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/sci/ - Science & Math

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2859933 No.2859933 [Reply] [Original]

I'm at lab, on a Saturday afternoon, being bored.

Ask me some questions /sci/

>> No.2859946

why are you browsing 4chan in your lab on the weekend?

>> No.2859950

what is the most dangerous piece of equipment in your lab?

>> No.2859955

>>2859946
Cause I'm doing experiments, but right now that just involves running a gel. And watching a gel run is not fun

>> No.2859960

Undergrad research fun or distracting?

>> No.2859966

Southern, Northern, or Western blot?

Do you have a bigger microscope than me? I've got a pretty damn big one....

>> No.2859970

>>2859960

Both but very impressive for graduate school. I wouldn't be doing research if it didn't look so good on my resume since it's time consuming.

>> No.2859971

>>2859950
I guess our big centrifuge, there's not much dangerous equipment here. As for chemical the ethidium bromide is a carcinogen, paraformaldehyde and pehnol-chloroform:isoamyl alcohol is rather toxic.

>> No.2859987

>>2859960
I'm a graduate student.
>>2859966
We don't do Southern, northern, or westerns in this lab really. Mostly qPCR. Also did you know that only Southern is capitalized because it's someone's last name; western and northern are just take off of the original and thus should not be capitalized unless the first word of a sentence.

And idk if my microscope is bigger than yours. We have a few different ones in the lab. But I'm sure I could get access to EMs and other sorts of things if I tried through the university.

>> No.2859998

>>2859987

> But I'm sure I could get access to EMs and other sorts of things if I tried through the university.

Nice :-).

I knew that northern and western blot were based off of the Southern blot naming system, but I didn't realize that they are not typically capitalized. Thanks for the info.

>> No.2860058

bump

>> No.2860098

I was told recently about southwestern blots. Biologists can be such fucking retards.

>> No.2860104

What's your project?

>> No.2860107

>>2860098
Could you please put this in the form of a question?

>> No.2860119

>Do you have a bigger microscope than me?
>And idk if my microscope is bigger than yours.

I am an engineer and I can't help but giggle at this.

>> No.2860124

>>2859933
are you in college or work?

>> No.2860135

>>2860104
My main project is working with human embryonic stem cells to directedly differentiate them down a glial cell lineage. To understand the cues that make them do this and understand how once they differentiate what allows them to keep cell identity (considering every cell has the same DNA)

There are other side projects, but that's really my main one.

>> No.2860143

>>2860124
graduate school.

>> No.2860147

>>2860143
what time is it? For me it's 23:25

>> No.2860162

>>2860147
Time to go bed, kiddo.

>> No.2860173

>>2860162
you could atleast name your location

>> No.2860182

>>2860147
it's 17:31 here

>> No.2860193

>>2860143
>>2860143
>>2860143


welcome to reality brah.


go to O-Chem floor of your chem dept.


fuckers work 90 hours per week.

>> No.2860203

>>2860193
Just to be clear, I wasn't complaining about the hours I work or that I'm here on a weekend. Nearly everyone in my lab works 10 hour days every day and we're fine with that.

>> No.2860229

>>2860203


10 hours is nothing bro.


even the P-chem people work from 9 am to about 11 pm every day.


they eat on campus in 2x 30 minute sessions.


everyone works at least 6 days per week ("saturday is a mandatory work day"), and at least 50% of the people are working 7 days per week at any given time.


plenty of people spend 1-2 days per week working until 2-3 AM.
Chemistry graduate school is about 50,000,000 times harder and more gruelling than any other form of grad school.


especially biochem and MCDB.

>> No.2860233

>>2860203
Do you consider doing this all your life? I am asking because we have a chem teacher at our school with a docorate and was wondering why someone like him would turn research down and educate us. ((not middle school but chemistry on middle school level (weird business school))

>> No.2860255

>>2860233
>turn research down and educate us
he probably made up that story so as to not make himself look bad

>> No.2860264

>>2860255
what do you mean? He really has doctoral degree in chemistry

>> No.2860273

>>2860255
also i presumed him as a researcher since he has motherfucking dr in chem

>> No.2860277

>>2860229
I don't see why you're trying to argue with me about how difficult things are for my or any other person's graduate experience. When you work with cells and animal models you can only do so much so fast. And what you seemingly perceive as our light work days haven't seemed to hurt us as our lab just had the feature article in a recent issue of Cell Stem Cell and we currently have a paper in review at Nature Methods.

And why do you make your posts in such a weird style

Is it so that people will know that you made them even if you don't have a trip?

It's just kind of weird and annoying

just my opinion though

>> No.2860282

how many RPM is ur fastest centrifuge

>> No.2860307

>>2860282
The fastest one in that is directly in the lab is ~13.3x1000 RPM

The one down the hall goes up to 40x1000 RPM I think. I don't usually have to use it

>> No.2860324

>>2860229
Fuckkkkkkkkk. Damn it. Seriously?

>> No.2860328

OP, what graduate school are you trying to get into? And how enjoyable is working in your lab? I'm asking because I'm a freshman in undergrad who hopes to eventually get into research, specifically either neurobio-related or pharmacology-related.

How exactly did you obtain your research job? Are you paid, and if so, do you mind me asking how much?

Also, are you doing this research to publish a paper or...?

>> No.2860391

>>2860328
Well I thought I already established that I am currently in grad school, but I'll just assume you didn't read the whole thread.

If you want to get into grad school doing research I'd suggest you try and get into a lab ASAP to see if you actually enjoy it and get experience. Grad schools love it when you've actually worked in a lab. My school didn't even interview people unless they had previous lab experience.

In my lab now there is a freshman. I did however do research in my undergrad and I got that job by just looking at all the researchers at my previous school who looked like they were doing interesting research and then just emailing them telling them that I was an undergrad and looking to do research cause I was thinking about grad school.

If you're going to start doing research this early it might be feasible for you to work in more than 1 lab over your undergraduate career, but if you can get on a paper because of it and get decent GRE scores you'll be golden when you start applying.

>> No.2860398

>>2860391
oh, also when I worked as an undergrad I did it for class credit during the year and pay during the summers. I don't remember how much I earned, like $9 or $10 an hour I think.

As a graduate student I get ~2k a month

>> No.2860447

>>2860391
Thanks OP.

Also, What grad school are you in if you don't mind me asking?

>> No.2860454

I'm confused by who is who in this thread.

I'll just ask my questions regardless of if they've been answered.

Do you enjoy working in a lab and do you see yourself doing it for the rest of your life? If so, SPECIFICALLY what do you want to research?

>> No.2860483
File: 25 KB, 1029x678, arpanet3.gif [View same] [iqdb] [saucenao] [google]
2860483

>>2860447
I don't want to tell you exactly because it might be pretty easy to find out the lab that I'm in and who I am.

But I will tell you that the institution I am at is one that was part of the original ARPANET

pic related


>>2860454
I do enjoy working in the lab. I have a lot of fun doing it. I can see myself doing it the rest of my life, but I'm not too sure if I'd prefer to go to industry or academia. If I do stay in research, which I think I will, I think I would like to stay in the general field I am currently in which involves stem cell research although I don't know if I want to work on the exact topic.

>> No.2860489

they are short answer / free response
they are related to quantum theory and periodicty

1. The highest-energy electron in an unexcited atom has quantum numbers of n=3 L=2 m1=1 and ms= +1/2. the electron is the last that can go in the sub shell it is in. The electron is in an atom of what element? Explain

2. Compare and Contrast the elements Antimony (Sb) and Iodine in terms of the following
(a) Electron affinity
(b) Metallic Character

3. Suppose that a stable element with atomic number 119, Symbol Q, has been discovered.
(a) Write the ground state electron configuration for Q, showing only the valence-shell electrons
(b) Would Q be a metal or nonmetal? Explain in terms of electron configuration (I’m guessing covalent bonding is a factor?
(c) What would most likely be the charge of the Q ion in stable ionic compounds?
(d) Write a balanced equation that would represent the reaction of Q with water.

>> No.2860506

>>2860489
Not my area of expertise so any answer I could give would most likely be unsatisfactory.

>> No.2860507

how are memories formed?

>> No.2860527

>>2860506
alrighty then.
any ideas where i could such information?

>> No.2860533

>>2860507
by experiencing things

>> No.2860540

>>2860483
OP

Do you go to Stanford, MIT, Harvard or UCLA? If so, how the FUCK did you get into that specific school? Any tips on getting into top-tier schools like that? Still in undergrad wishing to get into an ivy league.

>> No.2860565

Hey OP, what would happen if you implanted a hESC into a mouse blastocyst?

>> No.2860580
File: 183 KB, 670x881, questions.png [View same] [iqdb] [saucenao] [google]
2860580

>>2860540
No, I don't. I didn't want to go to those schools for grad school because they are farther away than what I wanted to move and the cost of living is way too high and the competition is cut throat. Those are more the type of places I'd want to go as a postdoc, but if you want a good chance at getting into them here are some good tips:

>keep a good GPA
>get good recommendations
>Get lab experience as soon as possible, doesn't really matter what it is or if it is related to what you want to study in grad school (Lab PI can write you a rec letter)
>get published, or at least on some papers
>get good general GRE scores
>Take the Biochemistry, Cellular and Molecular Biology GRE subject test
If you get above 70% percentile on this you've got a really good shot because this is the hardest subject test there is because it's so hard to study for. It's just a bunch of crazy shit that you either know or don't. There's only I think one study guide book that you can buy and it's shit.

http://www.ets.org/Media/Tests/GRE/pdf/BioChem.pdf here is the practice if you want to look at it. I suggest you start studying for it at least a year before you take it.

pic is a page of random questions.


>>2860565
the embryo would probably regulate itself and kill the foreign cell

>> No.2860618

>>2860580
Fucking christ, legit advice. I love you, bro. Thanks you, man. I'll keep all this shit in mind.

I just need to figure out who I can ask to do research & I think I'm golden.

>> No.2860657

>>2860618
If it comes down to it just go to your dept website, or a dept you're interested in and start going down the list and emailing people.

Profs/researchers are very helpful when people are trying to get into a lab. If they dont' have a spot they will usually help connect you with someone who does. And if you're already taking science classes or major classes your profs will probably help.

Protip: they really like it when you tell them that you want to go to grad school to do research, and not med school because so many people that major in a science want to go to med school.

>> No.2860676

>>2860657
What if I don't get publications when I apply?

>> No.2860706

Not OP but I have worked in a chemistry lab for about a year and I am starting in a biology lab next year. Like OP said talk to professors, especially if you have them for class. Many are extremely helpful

>> No.2860752

>>2860657
>>2860706
Fuck yeah, that's a great idea, OP. I am actually going to go and do that. Thank you, good sir.

>> No.2860768

>>2860676
Publications aren't necessary, just helpful, having extensive (1.5+ years) lab experience is much more important. It usually even takes graduate students 1.5-2 years to get their first publication if not longer. It's just like icing on the cake to be on a paper. And I'm sure most people that apply, unless they are people that already have their masters, don't have first author publications.

But if you want to get your PhD in something like the biological sciences it's advisable now a days to just skip getting the masters.

Just email the people you're interested in working with and tell them that you're an undergrad interested in (whatever subject they work on here) and were wondering if they had room for an undergrad to learn some lab techniques for course credit or pay. But if you're serious about wanting to go to grad school give up getting money if you have to cause lab experience is very attractive on an application. It would probably be beneficial in getting in to a lab to tell them that you want to go to grad school. And if you really want to impress them find their most recent paper and read the abstracts and work in the conclusions/finding of them into the email, maybe drop a question or two on them. A good place to find questions is look in the discussion, people always speculate on their findings there and usually propose some questions.

>> No.2860782

>>2860768
You're a damn bro. That's all I can say. <3

>> No.2860804

>>2860782
No problem, but don't procrastinate. You should start emailing people next week.

>> No.2860900 [DELETED] 

bump

>> No.2860913

What's your major?

>> No.2860947

I wouldn't think Utah would be very open to stem cell research.

>> No.2860951

>>2860913
I am a genetics PhD student

>> No.2860984

>>2860947
Why not?

>> No.2861012

>>2860984

Mormons basically run Utah

>> No.2861086

bump