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/sci/ - Science & Math


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File: 183 KB, 468x305, mouseToothLabGrown.png [View same] [iqdb] [saucenao] [google]
15471422 No.15471422 [Reply] [Original]

Can someone point out the flaws with this paper? I looked at the pictures and skimmed through the words, looks like Masamitsu Oshima and co made something funny. All just cope & hope?

QRD,
1 get a pregnant mouse,
2 kill her,
3 steal her unborn baby,
4 cut it's head off,
5 steal it's not-yet-formed molars,
6 extract mesenchymal what-it cells from the molar and separate them from eachother,
7 grow the cells a bit in the lab so they form a clump again (undo what 6 just did)
8 acquire another mouse
9 cut him open, pull his kidney out (gently)
10 open the thin membrane surroudning the kidney forming a little pocket
11 package the clump of tooth-forming cells in a plastic tube
12 put the cump of cells and plastic tube into the mouse kidney
13 sew him and his kideny back up
14 let everything heal
15 wait about 60 days
16 get the now grow mouse tooth from the kidney
17 remove plastic tube from tooth
18 remove mouse's fully functional molar (owie)
19 let it heal over
20 cut the gum open again
21 stick that newly grown mouse tooth in the hole
22 ???
23 profit, mouse now has tooth again!!!! :)

If I had to guess the big problem with this paper is that the original source of tooth forming cells was from a mouse embryo, sadly adult humans probably don't have any of those stem cells in their jaws that we could extract and grow as shown above.

Either that or big-dentist would assassinate anyone who tried.

oh btw, source:
https://pubmed.ncbi.nlm.nih.gov/28361313/

lolpaywall:
https://sci-hub.se/10.1007/978-1-4939-6949-4_8

>> No.15471444

>bro if you just kill a baby mouse, rip its gums out, implant them in a kidney, leu it grow teeth, then jam the teeth into an adult mouse's jaw, you can give mice new teeth!!!
lol at modern bioscientists thinking they're anything more than Mengele with fancier equipment

>> No.15471889

>>15471444

My Wife works with mice models,

Yes, this shit can be gruesome, but it's the only way we get some idea as to what the fuck is actually going on at times.

We know so much, but actually so little about organisms.

trying to cure degenerative disease means we need to study it, and trying random drugs on humans is never going to fly unless it's been tested before on animals.

if we stop this, medical research pretty much stops beyond what can be ethically done on humans, which is a tiny fraction.

>> No.15472472 [DELETED] 

>>15471889
>bro we had to do it
>how would we ever know you can grow mouse teeth in another mouse's kidney without killing and mutilating mice
You guys really are doing important work

>> No.15472538 [DELETED] 

>>15472472
Live with your only set of adult teeth for life then. Give me any hints to what's wrong with the procedure in the paper, and thus why it wont work.

>> No.15472547 [DELETED] 

>>15471889
cutting up mice like they're playdough isn't science cunt, at most you are just playing in a sand pit

>> No.15472583 [DELETED] 

>>15472547
not if he writes down how he made his sandcastle. Besides, they're not your mice and you don't have to cut them up.

>> No.15472593 [DELETED] 

>>15471422
>oh no, mouse experiments exist, le evil science
Grow up faggot
>we can't get those stem cells from human embryos
We can make literally any stem cell, look up induiced pluripotent stem cells

They figured out a protocol to make new teeth. It's pretty expensive, and iPCS technology needs to be a bit more reliable before we use it on humans, but this is an impressive result none the less.

>> No.15472599 [DELETED] 

>>15472538
>Live with your only set of adult teeth for life then

That's what most people do. Dentures are also fairly common and effective.

You could always just encourage greater oral care to preserve teeth.

But none of this involves mutilating helpless animals, so you're not interested.

>> No.15472636 [DELETED] 

>>15472593
>We can make literally any stem cell
Haw can one grow a foreskin? Asking for a pal.

>> No.15472653 [DELETED] 

>>15472636
Enough with the antisemitism, retarded mutilated goylem.

>> No.15472677 [DELETED] 

>>15472593
I wasn't complaining at all in the OP. I want to know why the experiment i linked to might not work on humans. The stuff they did was in 2012, the paper is from 2017. To me that seems a long time ago considering how good looking the results seem.

I want to believe, but I always assume we cant have nice things.

>They figured out a protocol to make new teeth.
Source please. Too good to be true.

>> No.15472680

>>15472599
>You could always just encourage greater oral care to preserve teeth.
Sure, but as we can see that doesn't work in the majority of people.

>But none of this involves mutilating helpless animals, so you're not interested.
Nature exists for man to exploit. Mice don't care if a cat or Dr mengle doing the killing.

>> No.15472682

>>15472593
>look up induiced pluripotent stem cells
Already heard of that, they are supposedly rather limited in potential. Don't know why, hope they aren't. Provide source to give evidence im wrong.

>> No.15472820

>>15472680
>Sure, but as we can see that doesn't work in the majority of people.

If people cannot take care of their first set of teeth, why do they deserve a second?

>> No.15473339

>>15472820
Deserve? Nobody deserve anything you socialist mutt. They work, then they spend their work on buying new teeth. Perhaps in the distant future, with enough mouse blending, we may be able to genetically modify humans to naturally grow a replacement set of teeth in the jaw ready to erupt when needed.

Another excellent use of such research would be to modify cattle to have the same ability, their teeth wear out with use.
Does anyone on this board have any idea about this sort of thing, all i've got so far is environmentalists crying about mice.

>> No.15473400

>>15472472
I would kill a lot of mice for the power to regrow my teeth.

>> No.15473614
File: 272 KB, 952x557, 1675785488874364.png [View same] [iqdb] [saucenao] [google]
15473614

>>15471444
>>15472472
>>15472636

>take 11 patients with clinical condyloma acuminatum (genital warts)
>then take biopsies of warts
>then snap freeze them
>then grind each sample in phosphate-buffered saline with sterile sand, using mortar and pestle
>centrifuge the shit out of it
>take the supernatant and stored at −80°C
>then take a neonatal human foreskin from routine circumcision
>cut it into fragments of 1 by 1 mm
>incubated in 250 μl of the inoculum ( from steps before) for 1 h at 37°C
>implant graft under the skin of ear and under the renal capsule on both sides of three 5- to 8-week-old female mice
>kill the mice after 12 weeks
>none except one of the mice had a renal abnormality
>take the graft from the implant
>split it, fix one part with formalin and snap freeze other part in liquid N
>make histological assesment
>oh hmmm one of three got wierd in the renal area
>must be the Human papiloma virus
>because unsure
>take another neonatal human foreskin prepare it as before
>incubate in 225 μl of the (renal mice) lysate for 1 h at 37°C mice.
>implant it under the renal capsule on both sides of six 5- to 8-week-old female mice
>repeat experiment using a different foreskin on six additional mice
>sacrificed mice 19 weeks later.
>take now 11 of the 12 mice grafts
>grind them up and take again neonatal human foreskin as just as before and inoculate it as before
>but this time 3 by 3mm
>renal grafts are grafted in the usual manner, one per kidney, in six 6-week-old male mice
>repeat the experiment 4 times with different foreskins each time
>if mouse gets sick and has wierd malformation arround the kidney
>then my experiment was working and I have proven the HPV virus causes cancer, by implanting forskin next to mice kidneys
>100% logical and 100% ethical and 100% foreskin
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC110112/

Because of this experiment, girls take the HPV vaccine with the age 12 or so.
>picrel is the mouse with foreskin renal implants

>> No.15473710

>>15471889
>trying to cure degenerative disease means we need to study it

Kek.
No.
Just stop poisning people.
"Live" sciences is a retarded excused to torture animals in absolutely dumb ways.

People literally know that compunds cause degenerative diseases, and excreting them heals them.
Yet nobody cares or investigates on:
>how to remove Phtalate from the body
>how to remove lead, arsenic, antimony, aluminium from bone and tissues
>how to remove dioxines and "medical residues" from the body


But "buy a buckload of animals and torture them in random ways, and then literally come up with nothing new for another 25 years"

>> No.15473754

>>15471422
>japanese names
fraudulent

>> No.15473759

>>15472599
>You could always just encourage greater oral care to preserve teeth.
Not gonna help much if a nigger punches your teeth out for example.

>> No.15473760

>>15471422
>do they Archimedes?
is this a reference to something? seems vaguely familiar but I can't place it

>> No.15473911

Wait until OP hears about auto-transplantation using wisdom teeth

>> No.15473990

>>15473710
Pretty sure the way those compounds are removed is by specific enzymes converting them into some more soluble form. Reason why those compounds are trouble I suspect, is too low a number of those enzymes, due to humans not evolving in environments where theres lots of PbAsAl etc around.

Solution might be to genetically update the body cells with an engineered gene to produce and manage production of an improved enzyme. I'd say we'll need at least 300 mice though.

>> No.15473992

>>15473754
proofs, if you think it wouldn't work, tell me your reasoning.

>>15473759
also won't help if your a kid raised on sugar.

>>15473760
birds

>> No.15474534

>>15471422
I'd feel nervous if I was Bryan Johnson's son.

>> No.15475675
File: 372 KB, 1920x1080, lizz.jpg [View same] [iqdb] [saucenao] [google]
15475675

>>15471422
LMAO, Humans can't even grow new teeth to replace damaged ones, that is how backwards and savages they are!