[ 3 / biz / cgl / ck / diy / fa / ic / jp / lit / sci / vr / vt ] [ index / top / reports ] [ become a patron ] [ status ]
2023-11: Warosu is now out of extended maintenance.

/sci/ - Science & Math


View post   

File: 713 KB, 900x675, wet_lab.jpg [View same] [iqdb] [saucenao] [google]
6418110 No.6418110[DELETED]  [Reply] [Original]

/sci/ approved labs / workstations / battlestations thread

>> No.6418120

>>6418110
all that clutter. the chemicals and glassware are practically asking to be knocked over. also is that wood cabinets? it's like youre in the 19th century. i dont even wanna see what the hood looks like

step your game up nigga

>> No.6418125

>>6418110
So fucking messy and cluttered would not work there/10.

Jesus christ clean the fuck up.

>> No.6418145

>>6418110
Looks like some low budget high school bullshit.

>> No.6418151

i like how everyone here has shit to talk and nothing to show for it.

>> No.6418154

>>6418151
i dont take pictures of my lab, mostly because im not a faggot

>> No.6418155

>>6418151
There's not a single empty counter, where do you actually do shit? I'm struggling to understand right now.

>> No.6418345

I wish I could afford a lab to play around in.

>> No.6418416
File: 1.21 MB, 330x248, 1373072739213.gif [View same] [iqdb] [saucenao] [google]
6418416

>>6418345
we all do bro.

>> No.6418428

looks like a normal bio lab to me

>> No.6418497

>>6418151
Well for one:
A. most people on /sci/ don't work in labs
B. the ones who do don't want their advisor to know they visit 4chan.

>> No.6418501

>>6418110
So much fucking clutter, it's unbelievable.
Get yourself a proper chemical storage cabinet or fridge or some shit. This is disgusting.

>> No.6418513

>>6418110
I'd post pics of my lab but I work for the government and I have no idea if that sort of thing would fly or not.

>> No.6418549

cant post pics, but that lab is atrocious.

>> No.6418595

>>6418110
I'm having a mild anxiety attack just thinking about having to work in there.
Please take a picture of the hood. I need to know.

>> No.6418605

>>6418595
>mild anxiety attack
>from a pretty tidy labspace all things considered

>> No.6418608

>>6418605
>tidy
>OP's pic

?????????

>> No.6418611

>>6418605
Look at that fucking rack. I would be embarrassed. Why the hell would you post this?!
>inb4 you say you aren't OP
No one else could possibly support that atrocity.

>> No.6418616

>>6418608
The actual bench space is fine. Someone should put those bottles on the far counter in some secondary containers but that's obviously not working bench space, nobody ever works below the storage shelves.

>> No.6418618

>>6418611
I'm not OP at all, just an anon who doesn't have crippling OCD.

>> No.6418626

>>6418618
At least I'm not a butt faggot.

>> No.6418630

>>6418513
Did you sign a nondisclosure agreement? Does your site require a photo or media pass to to take photos of the premises? If not, it is fine. If so, no.

>> No.6418634

>>6418630
Still no, not OP.

That's a completely normal biology lab, like any other biology lab I've ever been in. I wouldn't blink twice at that lab if I walked past it in the hallway.

I'm honestly not sure you've ever actually worked in a lab that wasn't an undergrad teaching lab if you think that's somehow a mess.

>> No.6418637

>>6418634
>Replying to the wrong guy.

>> No.6418639

>>6418637
my bad

>> No.6418643

>>6418634
Two labs. One shitty R&D lab when I got done with my undergrad (not telling you where because duh) and the one I'm doing my graduate research in.
Now insult me for doing graduate research. Come on, this is fun.

>> No.6418650

>>6418643
I'll buy that you're in graduate school, but not that you're in graduate school for anything related to biology, because if you were you'd know labs like that are the norm.

>> No.6418665

>>6418650
Physical chemistry program. We do do very little (read none past my first year in grad school) synthesis. Still. How doesn't that shelf cause problems? Even IF that is the norm, you can't possibly tell me it doesn't cause problems

>> No.6418682

>>6418665
It really doesn't cause problems, believe me.

Any individual protocol a biologist follows will call for at least one, probably two or three, specific solutions, usually some variant of a buffer. Those all need their own bottles, plus you need bottles to make a new solution if you run out or need to make a new one. It all adds up to a lot of different solutions the researcher needs to keep handy because whatever protocol they're running that day might need them.

You're probably wondering "why not have a lab stock they draw from as needed". Well, that's because it's often the case that keeping solutions sterile is an issue, so having multiple small containers split among researchers is actually a better solution than a single large batch.

Plus, most of that shit is just some very slight variant on plain water when it comes right down to it, or it's harmless stuff like saline or dyes or whatever. Nobody really cares if you knock over a bottle of half molar EDTA unless you get their shoes wet. The actually nasty stuff is kept in smaller bottles in fire-safe cabinets or in dedicated cabinets.

>> No.6418727

>>6418682
Can confirm, Chemist who visits bio labs. They have alot of glassware on shelves. Everyone has their own Agars, Minima Media, Buffer solutions, you name it, its everywhere. Bio labs are very laxed though. Some even allow people to eat and wear shorts inside them (I'm not sure if its really that common though...)

>> No.6418744

>>6418727
>Some even allow people to eat and wear shorts inside them (I'm not sure if its really that common though...)
Depends on the lab and the model system.

I work in a plant lab and I wear shorts all the time. So does pretty much everyone else. I try to at least wear closed-toe shoes, though, and I keep some scrubs in a drawer just in case.

However, the most dangerous chemical I come into contact with on a daily basis is high-proof ethanol. We wear gloves more for the sake of not contaminating our samples than for protecting ourselves from chemical dangers. If I have to work with anything actually toxic, though, I suit up in a full kit.

Labs that work with more dangerous models or who work with actually dangerous reagents have much stricter policies.

>> No.6418743

What are you god-tier work environment rules, /sci/? Mine:

>All bottles labeled with sticky tape (dog eared for easy removal later) that has my name, reagent name, date in permanent marker
>All pens (1 permanent, 1 sharpie, 1 pilot), loops, pipettes, etc. also sticky taped with my name.
>One tip box of each kind, also labeled with tape. Second box opened and put on the shelf when first box starts running low.
>Two flasks with glass beads + jar with ethanol for used beads: When one is used up, put in autoclave, switch to the second meanwhile
>One 1 lt LB bottle, regularly aliquoted into a 50 ml tube. LB is only taken from tube, bottle is opened when 50 ml runs out or when a very large culture is needed. Every morning, check LB for contamination. (frequently used antibiotics media go in fridge)
>H20 aliquoted regularly into 1.8 ml tube for setting up reactions
>If sharing a bench, sticky tape delimits the boundary of my area from the other person's area
>A box of nitrile gloves (for EtBr), plus a box of latex gloves (for everything else) always in the corner
>Razor on shelf to easily cut things like packaging
>70% ethanol bottle for wiping benchtop at the end of every day, if cover sheets like OP's are available, they are changed weekly
>1.2X (use 5 ml per 1 ml DNA) and 6X loading dyes (use 1 ml per 5 ml DNA)
>All samples marked according to parsimonious alphanumeric system keyed and tracked (including location inside freezer box) through Access
>All plasmids stored at -20C, corresponding bacteria stored in -80C
>All experiments have positive/negative controls when remotely possible
>All DNA preps get nanodrop-measured
>Every experiment result is documented by appropriate measurement (gel, spectroscopy, western, etc)
>Experiments recorded in notebook within max 1 work day of completion
>At the end of each day, leave a paper showing the next day's planned experiments
>Always wear gloves
>Always wear labcoat

>> No.6418748

>>6418743
Forgot also:

>plastic beaker, labeled "solid waste", emptied daily, beaker changed monthly
>glass beaker with some bleach at the bottom for liquid waste, emptied and replaced weekly
>Excel worksheets to calculate reaction recipes and master mixes, when making each reaction, tick each reagent when you add it

I'm trying to make a program to calculate optimal master mixes for me, but unfortunately that turned out to be a variant of TSP. Hopefully I can brute force it quickly enough.

>> No.6418746

>>6418743
>All DNA preps get nanodrop-measured
unless you're submitting that shit for sequencing or you have good reason to suspect the DNA is dodgy, you're wasting time nanodropping those, and time is the most expensive reagent in the lab.

>> No.6418781

>>6418746
Well, it shows in detail if I have DNA or not, and also knowing the concentration is nice because I can use exact amounts for downstream applications like restriction digest.

Unless you're saying don't check if you have the DNA at all, Nanodrop seems faster and cheaper (~2 min per sample) than alternatives like agarose gel (~2 min per sample plus ~1 hour to run gel).

>> No.6418795

>>6418781
>Unless you're saying don't check if you have the DNA at all
Yeah, that's what I'm saying.

I mean, for most of the stuff I make DNA preps for, it's large batches of samples for genotyping, and I know how much tissue went into the tube and that I usually have orders of magnitude more DNA from a typical extraction than I'll ever need, so I never really see the need to check. Occasionally it goes weird, but usually I knew it would anyway because I couldn't get enough tissue for that tube or something.

>> No.6418806

>>6418795
Ah. I mostly do cloning. I figure at 2 minutes per sample, and assuming I lose a day if I mistakenly try to eg ligate a bad prep, it's enough for at least one prep in 720 to fail for me to break even.

>> No.6419208

>>6418806
Oh, ok, that makes more sense then.

>> No.6420239
File: 86 KB, 562x750, robotag_demo__messy_bench.jpg [View same] [iqdb] [saucenao] [google]
6420239

cool cool, nice little electronics setup

>> No.6420244

>>6420239
>dat model m
oh baby

>> No.6420245
File: 561 KB, 800x600, P10000501.jpg [View same] [iqdb] [saucenao] [google]
6420245

Here let me just sit this stuff down here for a week or so.

>> No.6420247
File: 101 KB, 640x480, 127129385_4117490e21_z.jpg [View same] [iqdb] [saucenao] [google]
6420247

Anon you don't mind if I just put my stuff up here for a bit do you? cool!

>> No.6420251
File: 112 KB, 427x640, 2287565841_e4db94267b_z.jpg [View same] [iqdb] [saucenao] [google]
6420251

I couldn't find that thing I was looking for. where is it?

>> No.6420532

>>6420251
kill me

>> No.6420614

where are the home lab setups?

>> No.6420620

>>6420614
my lab consists of a single tool box.

>> No.6420813

>>6418428
>looks like a normal bio lab to me
This. Things be everywhere. PROTIP: If this disturbs you, don't look in their fridge.

>> No.6420891

>>6420247
Fuck you

Are those mammalian cell cultures on the window sill? those are at least BL2 you cunt

>> No.6420924

>>6418110
>no engineers have posted ITT
Show us your cubicles

>> No.6420932
File: 48 KB, 631x432, Parabolic_flight.png [View same] [iqdb] [saucenao] [google]
6420932

This will be my lab for a few days this summer. Can't wait.

>> No.6421074

>>6420932
I did that a couple years ago bro. Hypoxia training was bitching fun. Also get to rove around NASA, get to go in the Apollo command section behind the glass, get to go the the underwater iss section. When I went they still had the shuttle training shit up even though shuttles where grounded. Bound to have changed by now.

When you get time to put together your project they have all this bullshit about FOD, but everyone just ignores it.

Also look up scopolamine on vice, yup remember kids when NASA docters offer you scopolamine just say no thank you politely, and proceed to puke your guts out on the most awesome plane ride you will likely ever take.

also whats your experment, we litterly boiled water, it was still cool to see water go from 271°c to steam in two video frames at 50hz

Waters density does some really weird shit when its super heated that much.

>> No.6421133 [DELETED] 
File: 41 KB, 620x433, 1395122823196.jpg [View same] [iqdb] [saucenao] [google]
6421133

>accessing pay-walled research articles without paying
How? This is such a plaguing issue.

>> No.6421237

>>6420932
>Performing this maneuver in an A300
Meh. The FLCS limits pitch angle, so you can't have as long a period of weightlessness, but it also uses a pitch-to-G algorithm for mapping the sidestick position to elevator response. Possibly higher-quality zero-G, but certainly for less time.

>> No.6421412

>>6420932
this intrigues me, but its laughably expensive.

buddy of mine looked into it and said it wasn't that bad. they do have to pay for maintenance in general, fuel for the ride, a custom jump suit you get to keep(asked him if you could skydive with it, he said yes but it wouldnt be ideal), and wages for like half dozen people in the air, + support staff on the ground. so maybe they arent making bank on you, but its still laughably expensive for what and how long it is.

>> No.6421440
File: 145 KB, 1632x1224, 20140318_121749.jpg [View same] [iqdb] [saucenao] [google]
6421440

theoretical physicist reporting in

>pic related, all I need

>> No.6421471

>>6421440
>drinking coffee out of a jar
wow so unconventional
good on you

>> No.6421478

>>6421471
that's not coffee jesus
that's a jar I use for collecting discharge from the machine's washing cycle
I drink from coffee cups