but really it's anyone's guess at this point because nobody has tried this yet.

>>9622058
I too would like to learn more about this.

>>9622776
eyesopencure@gmail.com

>>	Anonymous Tue Mar 27 06:03:53 2018 No.9622808 >>9622800 I've been following your posts op keep up the good work, and remember that no important thing was achieved without setbacks so don't loose your motivation in case something does happen.

>>	Anonymous Tue Mar 27 11:29:38 2018 No.9623210 >>9622800 Having a dedicated project email is a good choice. Also, what do you think about the project banner ideas thrown around by a couple of anons in this thread?

>>	Anonymous Tue Mar 27 11:40:56 2018 No.9623223 >>9622719 >sadanimegirl.jpg its ok anon, you tried

>>	Anonymous Tue Mar 27 12:37:13 2018 No.9623338 >>9620434 If you do make money, don't burn it. Throw it all into other research projects, your own or others.

>>	Anonymous Tue Mar 27 16:50:38 2018 No.9623944 >>9622786 This deadline may be a little too optimistic, but still - let's go for it.

Genetic Eng !neEr...dvM Tue Mar 27 18:21:19 2018 No.9624166

So anons, someone from a certain news outlet that you've probably heard of saw this thread and contacted me about doing a story. I don't want to jeopardize the project by turning this into some kind of attentionwhore thing about me, so I haven't responded yet. However at the same time, media attention could be really useful for securing funding.

What should I do?

Genetic Eng !neEr...dvM Tue Mar 27 18:29:00 2018 No.9624182

>>9622786
>>9623944
I agree that it's ambitious, but we should aim for early delivery in order to speed things along as much as possible.

>>9622808
Thanks anon. I'm expecting issues to come up, but I'm going to keep working on this as long as I can.

>>9623210
It's progress, though I think more color might be good. If we can figure out a coordinated color scheme I can help make a website with matching colors.

>>9623338
I have a lot of other projects that I would like to put the proceeds towards, but given the choice between having this project die in controversy and throwing the money away, I would rather throw the money away. I probably can't burn the money directly, but I could buy some priceless painting and burn that instead. Or I could put it all in a dufflebag and hand it to a random homeless person. At least then it would be doing some good.

Anonymous Tue Mar 27 18:29:50 2018 No.9624183
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>>9624166
Tell him you will speak anonymously about it to him and just about the project itself, for now.
Also tell him that you don`t want to jeopardize it by turning this into some kind of attentionwhoreing.
Also (just to make sure that this is clear) tell him that you aren`t up for (pre-clinical trial) human experiments, like some people suggested ITT.

Anonymous Tue Mar 27 18:40:28 2018 No.9624205

>>9624166
make sure they DO NOT do the following:
>reveal your true identity
>mention human trials or anything that could garner too much controversy

the goal with this opportunity should be to let the public know about the goal to cure sight and nothing more. Make it explicitly clear that this is for eyes only, does not give people immortality, doesn't make designer babies, etc. Its more like a genetic band-aid than anything.

Also you should probably use this service for the funding, it shows how the funds plan on being used and so on:
https://experiment.com/

with all this in mind go for it, publicity is great for getting financial or other support. Make sure to do what >>9624183 says, just let them know you don't want this to spoil the project.

Godspeed anon.

>>	Anonymous Wed Mar 28 00:29:37 2018 No.9624961 >>9624166 What the anons above me said if the news site is reputable and doesn't publish clickbait

>>	Anonymous Wed Mar 28 01:57:53 2018 No.9625117 >>9618267 I'm seeing the thread for the first time, but I gotta say this is great work you're doing OP.

>>	Anonymous Wed Mar 28 05:53:41 2018 No.9625421 >>9624182 Maybe we should go for the visible light spectrum motif for the banner? Just make sure that the colors aren't too bright and flamboyant.

Anonymous Wed Mar 28 06:14:36 2018 No.9625444
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>>9624182
I think using the money for other projects (if there is any real amount of money left anyway) is fine as long as you say so clearly from the start.
Anything else is a lose for all of us.

Also, before anyone flames me again, I know that these are shit, but I like making them.

>>	Anonymous Wed Mar 28 06:17:49 2018 No.9625451 >>9625444 Well, the idea for using the visible light spectrum as a coloring theme is quite nice. Maybe someone with better artistic skills will improve these.

Genetic Eng !neEr...dvM Wed Mar 28 06:41:01 2018 No.9625486
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Day 27:

A lot happened today. Someone donated pic related, which is a really nice magnification lamp and should be very useful for injecting and wiring fish. I'm going to start putting together a work area this weekend.

Also, I met with that ophthalmology researcher and he agreed to review the whitepaper once it's finished. Also he sounded open to the idea of letting me use some of his facilities.

I responded to the journalist who contacted me yesterday too.

Genetic Eng !neEr...dvM Wed Mar 28 06:51:03 2018 No.9625501

>>9624183
>>9624205
>>9624961
Email sent. I'm going to try to stay anonymous for at least this first article.

>>9625117
Thanks anon!

>>9625421
>>9625444
>>9625451
I like the idea of the the color spectrum motif! What do you guys think about a circular black pupil looking at the viewer, surrounded by a rainbow colored iris, and an off-white sclera? It might look pretty good as a minimalist logo without any black border lines.

>>9625444
We'll see what everyone's consensus is and then I'll end up doing that. Giving it to charity would probably be best, but ot wouldn't send as much of a message as setting it on fire.

>>	Anonymous Wed Mar 28 06:51:40 2018 No.9625503 File: 42 KB, 478x612, 114337656.jpg [View same] [iqdb] [saucenao] [google] >>9625486 Godspeed anon, may good fortune bless this project.

>>	Anonymous Wed Mar 28 07:05:17 2018 No.9625521 >>9625501 >What do you think about a circular black pupil? This could work, gonna try to make a prototype.

>>	Anonymous Wed Mar 28 07:18:08 2018 No.9625540 I worked on creating transgenic zebrafish showing gut florescence. Need any assistance?

>>	Anonymous Wed Mar 28 07:19:31 2018 No.9625543 >>9618267 What do you have in mind for delivery? Check out this article: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4250513/

>>	Anonymous Wed Mar 28 08:17:59 2018 No.9625608 OpenEYS IS the perfect name for this

Anonymous Wed Mar 28 09:12:44 2018 No.9625673
File: 55 KB, 750x750, EYS.png [View same] [iqdb] [saucenao] [google]

>>9625501
Here's my quick attempt at a minimalistic prototype project logo.
It's not that good since I couldn't get a proper non-elliptical eye shape and the rainbow patttern should be radial, imo.
If someone with more design experience could improve it, that would be great as this is literally my 4th time doing graphic design.

>>	Anonymous Wed Mar 28 09:18:03 2018 No.9625677 Programmer here, if you need any code done I'd love to help. It's great to see a motivated person work hard on something.

>>	Anonymous Wed Mar 28 09:31:30 2018 No.9625696 File: 43 KB, 906x571, 1517860224316.png [View same] [iqdb] [saucenao] [google] >>9625673 Something more akin to this?

>>	Anonymous Wed Mar 28 09:38:26 2018 No.9625704 >>9625696 Pretty much, thank you. Now let's wait and see what OP thinks.

>>	Anonymous Wed Mar 28 09:45:35 2018 No.9625717 >>9618267 Biotech engineer here, I'm in. Keep this thread alive, I'll read it when I get back home

>>	Anonymous Wed Mar 28 09:47:52 2018 No.9625723 File: 22 KB, 758x474, 1503273219795.png [View same] [iqdb] [saucenao] [google] >>9625704 Forgot about the off-white sclera, here is v.2

>>	Anonymous Wed Mar 28 09:54:58 2018 No.9625732 >>9625723 imo the one with the border is better, but the final decision is up to the op, both are good. Also >>9620881 has a pretty good slogan.

>>	Anonymous Wed Mar 28 10:09:57 2018 No.9625741 >>9625677 There is a Math/Physics/CS section in the list of open tasks: https://github.com/EyesOpenCure/EyesOpenProject/wiki/List-of-Open-and-Ongoing-Tasks

>>	Anonymous Wed Mar 28 13:19:30 2018 No.9626078 >>9625486 When can we expect an article to come up?

>>	Anonymous Wed Mar 28 16:41:39 2018 No.9626484 Bump.

>>	Anonymous Wed Mar 28 17:46:10 2018 No.9626589 Keep this thread alive. Bump

Anonymous Thu Mar 29 00:17:33 2018 No.9627265
File: 2.20 MB, 4032x3024, IMG_0480.jpg [View same] [iqdb] [saucenao] [google]

I went ahead and left an advertisement in my university library.

Plan on getting more slips of paper and putting them in biology, business, etc books strategically so that 4th yr students working on their thesis runs into it.

Anonymous Thu Mar 29 01:39:04 2018 No.9627388

>>9627265
When the sorta-final design of the logo is done - I'll make a printable version of that, with the logo, link, QR code and a short description like :"OpenEYS (Eyes Open) - open source protein replacement therapy project aiming to cure blindness"

Anonymous Thu Mar 29 05:24:50 2018 No.9627629

>>9627388
It's not exactly curing blindness, but curing a genetic disease that happens to cause it so maybe it should say something like
>OpenEYS (Eyes Open) - open source protein replacement therapy project aiming to eliminate a blindness-causing genetic disease.

>>	Genetic Eng !neEr...dvM Thu Mar 29 05:35:04 2018 No.9627638 Day 28: Spent a good chunk of the day responding to interview questions. Transcript: https://pastebin.com/9nNdA0Le Top priority right now is finishing the whitepaper and wiki.

>>	Anonymous Thu Mar 29 05:53:55 2018 No.9627646 >>9627638 Good to see this project getting some publicity. Will you link the article once it's written? Also, as someone on github already mentioned, the whitepaper link in the wiki's homepage just redirects to the homepage instead of the whitepaper.

Genetic Eng !neEr...dvM Thu Mar 29 06:01:39 2018 No.9627652

>>9625503
Thanks anon!

>>9625521
>>9625673
>>9625696
>>9625723
I really like that last one! If we could get the colors to vary perpendicularly to how they currently are, so that they resemble the striations in the human iris, then I think that logo would be perfect. We will also want to set the background to be the alpha channel so that it's just a floating eye.

>>9625543
Nanoparticles seem interesting, but it sort of seems like they might be intended for a different class of drugs than what we're attempting. I may be wrong, but it mainly seems like they exist to keep small molecule drugs from diffusing away too quickly. EYS is already huge, so I'm not sure how much benefit they would be. It's definitely worth looking into some more though.

>>9625608
That's another good one. We should put it to a strawpoll.

>>9625677
>>9625741
>>9625717
There's plenty of work to go around! I'll update the list of tasks with some more content.

>>9626078
Probably this weekend on Vice. It's originally for the French edition though, so I'm not sure if it's going to get republished in English.

>>9627265
>>9627388
That's a really good idea! Please consider submitting the flyers to the git repository when you end up making them.

Also I really need to finish the wiki ASAP before a bunch of people start looking at it.

>>9627629
It remains to be seen whether EYS will have all of the same regenerative properties of agrin/perlecan, but the possibility is there for it to treat other injuries.

>>9627646
Absolutely! Also that link is just a placeholder because I'm still drafting the whitepaper and it's not really in a state for people to look at yet. I'm aiming to have it done by tomorrow night.

>>	Anonymous Thu Mar 29 06:49:23 2018 No.9627768 >>9627652 Will you have the wiki done by tomorrow as well?

Anonymous Thu Mar 29 06:55:56 2018 No.9627774
File: 121 KB, 548x258, 1522112779426.png [View same] [iqdb] [saucenao] [google]

>>9627652
>I really like that last one! If we could get the colors to vary perpendicularly to how they currently are, so that they resemble the striations in the human iris, then I think that logo would be perfect. We will also want to set the background to be the alpha channel so that it's just a floating eye.
Ay boss.

>>	Anonymous Thu Mar 29 06:58:11 2018 No.9627779 >>9627774 Holy shit, that's good.

>>	Anonymous Thu Mar 29 07:03:43 2018 No.9627783 >>9627779 Can't really take credit for it though, pretty much the only thing I did was lay a high-def image of an iris under the rainbow layer

>>	Anonymous Thu Mar 29 07:05:46 2018 No.9627788 >>9627783 Still, looks really good. No one here can really take credit for anything (except the tripfags).

>>	Anonymous Thu Mar 29 08:31:37 2018 No.9627882 >>9627652 Are you french?

>>	Anonymous Thu Mar 29 08:43:28 2018 No.9627894 >>9627882 Probably not, in the earlier thread op said that he's based in the US. I too am wondering why VICE chose to publish the story in their french edition.

>>	Anonymous Thu Mar 29 08:58:32 2018 No.9627912 >>9627774 http://rapidfireart.com/2013/05/08/how-to-draw-eyes/ look at the shape. eyes aren't perfectly shaped like almonds.

>>	Anonymous Thu Mar 29 09:09:15 2018 No.9627920 >>9627912 I don't think that a very realistic eye shape is that necessary. It's the symbol that's important.

>>	Anonymous Thu Mar 29 11:13:10 2018 No.9628096 >>9627912 >>9627920 I think that a symetrical shape is actually better for a minimalistic logo. There are well known exampels (think the all-seeing eye) that make it instantly recognizable

>>	Anonymous Thu Mar 29 12:51:11 2018 No.9628293 >>9627894 Reading both edition from time to time they seem not to have too much overlap. Maybe when their editiorial map is full they ship stories to their other publications

>>	Anonymous Thu Mar 29 16:21:09 2018 No.9628748 Can someone redbull me on what happened to Tox? I remember seeing it on /g/ a while ago, looked promising.

Anonymous Thu Mar 29 16:21:26 2018 No.9628750

>>9627646
You handled that interview very well also glad that the interviewer didn't place that much emphasis on the project's place of origin. Some other news outlets would have definitely touted the whole 400 pound hacker, named 4chan thing instead of focusing on what the project's about.

>>	Anonymous Thu Mar 29 16:24:59 2018 No.9628756 >>9628750 Link?

>>	Anonymous Thu Mar 29 16:31:31 2018 No.9628768 >>9628756 Nvm i am retard

>>	Anonymous Thu Mar 29 23:42:09 2018 No.9629845 Bump

Anonymous Fri Mar 30 00:36:53 2018 No.9629948

We should get an official site up and running for this project, most normies will have no clue what's going on in the wiki and will not be able to glean what its all about.

I'm thinking it could be a great page to QA normies, get them hyped, and to redirect them to fundraiser and git page if they want to contribute.

We should add this to the CS section and see if we can get >>>/g/wdg/ involved.

I have no experience but atm i'm working on a wix page to be there temporarily until someone can make a MUCH better site. Tell me how it should be.

Anonymous Fri Mar 30 00:37:55 2018 No.9629954
File: 14 KB, 619x226, Format.png [View same] [iqdb] [saucenao] [google]

>>9627388
If you can that would be fantastic!

What are the logo ideas so far?
>an eye with light coming through, fracturing as it comes out the other end into colour
>an eye with a rainbow iris (like >>9625696)

If you have any graphic designer friends that would be great.

In the meantime we should figure out logo, slogan, and format of advertisement.

Slogans because by using a short catchy phrase we can help it stick (help us cure blindness/ an YS a day helps the blindness go away/ will you help us save sight/ Putting the EYS into Eyes wide open/ etc). Logo for visual aid, recognition and to catch attention, format to make it easy to get involved, etc.

Pic related is my format suggestion, engish readers rend to read from left to right so they will see Logo -> Project name -> description -> URL -> QR code

Btw what do we plan on using the QR code for?

Anonymous Fri Mar 30 00:41:34 2018 No.9629960

>>9627629
>>9627652
It doesn't matter so much if EYS necessarily cures all blindness or just this specific type of blindness, but tell me wheich is more marketable:
>Cure blindness
>Cure type 292 blindess is the subcutanial retenospheroid mega-lobe
the first one. That's why it makes a good slogn: breif, to the point, and exciting. Gets the listeners attention and thus the (You)'s we need for the project to gain notoriety.

We should add a marketing campaign to the list of tasks in the github page probably i think.

Anonymous Fri Mar 30 00:48:23 2018 No.9629973

>>9627774
Don't make the logo too advanced, minimalism has advantages in marketing because its easy to remember, distinct, and also aesthetic.

I won't deny that the radial-rainbow-iris has its advantages, (the colour is much better than the previous renditions too) but i feel like we could do better.

>>	Anonymous Fri Mar 30 01:03:35 2018 No.9629993 >>9629954 The QR could be used for either linking to the github page or the project website.

>>	Anonymous Fri Mar 30 01:08:16 2018 No.9630001 >>9629993 Website would probably be better since it can redirect to git, wiki, and fundraiser all at once, as well as give some background/info.

>>	Anonymous Fri Mar 30 01:18:48 2018 No.9630018 >>9630001 I agree, but then we'll need a website.

Anonymous Fri Mar 30 01:26:08 2018 No.9630025

>>9630018
Already working on the unofficial Wix page.
We MUST add "build official site" to the list of tasks to complete. We won't need to worry about domains too much, we can just use Git:Pages (https://pages.github.com/).).
The URL would become something simple like "https://OpenEYS.github.io/" or someething to that affect. Its free too so that removes any overhead in regards to a website. All we need is someone to design the site and host it.

>>	Anonymous Fri Mar 30 01:52:29 2018 No.9630058 Working on Description, OP mind giving me a brief paragraph on what OpenEYS is about?

Genetic Eng !neEr...dvM Fri Mar 30 05:47:30 2018 No.9630300

Day 29:

Didn't get to the whitepaper today because I came across a couple additional functions of EYS, one of which may relate to its possible regenerative properties. Still reading about it because I want to be sure, but this could be pretty interesting.

>>	Anonymous Fri Mar 30 05:57:58 2018 No.9630312 >>9630300 Link?

Genetic Eng !neEr...dvM Fri Mar 30 06:17:50 2018 No.9630335

Sorry for not responding right away: I'm juggling a lot of different aspects of the project right now.

>>9627768
>>9627779
>>9627783
>>9627788
>>9627912
>>9628096
>>9627920
>>9629973
It's not quite what I was originally envisioning, but I think it looks pretty good. I'm okay with using it if everyone else is. Also do you want to submit it to the git repo so you can get credit for making it? Or I could upload it.

>>9627882
>>9627894
>>9628293
I'm American. The guy I did the interview with is French and it looks like he's done a few other 4chan-related stories before. He said that it's initially going to be published in French only, but he thinks the English edition will most likely pick it up and translate it.

>>9628748
It got rekt by a concern troll who was hellbent on destroying the project.
>Tox leaks your IP because it connects to the internet!!!!

>>9628750
Thanks anon! I've never done any press stuff before, so I'm really curious how this turns out.

>>9628756
>>9628768
It should be out soon.

>>9629948
>>9630001
>>9630018
>>9630025
I'm not sure about Wix, but I can make a really nice website. The trouble is I'm pretty busy with a lot of other tasks too, so /wdg/ might be a good idea. Hosting it on github is also originally what I was planning to do.

>>9629954
This is a really good idea. If you have time, a professional looking business card format that we could hand to industry and academics would be really helpful too.

>>9629960
Yeah I agree. I think it's fine if clarify elsewhere, and again, there is the possibility it's a more general treatment for other retinal diseases too, but we just haven't tried it yet.

>>9630058
I think the OP sums it up best, but maybe just reworded a little bit to be less edgy.

>>9630312
It's scattered across like ten different articles, but it has to do with this:
https://en.wikipedia.org/wiki/Disc_shedding

>>	Genetic Eng !neEr...dvM Fri Mar 30 06:35:25 2018 No.9630365 >>9630300 I forgot to add: we need to make sure that zebrafish prominin and human prominin are compatible with each other. There can be some inter-species differences that keep them from sharing EYS versions with each other.

>>	Anonymous Fri Mar 30 07:37:00 2018 No.9630467 >>9630335 >Or I could upload it. Please do, if you and the rest want to use it. Knowing myself this could end up in a huge ego driven tripfag drama otherwise.

>>	Anonymous Fri Mar 30 12:07:19 2018 No.9630896 >>9630365 And how do we do that?

>>	Anonymous Fri Mar 30 12:46:09 2018 No.9630983 >>9630335 >The guy I did the interview with is French and it looks like he's done a few other 4chan-related stories Thats probably the guy that made that article about the rare-pepe auction millionaire the other week

Genetic Eng !neEr...dvM Fri Mar 30 15:25:15 2018 No.9631251

>>9630467
Sounds good, just thought I would check. Also I would rather not use a trip, but there are too many impersonators.

>>9630896
By making sure the human-fruit fly distance >> human-zebrafish distance. The fruit fly copy almost works but not completely, so also taking into consideration some other stuff I found on glycosaminoglycan evolution, I think we should be fine. But its good to double check.

>>9630983
Yeah that's him.

>>	Anonymous Fri Mar 30 15:45:35 2018 No.9631305 >>9631251 I think he was referring to himself when talking about the drama.

>>	Anonymous Fri Mar 30 16:14:56 2018 No.9631354 >>9629960 I agree, actually, just any factual inaccuracy tends to trigger my autism.

>>	Anonymous Fri Mar 30 16:27:44 2018 No.9631386 OK the temporary site is up, check it out and tell me how i can improve. https://tttt63733.wixsite.com/openeys

Anonymous Fri Mar 30 20:55:08 2018 No.9631900

>>9630335
IF you do get in touch with /wdg/ then these are the things we need on the site:

>about us
>contact us
>home
>contribute (volunteer or donate)
>white paper and other academic resources
>etc.

>>9631386 is a start but could definitely be better.

>>	Anonymous Fri Mar 30 21:44:58 2018 No.9632013 >>9631900 Might give it a try tomorrow. Do you have some content ready to be used for it? Just a fairly simple site with clean layout and the categories you mentioned for a start, right?

Anonymous Sat Mar 31 00:32:03 2018 No.9632311

>>9632013
>Do you have some content ready to be used for it?
nope i just grabbed stock photos i thought were relevant and threw them together.

>Just a fairly simple site with clean layout and the categories you mentioned for a start, right?
Yup. The goal is to use this site more as a convenient and clean intro for normies and newbies alike.

Genetic Eng !neEr...dvM Sat Mar 31 05:52:40 2018 No.9632678

Day 30:

Even though there's 50+ different genetic causes of retinitis pigmentosa, I'm beginning to think the one thing they all share in common is that they all disrupt outer segment disc renewal/ shedding. The photoreceptor outer segment that's actually responsible for capturing light wears out over time, so it naturally gets regenerated and replaced. Some napkin math shows that just a 5-10% reduction in the efficiency of this renewal process would correspond very closely with the observed rate of degeneration in most types of RP.

I went through all the different genes that cause RP and they fall into three categories:
-structural proteins involved in disc formation
-phototransduction proteins
-transcription factors and chaperones for the previous two categories

Now first of all, how in the actual fuck is a structural protein supposed to cause photoreceptor toxicity directly? That's the conventional wisdom, but I can't find any experimental evidence to back it up.

Secondly, I found this paper [1] that talks about how RP-causing mutations in some phototransduction proteins lead to constant hyperpolarization of photoreceptors, and how constant polarization mimics constant light exposure. Outer segment disc renewal depends on the body's circadian rhythm, and constant hyperpolarization/light exposure will prevent the photoreceptors from maintaining themselves. I looked up some of the other RP-causing phototransduction proteins and found the same exact thing: their mutant versions *all* lead to hyperpolarization.

If this is correct, then it has a lot of implications. EYS seems like it might have a role in stimulating outer segment disc renewal, so if that's correct, then the protein will be able to treat most types of RP. More broadly, and again only if this hypothesis is correct, then retinitis pigmentosa in general can be treated by modulating disc renewal/ the circadian rythym.

[1] https://www.sciencedirect.com/science/article/pii/S0014483583711322

>>	Anonymous Sat Mar 31 05:59:49 2018 No.9632687 >>9632678 How's the whitepaper going?

Genetic Eng !neEr...dvM Sat Mar 31 06:07:07 2018 No.9632695

>>9631386
>>9631900
>>9632013
>>9632311
>Do you have some content ready to be used for it?
Unfortunately the best I've got are some interview questions and 30% of a whitepaper.
https://pastebin.com/9nNdA0Le
https://pastebin.com/U7bHPSGn
https://github.com/EyesOpenCure/EyesOpenProject/blob/master/Whitepaper.md

>>9632687
I still have to work some more on it. I've been neglecting it because if this >>9632678 is right then it would change a lot of things about the whitepaper.

>>	Anonymous Sat Mar 31 06:10:49 2018 No.9632699 >>9632695 >then it would change a lot of things about the whitepaper In a good way?

Genetic Eng !neEr...dvM Sat Mar 31 06:19:35 2018 No.9632709

>>9632699
Yeah. I'm still reading about this, and I'm not 100% sure it's right yet, but EYS seems like it can stimulate outer segment regeneration. That would mean it could treat all types of RP, photic retinopathy, vitamin A associated retinopathy, and potentially others, rather than only being able to treat RP25.

I'm just a little bit cautious because it sounds too good to be true.

>>	Anonymous Sat Mar 31 06:23:41 2018 No.9632715 >>9632709 If you're not sure if it's correct, maybe you should finish the whitepaper as you originally intended and devote a small section to this new info to make people aware of the possibility.

>>	Genetic Eng !neEr...dvM Sat Mar 31 06:30:41 2018 No.9632725 >>9632715 Yeah, I think that's probably a fair strategy at this point. Also it's looking like he probably won't post the Vice article until Monday or Tuesday, so there should be a bit of extra time to make everything look presentable.

>>	Anonymous Sat Mar 31 06:43:32 2018 No.9632757 >>9632725 So what's the current deadline for the whitepaper and the wiki?

>>	Anonymous Sat Mar 31 06:47:32 2018 No.9632763 >>9618267 I wish you the best of luck OP. I hope that your research will aid us all in some form or another.

>>	Genetic Eng !neEr...dvM Sat Mar 31 06:52:48 2018 No.9632768 >>9632757 I think we should be fine if we get it done by Sunday. Though I keep saying "I'll have it done by tomorrow" which often doesn't pan out. >>9632763 Thanks anon!

>>	Anonymous Sat Mar 31 07:00:41 2018 No.9632780 >>9632768 Also, you've mentioned that you'll be putting together a work area this weekend. How's that going along, post pics when you finish.

Anonymous Sat Mar 31 07:07:12 2018 No.9632783

>>9632709
Don't make grandiose statements in the whitepaper without any evidence to back it up, if your theory turns out to show some merit, it can always be added in later for a bit of extra publicity if the project starts to run out of steam.

Just found out about this project and I wish you the best of luck. If I can help in any way I will do so.

>>	Anonymous Sat Mar 31 07:11:13 2018 No.9632788 >>9632783 This. We need to focus on just treating the disease that you have, such findings, for now, should be added to something like "future possibilities" section until they are proven.

>>	Anonymous Sat Mar 31 07:39:39 2018 No.9632850 Web developer here from /wdg/, I can make you guys a website in a day. Just want to ask a few questions: >Final name of the project? >Is there a web team already that is working for the project?

>>	Anonymous Sat Mar 31 07:45:23 2018 No.9632860 Is there a project discord or anything?

>>	Anonymous Sat Mar 31 07:47:10 2018 No.9632866 I think someone should make a discord server for this.

>>	Anonymous Sat Mar 31 07:48:56 2018 No.9632869 >>9632866 Good idea, a live chat would allow for a faster exchange of ideas.

Anonymous Sat Mar 31 07:52:17 2018 No.9632876

>>9632850
OP mentioned something like making a straw poll for the final name of the project. Currently the candidates are:
>EyesOpenProject
>EYS WideOpen
>Open EYS

And no, there's no web team forking on this as far as I know. Save from a demo site made by this anon:
>>9631386

>>	Anonymous Sat Mar 31 07:53:51 2018 No.9632883 >>9632876 Alright, in the mean time, I'll try to make something. I'll use Open EYS since I like it the most.

>>	Anonymous Sat Mar 31 07:55:57 2018 No.9632889 >>9632860 >>9632866 Fuck voice chat. Use IRC.

>>	Anonymous Sat Mar 31 08:21:15 2018 No.9632959 >>9632883 Also, I need a write ups. What are the sections of the page? >Home >About Contribute >etc. >what do I write >icons? images?

Anonymous Sat Mar 31 08:29:18 2018 No.9632988

>>9632959
This post is a good guideline for the sections
>>9631900
The op is basicly the project's about section also take a look at these links:
>https://pastebin.com/9nNdA0Le
>https://pastebin.com/U7bHPSGn
where op describes the project to an interviewer. Also the whitepaper is a good technical source.
>https://github.com/EyesOpenCure/EyesOpenProject/blob/master/Whitepaper.md
Even thogh it's unfinished.

The project's logo is shaping up to be an eye with a rainbow colored iris and an off-white sclera. Like in these posts:
>>9625723
>>9627774

>>	Anonymous Sat Mar 31 10:22:26 2018 No.9633236 >openeysweb.github.io Finished the skeleton. I need help on write ups. This is just a temporary website for demo purpose. OP's Github will be used when everything is finalized.

>>	Anonymous Sat Mar 31 10:29:03 2018 No.9633257 >>9633236 Looks like a pretty good start. The Contribute section should link to the list of open tasks >https://github.com/EyesOpenCure/EyesOpenProject/wiki/List-of-Open-and-Ongoing-Tasks

>>	Anonymous Sat Mar 31 11:24:37 2018 No.9633351 >>9633236 >I need help on write ups I think that only OP will be able to really help you on that. His email for this project is: >eyesopencure@gmail.com

>>	Anonymous Sat Mar 31 11:54:30 2018 No.9633407 >>9633236 also, it would be nice if we could have a graphic artist. It would give more life to the website if there's pictures. I'm also open to suggestions in layout, etc. I can pretty much make anything, as long as it's not super complicated.

Anonymous Sat Mar 31 12:16:23 2018 No.9633458

>>9633407
We don't need anything too complicated. The website should be something akin to an introductory resource for general public and would-be contributors and serve as a link hub for people wanting to find out more (links to github and crowdfunding campaign).

>>	Anonymous Sat Mar 31 13:35:19 2018 No.9633613 I learned about this project from /wdg/, I think what you all are working on is very cool. I don't want to step on anyone's toes, but would you all mind if I took a shot at a static landing page draft?

>>	Anonymous Sat Mar 31 13:45:56 2018 No.9633647 >>9633613 You're welcome. I'm sure other anons here will share my sentiments.

>>	Anonymous Sat Mar 31 14:01:00 2018 No.9633686 >>9632678 >how in the actual fuck is a structural protein supposed to cause photoreceptor toxicity directly? i suppose its like how a minor change in the structure of hemoglobin can lead to sickle-cell disease?

>>	Anonymous Sat Mar 31 14:07:15 2018 No.9633707 >>9633257 and also a donate page when we have one.

>>	Anonymous Sat Mar 31 14:09:24 2018 No.9633716 >>9633458 Would be nice if it looked nice too though, would make it look a bit more professional.

>>	Anonymous Sat Mar 31 14:10:54 2018 No.9633721 >>9633613 go for it anon.

Anonymous Sat Mar 31 14:17:09 2018 No.9633732

Even if you succeed in humans I would think the eyes would be weak as fuck. Like someone whose legs don't work from birth that suddenly gets the ability to stand, will not be able to do so. There's sometimes years of physical therapy and that's just for some monkey limbs, no telling with the complexity of the human eye how long it might take.

Not trying to turn you off from this at all, I like what I've read so far. Just trying to help provide perspective on issues so that they can be resolved in time.

>>	Anonymous Sat Mar 31 14:35:42 2018 No.9633778 >>9633732 The difference is that (AFAIK ) this is first and foremost about an illness/defect that causes blindness later on

Anonymous Sat Mar 31 18:46:32 2018 No.9634530
File: 168 KB, 1903x1476, OpenEYS.png [View same] [iqdb] [saucenao] [google]

>>9631900

>>9632013 again

No idea how those scientific research sites usually look.
Made a really basic version, but maybe just say if this goes in the direction of what you were thinking about.
(more on the informative or stylized side?)

I also don't know how many anons now said that they were working on a site. Like 3 or 4?
Some way to coordinate this would be good, so the effort of different people doesn't go to waste.

Anonymous Sat Mar 31 19:00:08 2018 No.9634577

>>9634530
>>9634530
I like this, demo when?

>coordinate
We will see if OP adds a location into his GitHub for web commits, if not then I'll open up a project on my github to store all the different websites from all the different anons.

We could probably start a small straw-poll to determine a winning page. ATM though, just try making your own, posting the url here, and host it on a git:pages or something.

>>	Genetic Eng !neEr...dvM Sat Mar 31 19:22:44 2018 No.9634615 Sorry everyone: busy with something else right now. In the meantime I made slack group: https://openeys.slack.com/

Anonymous Sat Mar 31 19:31:17 2018 No.9634634

I'm thinking about giving this task a go for the sake of it, but am clueless what its about.

https://github.com/EyesOpenCure/EyesOpenProject/wiki/Comparative-Genomics

I have some math, CS background. How long do you think it will take me?

Also, is it ok if I do it in C?

Genetic Eng !neEr...dvM Sat Mar 31 20:29:09 2018 No.9634766

>>9634615
>>9632860
>>9632866
>>9632869
>>9632889
Here's the invite link
https://join.slack.com/t/openeys/shared_invite/enQtMzQwMzc0NjQzMTI3LWY0OTg4MzgzNzJjMmI2NzY2Njg3OTQ3MjhkMzZhYTY2MTExYzMwY2IyN2M4Y2FkZTllYjU1ODhjOTUxY2YyMDM

>>9632780
I have an old 100 gallon fish tank that I'm cleaning out and then installing some mesh dividers in it so we can separate the different control groups. Then there's the magnification lamp that I posted earlier. There will also going to be some tables and plastic sheet from the hardware store.

>>9632783
Yeah I need to research it some more. Also I appreciate the offer!

>>9632788
Yeah, I can just edit the "other applications" section slightly.

>>9632850
>>9632876
>>9632883
>>9632959
>>9632988
>>9633236
>>9633257
>>9633351
>>9633407
>>9633458
>>9633613
>>9634530
>>9634577
Looks good! I guess let's go with OpenEYS since that seems to be most popular. If we could adapt the content from these links >>9632695, that would be easiest. Otherwise I can maybe to writeups later.

>>9633686
Maybe, but how would that cause the production of toxic reactive oxygen species? It just seems kind of suspicious. Also sickle cell disease just make you clot easily, but isn't directly toxic.

>>9633732
>>9633778
It's a progressive retinal degeneration. This protein replacement therapy would stop or reverse the damage, but it probably wouldn't work in someone who is totally blind.

>>9634634
Go for it! There are few other people working on this already, so it might be good to see how far they are and collaborate with them. I'm not quite sure how long all of it would take, but there are several different smaller sub-tasks depending on how far you want to take it.

>Also, is it ok if I do it in C?
It would be extremely painful. You would have to reimplement a lot of things, like isoform prediction, that I still don't fully understand. There are quite a few higher level bioinformatics libraries out there that can do most of the heavy lifting.

>>	Anonymous Sat Mar 31 20:33:55 2018 No.9634782 >>9634615 >need invitation from administrator to access it

>>	Genetic Eng !neEr...dvM Sat Mar 31 20:36:28 2018 No.9634789 >>9634782 Try https://join.slack.com/t/openeys/shared_invite/enQtMzQwMzc0NjQzMTI3LWY0OTg4MzgzNzJjMmI2NzY2Njg3OTQ3MjhkMzZhYTY2MTExYzMwY2IyN2M4Y2FkZTllYjU1ODhjOTUxY2YyMDM

>>	Anonymous Sat Mar 31 20:45:18 2018 No.9634810 >>9634789 thanks

>>	Anonymous Sat Mar 31 22:52:58 2018 No.9635076 >>9634577 >demo when? Can post a URL tomorrow, after making it minimally responsive and filling in some content from >>9632695 and >>9634766

Anonymous Sat Mar 31 23:59:21 2018 No.9635180
File: 447 KB, 1920x1076, 1520531694811.jpg [View same] [iqdb] [saucenao] [google]

Different webdev anon here, here's my progress. I wasn't sure about which logo to use so I'm not putting one in just yet. I believe it needs a simpler one, I can take a crack at designing one myself since my background is UI/UX and design. What do you think? The photo is a free stock photo I've found so if you go with this, I'd request that credit be given in the footer to the original artist.

>>	Anonymous Sun Apr 1 00:07:18 2018 No.9635193 >>9635180 >injuries i don't thing a genetically inherited disease is an injury anon...

>>	Anonymous Sun Apr 1 00:10:23 2018 No.9635200 >>9635193 I went by the title of the whitepaper.

Anonymous Sun Apr 1 00:11:51 2018 No.9635203
File: 54 KB, 1480x832, Logo Motif.jpg [View same] [iqdb] [saucenao] [google]

>>9635180
I like the UI and black-and-white minimalism provided here, but i feel like we could use a nice logo on the left with some colour in it.
My guess would be something like that prism on pink floyd's album "Dark side of the mood" but with an eye or a strand of DNA instead of a prism. Feels like a good motif to me...

>>	Anonymous Sun Apr 1 00:12:53 2018 No.9635205 >>9635200 oh...

Anonymous Sun Apr 1 00:14:16 2018 No.9635208

>>9635203
Devanon again, I think if the logo is an eye, it should be a bit simpler and could be placed over the photo's eye. Ideally the logo would be simple and two-tone at most, so it can easily be translated to black and white for printed purposes. In that case, it would be very easy to animate slow color gradation through it on the site which would be a pretty elegant solution I think.

Anonymous Sun Apr 1 00:21:10 2018 No.9635225

>>9635208
if thats how you want to go for it, then go for it anon.

parsonally i'm not a fan of having a sad grey human face at all (or a human face in general), but i do listen to tame impala alot so take my advice with a grain of salt.

i think the eye in >>9634530 is pretty aesthetic, we have some amateur gd's who opted for a design like >>9627774 but i don;t like it cause it ain't minimal and i feel minimalism is the road to go down with some wedsites. I might be wrong though.

Anonymous Sun Apr 1 00:26:59 2018 No.9635233

>>9635225
>>9635203
https://join.slack.com/t/openeys/shared_invite/enQtMzQwMzc0NjQzMTI3LWY0OTg4MzgzNzJjMmI2NzY2Njg3OTQ3MjhkMzZhYTY2MTExYzMwY2IyN2M4Y2FkZTllYjU1ODhjOTUxY2YyMDM

>>9635193
>>9635200
>>9635205
The whitepaper is still very WIP. Also EYS has a bunch of EGF-like domains that it probably uses to stimulate outer segment renewal, which would have implications for other diseases too.

Anonymous Sun Apr 1 02:36:59 2018 No.9635597

>>9634634
By all means, go for it.
I'm one of the other anons that decided to take a shot at this task. Been in contact with op for a while now and her's the list of subtasks to this problem that he sent me.
>1. identify all the isoform sequences for EYS, perlecan, and agrin
>2. see if any EYS isoforms have a close edit distance to any known perlecan or agrin isoforms
>3. look up what those matching perlecan or agrin isoforms do in a reference manual

Anonymous Sun Apr 1 04:53:35 2018 No.9635853

>>9634766
>It would be extremely painful. You would have to reimplement a lot of things
OK then. Python it is.

>>9635597
OK, let's start at the beginning, with point 1..... Can I just use some library for the sequence ID or do I have to go through stuff like linkrelated?
https://link.springer.com/chapter/10.1007%2F978-94-017-7450-5_6

Also, is this what will essentially be done for the first of yout points?
https://stackoverflow.com/questions/46621982/using-biopython-to-retrieve-isoform-sequences-of-a-swissprot-entry

Anonymous Sun Apr 1 05:20:50 2018 No.9635901

>>9635853
I'm pretty sure biopython will do the job in point 1. I didn't get around to reading the documentation in depth yet, but it seems that biophyton doesn't have a built in Levenshtein edit distance calculator. However, there are other python modules that should do the trick, for example:
> https://pypi.python.org/pypi/editdistance
>https://pypi.python.org/pypi/StringDist
After the first step is completed we can view isoforms as strings of letters.

Anonymous Sun Apr 1 06:00:36 2018 No.9635976

>>9635901
Yeah, Biopython may not have everything, but we can use stuff like this:
http://stackabuse.com/levenshtein-distance-and-text-similarity-in-python/

I'm just trying to decide on which language to use at this point. Since Java is for transsexual faggots, I think we should go with Python.

p.s.: it's annoying this whole team BS since IDs were useful for identification to see if its the same person you're talking to.

>>	Anonymous Sun Apr 1 06:02:58 2018 No.9635982 test

>>	Anonymous Sun Apr 1 06:26:09 2018 No.9636029 >>9635976 I agree that python may be the best choice, there we have modules to do the heavy lifting.

Anonymous Sun Apr 1 07:06:58 2018 No.9636120 [DELETED]

Day 31:

I was looking at it a little bit more as I was referencing the whitepaper, and I'm pretty sure now that I'm onto something with >>9632678. I'm emailing that ophthalmology researcher to see what he thinks about it.

Also I made a little bit more progress on the whitepaper.

Anonymous Sun Apr 1 07:09:06 2018 No.9636125 [DELETED]

Day 31:

I was looking at it a little bit more as I was referencing the whitepaper, and I'm pretty sure now that I'm onto something with >>9632678. I'm emailing that ophthalmology researcher to see what he thinks about it.

Also I made a little bit more progress on the whitepaper.

Anonymous Sun Apr 1 07:10:56 2018 No.9636128

Day 31:

I was looking at it a little bit more as I was referencing the whitepaper, and I'm pretty sure now that I'm onto something with >>9632678. I'm emailing that ophthalmology researcher to see what he thinks about it.

Also I made a little bit more progress on the whitepaper.

Anonymous Sun Apr 1 07:20:21 2018 No.9636146

>>9635901
It doesn't necessarily need to be Levenshtein, just whatever distance metric is a best practice in modern bioinformatics. That's strange though. You would think that biopython would include something like that.

>>9635976
Someone else was looking at Perl's biolib, Also yeah this team thing is annoying.

>>	Anonymous Sun Apr 1 07:23:04 2018 No.9636155 >>9636146 I also thought that biopython had edit distance calculation included. Maybe I missed something.

>>	Anonymous Sun Apr 1 09:19:49 2018 No.9636418 posting in this thread so that I can follow it

>>	Anonymous Sun Apr 1 09:59:22 2018 No.9636518 >>9636146 Maybe different anons could try different edit distance calculation methods? Then we could compare theirr results.

>>	Anonymous Sun Apr 1 14:17:15 2018 No.9637211 I have been lurking since day 1 will start contributing when I get a chance. GL OP.

>>	Anonymous Sun Apr 1 14:41:48 2018 No.9637282 >>9634577 >demo when https://openeys.netlify.com/

>>	Anonymous Sun Apr 1 14:48:48 2018 No.9637308 >>9637282 Looks good, only if that logo in the upper right corner had a pastel rainbow iris as this >>9625723 is currently the op's logo of choice, still needs some improvement though like a radial iris.

Anonymous Sun Apr 1 15:22:11 2018 No.9637399

>>9637282
>Lorem ipsum dolor sit amet, consectetur adipiscing elit. Maecenas et metus ante. Sed vitae leo quam. Maecenas euismod massa a dictum consectetur. Morbi faucibus elit at fringilla eleifend. Sed varius, nisl eu lobortis ullamcorper, quam est finibus tellus, non hendrerit odio nulla eu magna. Orci varius natoque penatibus et magnis dis parturient montes, nascetur ridiculus mus. Nunc ipsum velit, consectetur vitae ex ac, blandit pulvinar elit. Sed non felis elit.

>Curabitur id dapibus nisi, non lobortis arcu. Aliquam erat volutpat. Vestibulum mollis iaculis velit, et rhoncus sapien euismod sit amet. Praesent accumsan convallis velit vitae ornare. Duis maximus luctus lobortis. Interdum et malesuada fames ac ante ipsum primis in faucibus. Pellentesque molestie ligula vitae mi cursus fringilla. Sed sit amet mauris vitae sem elementum gravida in ut metus. Proin tempus ullamcorper porta. Suspendisse consectetur eu orci in ultricies. In laoreet dui at aliquam malesuada. Morbi blandit enim vel ultrices pulvinar. Donec fringilla tortor ipsum, non porta massa congue vitae. Nulla facilisi.

>Phasellus ultricies, elit eu bibendum porta, mauris mauris euismod dolor, quis blandit mi orci eget lorem. Nam libero magna, molestie ut nisl at, elementum lacinia tellus. Sed convallis nulla dui, vitae tincidunt sem consequat sed. Praesent lobortis imperdiet venenatis. Etiam quis tortor turpis. Nullam vel libero tempus, efficitur augue sed, eleifend dolor. Nam a risus convallis mi semper vulputate. Praesent aliquam auctor ante, congue fermentum nibh scelerisque lobortis. Donec justo erat, tempor a dapibus et, efficitur nec odio. Etiam vitae dui finibus, blandit neque quis, sollicitudin magna. Sed efficitur urna ipsum, eget elementum leo feugiat et. Pellentesque habitant morbi tristique senectus et netus et malesuada fames ac turpis egestas. Duis accumsan et ex vel eleifend. Fusce lobortis arcu id turpis semper, vel tincidunt justo vulputate.

w-why all the old latin?

>>	Anonymous Sun Apr 1 15:24:55 2018 No.9637407 >>9618267 Are you the guy who had some terrible eye disease and decided to solve it? If so, congrats for keeping it up.

>>	Anonymous Sun Apr 1 15:29:03 2018 No.9637422 >>9637399 Placeholder text, I guess. Now I'm interested in a translation.

>>	Anonymous Sun Apr 1 16:21:15 2018 No.9637571 >>9637399 That's how you do placeholders in text. Look up Lorem Ipsum on wikipedia.

Anonymous Sun Apr 1 17:03:51 2018 No.9637695

>>9636155
OK, I've been going through biopythons cookbook found here:
http://biopython.org/DIST/docs/tutorial/Tutorial.html

and there are only two concepts of distance that the thing knows. One is the distance in the vector sense (e.g. Euclidian) and the other is the distance between atoms.

http://biopython.org/DIST/docs/tutorial/Tutorial.html#htoc173
http://biopython.org/DIST/docs/tutorial/Tutorial.html#htoc231

But that doesn't mean that Python can't measure the distance. See here.
https://www.python-course.eu/levenshtein_distance.php

It just means that one specific library of Python can't do it.

I think that as we progress it is important to have a lot of tools at our disposal. I think Python may be the best in this sense.

Anonymous Sun Apr 1 17:20:00 2018 No.9637741 [DELETED]

File: 49 KB, 500x277, 1-s2.0-S001216061200468X-fx1.jpg [View same] [iqdb] [saucenao] [google]

>>9636155
>>9636518
I think as long as we use whatever the current best practice distance metric is, then we should be fine. More advanced metrics would probably just let you find more similarity that a simpler metric like Hamming distance would miss.

Also we will want to try this on prominin too >>9630365 >>9630896 >>9631251.

>>9637211
Thanks anon!

>>9637282
That looks really good! Also like I mentioned in the slack, maybe we could consider tracing over some of the graphics from scientific publications in an SVG editor, and then using then using those so that we can have informative graphics on the website. If we trace it then we should be able to claim fair use. Pic related is one example, but we could also trace images of photoreceptor immunostaining and micrographs.

>>9637308
I think we should probably use whatever is most popular, but my personal opinion is radial rainbow iris > photorealistic iris > concentric iris.

>>9637407
Yep! And thanks!

>>9637695
Maybe we should try plugging some search terms into google scholar and see what other people are using.

Anonymous Sun Apr 1 17:25:48 2018 No.9637758
File: 49 KB, 500x277, 1-s2.0-S001216061200468X-fx1.jpg [View same] [iqdb] [saucenao] [google]

>>9636155
>>9636518
I think as long as we use whatever the current best practice distance metric is, then we should be fine. More advanced metrics would probably just let you find more similarity that a simpler metric like Hamming distance would miss.

Also we will want to try this on prominin too >>9630365 >>9630896 >>9631251.

>>9637211
Thanks anon!

>>9637282
That looks really good! Also like I mentioned in the slack, maybe we could consider tracing over some of the graphics from scientific publications in an SVG editor, and then using those so that we can have informative graphics on the website. If we trace it then we should be able to claim fair use. Pic related is one example, but we could also trace images of photoreceptor immunostaining and micrographs.

>>9637308
I think we should probably use whatever is most popular, but my personal opinion is radial rainbow iris > photorealistic iris > concentric iris.

>>9637407
Yep! And thanks!

>>9637695
Maybe we should try plugging some search terms into google scholar and see what other people are using.

>>	Anonymous Sun Apr 1 17:26:35 2018 No.9637762 You forgot your trip again m8.

>>	Anonymous Sun Apr 1 17:27:10 2018 No.9637763 >>9637762 The team thing is blocking it.

>>	Anonymous Sun Apr 1 17:28:36 2018 No.9637769 >>9637763 Thanks, gookmoot.

>>	Anonymous Sun Apr 1 18:17:50 2018 No.9637904 >>9637769 ITT: gookmoot causes blindness

>>	Anonymous Mon Apr 2 00:58:51 2018 No.9639074 >>9637904 dammit hiroshima nagasaki

Anonymous Mon Apr 2 05:13:32 2018 No.9639396

>>9637758
>Maybe we should try plugging some search terms into google scholar and see what other people are using.
Well, I suggest we use a proper language like either Python, C, C++ or Fortran. The reason for this is that a gazillion libraries exist to achieve a gazillion different tasks. One day we may need stuff like parallel computing or something completely unexpected and then its important that the language is developed for other fields, not just currently used by some medics.

Anonymous Mon Apr 2 05:17:37 2018 No.9639402

>>9639396
I think Python would be the best choice. It has tons of libraries to do most of the heavy lifting and is easy to code with. Fortran or C and other languages of the sort would be better in case the project needs higher computational speeds, but I doubt that a biotech project would require complex mathematical models.

>>	Anonymous Mon Apr 2 05:35:28 2018 No.9639415 >>9618267 is this also able to fix sight for non-blind people?

Anonymous Mon Apr 2 05:42:50 2018 No.9639423

>>9639415
Not really, the main goal is curing a genetic disease that causes blindness in the long run. Though OP managed to uncover some other regenerative properties of this protein so there's a possibility that such therapies are possible for other cases.

Regardless, myopia is caused by eye deformities so protein replacement therapy is not a viable solution.

Genetic Eng !neEr...dvM Mon Apr 2 06:16:12 2018 No.9639442

Day 32:

Easter ate up a lot of time.

It seems that rhodopsin mislocalization is a secondary effect of disc morphogenesis issues (disc formation issues are documented to occur before mislocalization). My guess is that this occurs because the rate of rhodopsin production outpaces disc formation and it doesn't have anywhere else to go.

Also I want to find some better papers on retinal degeneration due to constant light exposure/ circadian rhythm disruption, but so far everything has been really old and with terrible methodology. That would let me estimate the cutoff for disc renewal efficiency before retinal degeneration will start to occur.

Genetic Eng !neEr...dvM Mon Apr 2 06:29:24 2018 No.9639445

>>9639396
I just meant we could do a search to see what the preferred software libraries are for this stuff. I can also go back over the papers I read to see which ones they mentioned.

>>9639402
EYS is orders of magnitude too large to do computational protein folding, so we're stuck with much less demanding genomics-based methods for now. As long as we're doing that, then I don't think performance should be too big of a concern for this application. Especially since we only have to run it once.

>>9639415
See >>9639423
We need to do experiments before we know the full scope of applications.

>>	Anonymous Mon Apr 2 06:35:37 2018 No.9639454 Are there any simple things to do which could help you with project?

>>	Anonymous Mon Apr 2 06:53:01 2018 No.9639474 >>9639454 Ther's the outreach campaign. Any help on spreading awareness is welcome.

>>	Anonymous Mon Apr 2 13:16:14 2018 No.9640075 Cheeky bump

Anonymous Mon Apr 2 14:23:32 2018 No.9640243

>>9639445
I think that using python the approach to comparative genomics task should be something like this:
>1. Using biopython identify different isoforms of EYS, agrin and perlecan.
>2. calculate edit distances between these isoformms using StringDist https://pypi.python.org/pypi/StringDist (can calculate two different edit distance types) Levenshtein distance still seems to be the most popular one in genetics.
>3. Draw conclusions from the table of calculated edit distances.

Parts one and two seem quite doable even by people without a background in biology (like myself), for part 3 we'll need an actual biologist or someone with more knowledge on the field (like op).

>>	Anonymous Mon Apr 2 17:16:57 2018 No.9640706 If you keep this limited to /sci/ it will go nowhere. You need to make threads on other boards as well.

>>	Anonymous Mon Apr 2 17:19:34 2018 No.9640715 >>9640706 >You need to make threads on other boards as well. NOPE. spread the word by shilling lightly, but if you do threads on other boards you will piss off the other boards with your spam shit.

>>	Anonymous Mon Apr 2 17:59:45 2018 No.9640816 >>9640706 This is 4chan you're talking about........Maaaaaybe /g/.....eventually >>9639445 > I can also go back over the papers I read to see which ones they mentioned. I think that would be useful

Anonymous Mon Apr 2 22:54:06 2018 No.9641381

>>9640816
>>9640715
You guys are talking about crowdfunding for the cure of blindness... That seems like it needs to be bigger for it to be successful (I don't know much about this, haven't read the whole thread).

Also, /sci/ is equally as, if not more cancerous than a good half of the other boards don't give me that shit. I'm not talking about spreading this to /b/ ffs

>>	Anonymous Tue Apr 3 00:05:11 2018 No.9641487 >>9641381 if you feel it necessary then spread the news to the relevant boards. Just be careful which boards you choose, and avoid making threads about it if you can.

>>	Anonymous Tue Apr 3 00:18:41 2018 No.9641505 >>9641487 I won't be. It was merely a suggestion

Genetic Eng !neEr...dvM Tue Apr 3 01:23:15 2018 No.9641624

>>9639454
If you have time, take a look at https://github.com/EyesOpenCure/EyesOpenProject/wiki/List-of-Open-and-Ongoing-Tasks
It's still sort of sparse at the moment, but I'm planning on adding more.

>>9640243
Yeah that seems like a good outline. I can help dig up info on the isoforms once we find matching ones.

>>9640706
>>9640715
>>9640816
>>9641381
>>9641487
>>9641505
We're still really early in working on this so I'm not sure if we're ready to handle a huge influx of contributors yet. We'll definitely want all the help we can get, but we still need to iron out a few more details first. Hopefully we'll be ready within the next couple weeks to expand some more.

Once we're ready we might want to try reaching out to /diy/ as well. There's some pretty smart people over there.

>>9640816
It looks like the isoforms are pre-sequenced for us, so that's one less thing to worry about:
http://www.uniprot.org/uniprot/Q5T1H1#Q5T1H1-1
http://www.uniprot.org/uniprot/Q5T1H1#Q5T1H1-2
http://www.uniprot.org/uniprot/Q5T1H1#Q5T1H1-3
http://www.uniprot.org/uniprot/Q5T1H1#Q5T1H1-4

Also I found this massive catalog of bioinformatics resources, which is neat.
https://www.expasy.org/

Genetic Eng !neEr...dvM Tue Apr 3 05:08:53 2018 No.9641848

Day 33:

It looks like the relatively sudden onset of RP might be caused by a runaway positive feedback loop disrupting outer segment disc renewal. When the phototransduction cycle is degraded, it tends to keep photoreceptors from fully depolarizing back into an "off" state. So then a slowdown in disc renewal due to a genetic cause of RP might lead to some discs to wear out before they're phagotcytized by the RPE, which causes some of the phototransduction proteins to wear out and stay partially polarized, which further slows down disc renewal.

I added details for a couple more tasks on the wiki (electroretinogram design and market analysis).

After 33 days of working on this nonstop, it seems like I'm starting to get a little bit burnt out and not able to work as quickly. I think I might take tomorrow off to regroup.

Also Monday was a holiday in France, so he said the article should be out some time this Wednesday through Friday.

>>	Anonymous Tue Apr 3 07:51:31 2018 No.9642003 >>9641848 Do take a break if you need it. Meanwhile I'll try to make progress in edit distance calculations, if I have enough time.

>>	Anonymous Tue Apr 3 08:37:51 2018 No.9642053 >>9642003 in Python or something else?

>>	Anonymous Tue Apr 3 08:51:21 2018 No.9642070 >>9642053 In Python since I'm most familliar with it and it already has tons of tools available do do the heavy lifting.

>>	Anonymous Tue Apr 3 10:16:18 2018 No.9642217 Just dropping in to say good job so far anon.

>>	Anonymous Tue Apr 3 14:37:13 2018 No.9642664 >>9641848 you're documenting all this right?

>>	Anonymous Tue Apr 3 23:19:58 2018 No.9643786 have a bump

>>	Anonymous Wed Apr 4 02:51:05 2018 No.9644278 >>9641848 Don't forget to post the link when it's up.

Genetic Eng !neEr...dvM Wed Apr 4 05:45:35 2018 No.9644489

Day 34:

Took a much needed break

>>9642003
>>9642053
>>9642070
Awesome! I really appreciate it.

>>9642217
Thanks anon!

>>9642664
Right now my posts in this thread are serving as documentation, but I probably need to come up with a better system.

>>9644278
Will do!

>>	Anonymous Wed Apr 4 05:51:16 2018 No.9644499 >>9644489 Maybe you could store documentation on the same github repo? When this thread reaches bump limit I'll compile all your daily updates and send them to you in text form just like I did with the previous thread.

>>	Genetic Eng !neEr...dvM Wed Apr 4 06:21:36 2018 No.9644536 >>9644499 Yeah putting them on github might be good. I can probably collect them myself so that I have a chance to edit them and make sure they all look professional.

>>	Anonymous Wed Apr 4 07:56:26 2018 No.9644653 >>9644536 Alse you can put some of the documentation on the project website when it's up.

>>	Anonymous Wed Apr 4 08:59:14 2018 No.9644734 >>9644653 Might also be good for whatever fundraising operation OP wants to do, showing the normies that he is ready to actually put work into it.

>>	Anonymous Wed Apr 4 09:05:25 2018 No.9644747 >>9644734 I think we need to work on this for a while before we can decipher what the fundraiser will be about. How is that Python programming coming along?

>>	Anonymous Wed Apr 4 09:06:27 2018 No.9644750 >>9644734 >>9644747 But overall I think fundraising is a good idea.

>>	Anonymous Wed Apr 4 09:18:45 2018 No.9644763 >>9644747 Already done, submitted a pull request to the project repo with distance tables along with the code and text files, containing protein sequences in case anyone wats to make improvements.

>>	Anonymous Wed Apr 4 10:15:31 2018 No.9644864 >>9644763 good job there

Anonymous Wed Apr 4 14:16:06 2018 No.9645395

>>9644734
A proper documentation would show that he already put in some work so it's necessary.
>>9644750
A fundraising campaign is planned but only after initial preparations are made and whitepaper drafted. If we keep our current pace it should be at most a month and a half before we're ready to launch a crowdfunding venture.

Genetic Eng !neEr...dvM Wed Apr 4 22:40:03 2018 No.9646858

>>9644653
That's true. Also I'm planning to write some more wiki articles.

>>9644734
>>9644747
>>9644750
>>9645395
Fundraising will probably take about a month and a half before we get the money, and then we have to wait for supplies and equipment to ship for another few weeks after that. The sooner we can start the campaign the better, but yes we do also need to do a little more research.

>>9644747
>>9644750
>>9644864
The code looks good! So if I'm interpreting the table correctly, then it looks like EYS is more closely related to perlecan than agrin? Also we may want to try calculating it with a substitution matrix, since some amino acids behave very similarly to each other despite not being exactly identical.
https://en.wikipedia.org/wiki/Substitution_matrix

Anonymous Thu Apr 5 01:45:38 2018 No.9647210

>>9646858
>it looks like EYS is more closely related to perlecan than agrin

It definitely seems so if we look at sequence alignment scores since both Q5T1H1-1 and Q5T1H1-3 have alignment scores greater than 1300, while alignment scores with other protein never climb above 1000. Then again, since I only have a very basic understanding of biology, I don't really know what to infer from this.

Anonymous Thu Apr 5 05:38:43 2018 No.9647608 [DELETED]

Day 35:

EYS is a growth factor first and a matrix protein second.

After seeing the results from >>9644763 >>9647210 (thanks!), I looked into perlecan some more. Apparently in invertebrates, perlecan is expressed as a free-floating growth factor, but in vertebrate animals it has a very large extracellular matrix-binding region tacked onto the end of it. This way when a tissue gets injured, the extracellular matrix will be disrupted and expose perlecan to the surface of the cell, stimulating repair. EYS isoforms 1 and 3 are full-length 350 kDA matrix proteins similar to vertebrate perlecan, while isoforms 2 and 4 are much smaller 65 kDA aqueous growth factors like invertebrate perlecan. So EYS is sort of an evolutionary missing link between vertebrate and invertebrate perlecan.

We should still do more research into it, but I think this is very good for us. We already know that the retinas in patients receiving EYS are damaged, so we probably don't need to worry about producing the larger matrix isoforms of EYS. At just 65 kDA, the growth factor isoforms are well within the range of what we can get to penetrate into the retina (ranibizumab is ~50 kDa and bevacizumab is ~150 kDa). Also since these isoforms aren't matrix binding, we don't have to worry about them prematurely binding to each other or the cellular matrix before they reach the outer segment.

So that was main takeaway from all of the BLAST searches that I did. Also it looks like prominin belongs to the class of integrin-binding proteins, and more interestingly, EYS is related to crumbs-1. I'm still not sure what to make of them being related, but CRB1 is a promoter of cell adhesion while EYS is an antagonist. Also CRB1 is a membrane protein, and I think the two might compete for prominin receptors. I think we need to look into this some more.

Genetic Eng !neEr...dvM Thu Apr 5 05:40:13 2018 No.9647611 [DELETED]

EYS is a growth factor first and a matrix protein second.

After seeing the results from >>9644763 >>9647210 (thanks!), I looked into perlecan some more. Apparently in invertebrates, perlecan is expressed as a free-floating growth factor, but in vertebrate animals it has a very large extracellular matrix-binding region tacked onto the end of it. This way when a tissue gets injured, the extracellular matrix will be disrupted and expose perlecan to the surface of the cell, stimulating repair. EYS isoforms 1 and 3 are full-length 350 kDA matrix proteins similar to vertebrate perlecan, while isoforms 2 and 4 are much smaller 65 kDA aqueous growth factors like invertebrate perlecan. So EYS is sort of an evolutionary missing link between vertebrate and invertebrate perlecan.

We should still do more research into it, but I think this is very good for us. We already know that the retinas in patients receiving EYS are damaged, so we probably don't need to worry about producing the larger matrix isoforms of EYS. At just 65 kDA, the growth factor isoforms are well within the range of what we can get to penetrate into the retina (ranibizumab is ~50 kDa and bevacizumab is ~150 kDa). Also since these isoforms aren't matrix binding, we don't have to worry about them prematurely binding to each other or the cellular matrix before they reach the outer segment.

So that was main takeaway from all of the BLAST searches that I did. Also it looks like prominin belongs to the class of integrin-binding proteins, and more interestingly, EYS is related to crumbs-1. I'm still not sure what to make of them being related, but CRB1 is a promoter of cell adhesion while EYS is an antagonist. Also CRB1 is a membrane protein, and I think the two might compete for prominin receptors. I think we need to look into this some more.

Genetic Eng !neEr...dvM Thu Apr 5 05:41:48 2018 No.9647615

Day 35:

EYS is a growth factor first and a matrix protein second.

After seeing the results from >>9644763 >>9647210 (thanks!), I looked into perlecan some more. Apparently in invertebrates, perlecan is expressed as a free-floating growth factor, but in vertebrate animals it has a very large extracellular matrix-binding region tacked onto the end of it. This way when a tissue gets injured, the extracellular matrix will be disrupted and expose perlecan to the surface of the cell, stimulating repair. EYS isoforms 1 and 3 are full-length 350 kDA matrix proteins similar to vertebrate perlecan, while isoforms 2 and 4 are much smaller 65 kDA aqueous growth factors like invertebrate perlecan. So EYS is sort of an evolutionary missing link between vertebrate and invertebrate perlecan.

We should still do more research into it, but I think this is very good for us. We already know that the retinas in patients receiving EYS are damaged, so we probably don't need to worry about producing the larger matrix isoforms of EYS. At just 65 kDA, the growth factor isoforms are well within the range of what we can get to penetrate into the retina (ranibizumab is ~50 kDa and bevacizumab is ~150 kDa). Also since these isoforms aren't matrix binding, we don't have to worry about them prematurely binding to each other or the cellular matrix before they reach the outer segment.

So that was main takeaway from all of the BLAST searches that I did. Also it looks like prominin belongs to the class of integrin-binding proteins, and more interestingly, EYS is related to crumbs-1. I'm still not sure what to make of them being related, but CRB1 is a promoter of cell adhesion while EYS is an antagonist. Also CRB1 is a membrane protein, and I think the two might compete for prominin receptors. I think we need to look into this some more.

>>	Anonymous Thu Apr 5 08:42:14 2018 No.9647804 >>9647615 Glad to hear that the project is moving forward.

>>	Anonymous Thu Apr 5 11:03:57 2018 No.9648047 >>9647615 Well, lemme know if you need any other programming. I'm primarily a MATLAB faggot, but I like to fuck around with other stuff as well.

Anonymous Thu Apr 5 11:25:40 2018 No.9648088

>>9648047
We also need to make sure that human prominin and zebrafish prominin are compatible >>9630365 by calculating distances between them >>9631251

The principle should be the same so the code already submitted to the repo can be used (with some minor tweaks, of course).

>>	Anonymous Thu Apr 5 14:12:55 2018 No.9648534 Bump.

Anonymous Thu Apr 5 16:50:30 2018 No.9648918

>>9648088
>>9648047
Links to various strains of prominin:
>Human prominin
http://www.uniprot.org/uniprot/O43490
>Zebrafish prominin
http://www.uniprot.org/uniprot/Q9W735
>Fruitfly prominin
http://www.uniprot.org/uniprot/P82295

Though the fruitfly one says that it's a prominin-like protein so I'd like to ask op to confirm if these are indeed the right protein.

Genetic Eng !neEr...dvM Thu Apr 5 18:25:23 2018 No.9649118

Just got a call that they had finished sequencing my mom's sample. Apparently she has the same EYS variants that I do even though she isn't affected by vision problems, so now they're really confused. Either way though, I still have vision loss and I'm still going to keep working on this as a way to repair it.

>>9647804
Yeah, I think with the progress we're making we can start seriously looking at crowdfunding soon.

>>9648047
Will do! Also we might want to try doing some sort of additional analysis to decide whether we want to produce isoform 2 or 4. Both are almost exactly the same size and sequence, but we want to make sure we have the "best" one. I'm not entirely sure what this would entail yet, but maybe we could try computationally folding the individual protein domains and then gluing them all together so that it's a computationally tractable problem size.

>>9648088
>>9648918
Those human and fruit fly sequences are correct, but for zebrafish we probably want prom1b instead. I don't think it will make a huge difference between prom1a and prom1b, but it couldn't hurt to use the exact one.

http://www.uniprot.org/uniprot/Q90WI3

>>	Anonymous Thu Apr 5 18:44:51 2018 No.9649163 >>9618267 you should add something about acquiring basic equipment and access to appropriate facilities to the list of open tasks

Anonymous Thu Apr 5 20:53:07 2018 No.9649501

>>9649118
OP if you manage to make a working cure IMMEDIATELY patent it under some kind of agreement that allows companies to make and sell it but not copyright it, with the possibility of paying you royalties. If you won't some Pharma will copyright your work and make mad cash, and make it difficult for those who need it to access it since they will monopolize the cure and thus raise the price extraordinarily (like that one fag who ramped up the price for aids medicine to 900+$). By using the agreement i mentioned you allow the cure to spread quickly due to capitalistic endevours whilst preventing monopolization of the cure, and as long as you never sell those right you will remain the one who ultimately decides what happens to the product. Capitalist endevours without monopolization are good too, because that drives down prices and makes the cure more accessible.

I look forward to the day when you can cure retinitis pigmentosa 25 (and possibly other forms of blindness) for the price of an eye-drop.

~concerned but hopeful /biz/pol/ fag

Anonymous Thu Apr 5 21:44:26 2018 No.9649625

>>9618267
>We are going to grow it in cell cultures and then inject it, a lot like how diabetics get insulin injections.
Hey OP. Just curious about this and saw you address it here >>9619361
Do you intend to have this as an IV therapy like with albumin/globulins or have it injected intramuscular/subcutaneous/directly into the eye?
Sorry if you brought this up before. I just came across this thread. I rarely come over to this board.
I'm just curious since I worked in an outpatient albumin therapy clinic for a while. Best of luck to you, wish I were able to help out.

>>	Anonymous Fri Apr 6 02:35:55 2018 No.9650208 >>9649501 You make some good points. Placing the whole project on github under some kind of open-source license might also help.

Genetic Eng !neEr...dvM Fri Apr 6 02:56:43 2018 No.9650242
File: 990 KB, 1920x1082, 1430409839968.jpg [View same] [iqdb] [saucenao] [google]

I-I'm not sure what I expected.

https://translate.google.com/translate?sl=auto&tl=en&js=y&prev=_t&hl=en&ie=UTF-8&u=https%3A%2F%2Fmotherboard.vice.com%2Ffr%2Farticle%2F8xk5jp%2Flhomme-qui-voulait-soigner-sa-maladie-genetique-dans-son-garage&edit-text=&act=url

Anonymous Fri Apr 6 03:23:30 2018 No.9650275

>>9650242
Could have been worse. They still exaggerated the DIY side of this and probably told that PhD researcher that you're planning to do the whole thing in your garage instead of just the proof of concept phase and of course the title is clickbait, but that's to be expected.

With all that said, it's still free publicity and might draw more people with more diverse qualifications to the project (that french ophtalomologist seemed supportive of the idea).

>>	Anonymous Fri Apr 6 03:32:56 2018 No.9650282 >>9618373 I like you and hope you suceed

>>	Genetic Eng !neEr...dvM Fri Apr 6 06:11:11 2018 No.9650466 Day 36: The guy from Vice is editing the article before re-releasing it and I'm emailing the ophthalmologist and geneticist to get their feedback.

>>	Anonymous Fri Apr 6 06:15:35 2018 No.9650471 >>9650466 Awesome. How's the whitepaper coming along?

Genetic Eng !neEr...dvM Fri Apr 6 06:25:01 2018 No.9650487

>>9649163
Yeah I think that's a good idea. We're beginning to approaching the experimental phase pretty rapidly, and hopefully we can set up some the equipment while we're still crowdfunding in order to save time.

>>9649501
>>9650208
I don't think a copyright would offer us much protection, but I'm also not sure that we can patent any of this since we're just taking a human gene and growing it in some cell cultures. Still, we should be eligible for a seven year orphan drug exclusivity period, which is almost as good as a patent.

>>9649625
The blood-retina barrier prevents us from administering intravenously, so we have to inject it intravitreally into the eye itself. Feel free to check out the wiki if you want to help out though!

>>9650275
Yeah, at the very least it will be a trial by fire. I'm reaching out to the two people in the article to get their detailed feedback.

>>9650471
Not the best. It needs a lot of rewriting because of all the new stuff we found the last few days. I think we might need to put it on hold until the dust settles.

>>	Anonymous Fri Apr 6 06:32:48 2018 No.9650490 >>9650487 Imo the wiki needs a changelog so that newcomers can see the amount of work already put in. Also reorganizing the code section into folders will make it easier to read. When can we expect the edited article to be up?

>>	Genetic Eng !neEr...dvM Fri Apr 6 06:42:06 2018 No.9650500 >>9650490 Yeah that's a good idea. Also it looks like it's up again: https://motherboard.vice.com/fr/article/8xk5jp/lhomme-qui-voulait-soigner-sa-maladie-genetique-dans-son-garage

Anonymous Fri Apr 6 06:57:38 2018 No.9650512

>>9650500
This version is way better. It explains the project phases and steps necessary while acknowledging that this is no walk in the park or a publicity stunt and doesn't portray you as a biohacker who has no idea what he's doing (like this guy http://www.bbc.com/news/world-us-canada-41990981))

>>	Anonymous Fri Apr 6 07:13:39 2018 No.9650533 >>9650512 yea the article is actually really nice

>>	Anonymous Fri Apr 6 07:22:52 2018 No.9650546 >>9650533 The title is still clickbaity though, but VICE is not subscribtion based so it's understandable.

Anonymous Fri Apr 6 08:50:43 2018 No.9650694

>>9650487
>It needs a lot of rewriting
I think finishing the whitepaper should be one of the top priorities, that way we'll have something to show to bothe experts and professional investors alike. A finished whitepaper would also provide us with a lot of material to use as write-ups for the project site, many anons have already made nice-looking sites, but they still lack content.

>>	Anonymous Fri Apr 6 09:03:13 2018 No.9650717 Can someone tl;dr me as to why we need publicity?

>>	Anonymous Fri Apr 6 09:11:59 2018 No.9650740 >>9650717 Basicly to spread awareness of the planned crowdfunding campaign and reach a wider audience of potential contributors.

>>	Anonymous Fri Apr 6 12:45:47 2018 No.9651326 Bump

>>	Anonymous Fri Apr 6 14:08:14 2018 No.9651578 >>9650740 And we need moniez for?

>>	Anonymous Fri Apr 6 14:32:58 2018 No.9651663 >>9651578 Securing basic equipment and purchasing initial protein samples (500 dollars for 3g of EYS, not counting other reagents).

>>	Anonymous Fri Apr 6 15:16:28 2018 No.9651801 >>9651663 so that's a few k? A fundraising campaign if done properly would pull in millions IMO.

>>	Anonymous Fri Apr 6 15:19:25 2018 No.9651813 >>9651801 OP estimated costs to be at about 15k, but a well planned campaign could well exceed this.

>>	Anonymous Fri Apr 6 16:29:08 2018 No.9651964 >>9651813 I agree. We have to have a good groundwork and all. Then all that's left is a few catchy videos that are on point.

>>	Anonymous Fri Apr 6 17:41:48 2018 No.9652187 File: 210 KB, 1903x928, openeys.netlify.com_ (3).png [View same] [iqdb] [saucenao] [google] Any other webdev anon also still working on a website example? Continued a bit with the version I posted previously. https://openeys.netlify.com/

Genetic Eng !neEr...dvM Fri Apr 6 19:22:06 2018 No.9652363

>>9650512
>>9650533
>>9650546
I agree, it's definitely much more fair. It was nice of him to edit it.

>>9650694
You're definitely right, but we're also not completely done with background research either yet, so writing it is going to be tricky as we learn new things. I think we need to try recruiting more bio anons to help with documentation, since it seems like we're running into a major bottleneck where all of the documentation depends on me.

>>9650717
>>9650740
It came to us, so we took it. Not completely sure yet about how it turned out, but the idea was to help raise funding and technical support.

>>9651578
>>9651663
>>9651801
>>9651813
>>9651964
While we definitely could have a massive crowdfunding campaign, I think it's best to keep a low profile for now until we're further along. We don't want to become solar-freakin-roadways, except for a rare and serious disease. If for some reason we aren't successful, and we have a bunch of desperate RP sufferers give us their life savings, that would be very, very bad.

>>9652187
Looks really good! I can type some stuff up in markdown, and then would you be able to copy it over?

Anonymous Fri Apr 6 19:25:47 2018 No.9652366
File: 51 KB, 516x309, 0.jpg [View same] [iqdb] [saucenao] [google]

>>9652187
We have to make the title more normie-friendly so that they can understand straight away what it's about. Maybe we could someday get some propaganda people to help. Plenty of those in the west these days... yeah, but they're busy. Busy, busy busy...

Anonymous Fri Apr 6 19:53:48 2018 No.9652429

>>9652366
There is no original text yet.
It's either taken from the Whitepaper or other sites from the github repo.
>>9652363
>I can type some stuff up in markdown, and then would you be able to copy it over?
Yes, the site can take the markdown directly from the repo files.
Whenever you or someone else updates some .md file, it can be reflected on the website right away.

>>	Genetic Eng !neEr...dvM Fri Apr 6 19:57:09 2018 No.9652439 >>9652429 >Yes, the site can take the markdown directly from the repo files. Oh wow, that's amazing. Did you roll your own way of doing that? Also how should they be formatted?

Anonymous Fri Apr 6 20:09:53 2018 No.9652462

>>9652439
right now it's fetching the .md, when the whitepaper-site is opened and then parses it to HTML in the browser.
https://github.com/Nocory/OpenEYS-website/blob/master/src/components/whitepaper.vue#L22
Even better would be to do it at build time, so that whenever the repo is updated, the site automatically rebuilds itself with the latest content. Basically what GitHub-Pages does.
>Also how should they be formatted?
Just the way it is right now. The text would render the same on GitHub and on the website, unless you would want to style it differently.
Only for the task list I had to add some extra newlines before the "##" headlines, otherwise the list wouldn't work
https://github.com/Nocory/OpenEYS-website/blob/master/src/components/contribute.vue#L24
Would do a pull request to change it, but that doesn't seem to be possible for wiki entries.

>>	Anonymous Fri Apr 6 21:15:37 2018 No.9652617 http://www.abcam.com/recombinant-human-eys-protein-ab166318.html

>>	Anonymous Sat Apr 7 01:51:21 2018 No.9653222 >>9652439 Did those French researchers answer your emails?

>>	Anonymous Sat Apr 7 02:05:26 2018 No.9653246 File: 16 KB, 323x326, 85283527.jpg [View same] [iqdb] [saucenao] [google] >>9619388 >fda accreditation is easy What the fuck are you smoking? Thats literally the most difficult and expensive part of any new medication or treatment.

Anonymous Sat Apr 7 05:17:38 2018 No.9653444
File: 47 KB, 559x532, Screenshot from 2018-04-07 02:11:50.png [View same] [iqdb] [saucenao] [google]

Day 37:

Did a bit more reading about perlecan and crumbs-1. Also sent the email to the ocular geneticist that I started last night (transcript: https://pastebin.com/NXj7PWyS).). He is clearly very skeptical, and for good reason, but if he can't produce a better technical criticism than "what you're trying is hard", then I think we're doing something right.

I think things are stable enough now that it's time to start seriously considering crowdfunding. Pic related is everything that we need to do in order to get ready. I think we should aim to have all of this done exactly one week from now, and launch the campaign the following week.

I'm going to be focusing on the wiki/whitepaper/website this weekend.

>>	Anonymous Sat Apr 7 05:19:41 2018 No.9653447 >>9653444 I'll try to calculate prominin distances.

>>	Anonymous Sat Apr 7 05:20:44 2018 No.9653448 >>9653444 Pastebin says that page is removed.

Genetic Eng !neEr...dvM Sat Apr 7 05:36:28 2018 No.9653462

>>9653444
Forgot trip. I need to get better at proofreading.

>>9652366
I think the landing will probably be a higher-level overview, while the other pages will have more information. So we'll have both.

>>9652462
My uMatrix was blocking the script so I didn't realize it was doing that at first. Really nice work! Would you prefer to stay the webmaster, or should we transfer it over to github pages? Also I can pay for a domain name.

>>9652617
That's a good find, but it looks like that one is expressed in plants. For our application we need it to be expressed in animal cells, preferably CHO or HEK293, for glycosylation reasons. Do they offer any other versions of EYS, or is that their only product?

>>9653222
The first guy is a pretty influential ocular genetics researcher, so I emailed him, but the second person just looks like a random LASIK surgeon. I decided to hold off on sending it until this morning so that I would have a chance to proofread it. We'll hopefully hear back early next week if he responds.

>>9653246
It will definitely be our biggest time and money expense, but luckily protein replacement therapies and orphan drugs tend to be easier to approve.

>>9653447
Awesome, thank you!

>>9653448
Works on my machine. Make sure you don't copy the trailing paren by accident. https://pastebin.com/NXj7PWyS

>>	Anonymous Sat Apr 7 05:38:37 2018 No.9653465 >>9653462 The link now works, thank you

>>	Anonymous Sat Apr 7 12:51:48 2018 No.9654131 >>9653447 Done with the distances, though the pull request to the repo also adds a few unwanted files that I don't know how to remove since I started using GitHub just for this project. Any tips on how to clean up the PR?

>>	Anonymous Sat Apr 7 15:09:49 2018 No.9654440 I found these resource that might be useful: https://genome.ucsc.edu/ http://gander.wustl.edu/~wilson/dmelgenerecord/index.html https://blast.ncbi.nlm.nih.gov/Blast.cgi

>>	Genetic Eng !neEr...dvM Sat Apr 7 21:47:54 2018 No.9655368 Is anyone opposed to using openeys.org for the website? >>9654131 Awesome! I'll take a look at it and see what the issue is. >>9654440 Yeah, I've been relying on BLAST a lot. It's a really good resource.

>>	Anonymous Sat Apr 7 22:23:41 2018 No.9655436 >>9655368 openeys.org sounds good if we are an organization, which i guess we are?

>>	Anonymous Sun Apr 8 04:24:04 2018 No.9655933 Bump. I'm gonna see if I can help out in any way eventually. I only have basic knowledge of sciences, so I don't think I can do any of the needed tasks you have on your wiki. Good luck to you OP!

>>	Anonymous Sun Apr 8 04:32:43 2018 No.9655950 >>9655933 Same here. I only have some basic programming background and am otherwise an EE. I reckon there will be doable stuff for me eventually as well.

>>	Anonymous Sun Apr 8 04:34:37 2018 No.9655953 300 is bump limit iirc

>>	Anonymous Sun Apr 8 04:59:20 2018 No.9655980 >>9655953 The bump limit is 310 here (board bump and image limits can be found here https://a.4cdn.org/boards.json)) Anyway, op, could you share the traffic data for the github repo? Curious to see if that article created a noticeable spike.

Genetic Eng !neEr...dvM Sun Apr 8 05:25:59 2018 No.9656003

Day 38:

Worked on a bunch of random odds and ends. Distance comparisons on BLAST, a couple wiki articles, some website stuff, and also experimenting with SVG traceovers, We may want to try contacting /gd/.

I'll make a new thread tomorrow.

Genetic Eng !neEr...dvM Sun Apr 8 05:30:53 2018 No.9656007
File: 73 KB, 741x757, Screenshot from 2018-04-08 02:18:05.png [View same] [iqdb] [saucenao] [google]

>>9655933
>>9655950
Check out the wiki! We have both general and EE-specific tasks we need help with.
https://github.com/EyesOpenCure/EyesOpenProject/wiki/List-of-Open-and-Ongoing-Tasks

>>9655980
It only increased traffic negligibly and personally I'm okay with that. I don't think we were really ready to handle a large volume new of people yet.

>>	Anonymous Sun Apr 8 05:42:51 2018 No.9656014 >>9656007 I agree that we shouldn't invite too many people just yet. Now we should bump the thread carefully so op has time to post a link to the new one here. At which time approximately (in your timezone) will the new thread go up

>>	Anonymous Sun Apr 8 13:29:24 2018 No.9656679 >>9656003 contacting /gd/ would be a really good idea. They could do great creating a logo for the project.

>>	Genetic Eng !neEr...dvM Sun Apr 8 17:15:07 2018 No.9657379 >>9656014 I'll make a new thread once this one hits the bump limit. >>9656679 Yeah I'll post there tonight I think.

>>	Anonymous Sun Apr 8 22:03:36 2018 No.9658061 bump

>>	Anonymous Mon Apr 9 00:10:25 2018 No.9658320 >>9656679 /gd/ could also help with infographics and other promotional materials such as advertisement flyers.

>>	Anonymous Mon Apr 9 00:22:00 2018 No.9658348 >>9658320 >>9629954 Advertisements of about the size of a bussiness card to be put wherever you think people might find them. just saying.

>>	Anonymous Mon Apr 9 01:49:33 2018 No.9658526 >>9658348 That's exactly what some anons already did see >>9627265

>>	Genetic Eng !neEr...dvM Mon Apr 9 05:00:38 2018 No.9658786 NEW THREAD >>9658785 NEW THREAD >>9658785 NEW THREAD >>9658785 NEW THREAD >>9658785

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/sci/ - Science & Math