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11220204 No.11220204 [Reply] [Original]

CRISPR TALENS ZNF

anything. how can I find researchers willing to attempt to treat my condition? or at least talk to me.

>> No.11220214

>>11220204
What is your conditions?
For the moment, CRISPR could be envisioned to treat embryo and a few genetic diseases.
You will have to wait probably 10 years before broader applications might hit the market.

>> No.11220216

>>11220214
human trials are coming: https://www.sciencemag.org/news/2018/09/zapping-mutant-dna-mitochondria-could-treat-major-class-genetic-disease

plus a girl has been cured of sickle cell.

my condition is fluoroquinolone toxicity. i made thread for this before too with no results.

>> No.11220247

>>11220214
>You will have to wait probably 10 years before broader applications might hit the market.
10 years. its always 10 years away. why do we have to wait? people are sick now.

>> No.11220251

>>11220247
Blame putting safety first. Most "cures" arent getting approved because they are too risky

>> No.11220255

>>11220214
In biotech, there is always a massive hype with new technology... until the first problems arise in clinical trials. Then, there is a long delay between technology maturity and application. I think CRISPR will follow the same way...

>my condition is fluoroquinolone toxicity.
CRISPR is for genetic disease mostly. I don't see how it can help you :(

>> No.11220261

>>11220255
because this drug damages the DNA. mtDNA especially.

>> No.11220262

>>11220247
>why do we have to wait

Because we have to:
>check the safety associated with the new technology
>develop the manufacturing of this new technology
>overcome all the unexpected issues
>perform the clinical trials
>answer to people who complain about the time required to develop a drug
and then
>answer to people who complain about the price of new drug

>> No.11220265

>>11220261
Ok... but if the damage is different in every cells, then CRISPR cannot fix it :'(

>> No.11220269

>>11220261
And? You want to use CRISPR to cut it up near every place where it was destroyed then HR it up?

>> No.11220271

>>11220265
i dont know if it is.

https://www.hindawi.com/journals/omcl/2017/8023935/

this is a overview of what the problems might be but i cant understand much of this. im not saying you should read it because its long but if someone could and tell me what theoretical treatment can cure me, id be grateful.

>> No.11220275

>>11220271
Kurwa. This is my uni

>> No.11220279

>>11220275
really? please, do you think you could reach out to the authors of this paper or something? they havent replied to my email. I am desperately looking for something besides just supplements. im looking into stem cells too but reading mixed things.

>> No.11220283

>>11220279
sorry that was too forward. im just really at a loss right now

>> No.11220284

>>11220279
Anon, i will be frank with you. After skimming through this paper, given how this thing works, CRISPR wont cure you, as they said, it shreds mtDNA, CRISPR cant cure that one.

>> No.11220288

>>11220284
so what can, if anything?

>> No.11220295

>>11220288
please

>> No.11220298

>>11220284
is there some experimental cell repair research that might do it? stem cells seem like they help some people but not as much as id thought

>> No.11220300

>>11220288
Dunno man. They outlined three possible causes of FQ toxicity. Destroyed mtDNA is one of them, second is disrupted gene expression (which they think is "best", as in easiest, to treat), third makes mitochondria perpetually "leak" energy. m we dont know which is true yet. In the end this research group doesnt have an answer(figures, the guy from my uni is crazy for mitochondria in general, not curing dieseases)

>> No.11220307

>>11220300
thanks for dumbing it down.

>Destroyed mtDNA is one of them
>third makes mitochondria perpetually "leak" energy.
why wouldnt embryonic stem cells cure it then? cant you just place the cells with the damaged mtDNA with the new cells and you're good?

> second is disrupted gene expression (which they think is "best", as in easiest, to treat),
then why not CRISPR/TALENS/ZNF for this?

>> No.11220310

>>11220271
I briefly read the paper.
The damage to mtDNA is probably not regular and cannot be treated with CRISPR.
In addition, it seems to induce epigenetic changes, which is not treated with CRISPR.

How to cure your symptoms! Sadly, I have no idea.
Maybe, you can search more about Urolithin A. It seem to replace mitochondria. But I don't know more I have no ideas if it can help.

https://en.wikipedia.org/wiki/Urolithin_A

I hope your conditions will improves.

>> No.11220315

>>11220204
You already made this thread, fuck off you sack of shit.

>> No.11220317

>>11220315
you fuck off if you dont like it.

>> No.11220321

>>11220307
Mitochondria exists within a cell. Also they consider this possibility, later in the paper. They want to stimulate mitochondria to proliferate(in the cell), so the healthiest one will be the most abundant. Problem with stem cells is, the cells with bad mitochondria exist RIGHT NOW, stem cells would have to replace entire thing and this takes a pretty long time(though this isnt my area of research)

CRISPR has a problem, it needs around twenty nucleotides long sequence that it has to recognise, then it cuts. Anything else is an addition. Now why is it bad? Because CRISPR works badly in differentiated cells, it isnt global(we dont know how to make it global), there are offtargets for the sequence you cut. The paper we talk about postulates that FQ creates some sort of feedback loop and we need to stop them.

>> No.11220326

>>11220321
>Problem with stem cells is, the cells with bad mitochondria exist RIGHT NOW, stem cells would have to replace entire thing and this takes a pretty long time(though this isnt my area of research)
Yes, so why not just brute-force replace every single bad cell with fresh ESCs?

>stem cells would have to replace entire thing and this takes a pretty long time
im guessing this would cost an absurd amount of money. but theoretically, from my basic understanding, i think it would work wouldnt it?

>> No.11220328

>>11220326
but of course, that feedback loop would continue. even if that worked, i guess the mtdna would gradually start shredded again because of the epigenetic changes and stuff?

>> No.11220346

>>11220326
This is sadly where my knowledge ends, anon. We are moving away from my area of knowledge, but the thing you think about is asking for cancer

>> No.11220351

>>11220328
You are mistaking what happens tissue wide with what happens in individual cells. Epigenetic changed are cofined to one cell(and eventually progenitors), they cant jump from cell to cell(unless of course the FQ persists)

>> No.11220359

>>11220351
oh ok I see, thats good then. im waiting to hear back from stem cell treatment clinic to see what they can do for me. ok I need to get this question answered then. whether its just a matter of cost/time. id risk cancer honestly to possibly escape living like this. i cant even stand, been stuck in bed.

>> No.11220366

>>11220359
because literally, replace damaged cells with good cells that have healthy mtdna. dont really see the problem. there has to be something im missing or else everyone suffering from this would have done it. if its a question of risk, ill take it.

>> No.11220371

>>11220366
If it was only about the risk, someone would attempt it. Sadly, as i said, my knowledge as of why is lacking.

>> No.11220372

>>11220359
>im waiting to hear back from stem cell treatment clinic to see what they can do for me
They can do nothing for you.
Stem cells are a scam.

The best think you can do, eat as healthy as possible (lot of fruit and vegetables). Probably your best option right now.
Try to do some exercise if you can.

>> No.11220373

>>11220372
they're not a scam, im talking to people who have gotten some results. im just wondering why the results arent even better.

>> No.11220377

>>11220372
>Try to do some exercise if you can.
i cant stand without risking further damage

>> No.11220382

>>11220366
Though. If FQ isnt metabolised and stays there, stem cells wont help. Also the paper you two've been talking about mentions that cell replacement in muscles and brain is very slow. This is why stem cells wont help

>> No.11220389

>>11220382
>This process, however, cannot take place in the central nervous system and muscles because the cell exchange is close to zero in these tissues. Also, the collagen exchange is very low causing the tendon regeneration to be a difficult and long-lasting problem.
Exact quote just in case

>> No.11220391

>>11220373
>im just wondering why the results arent even better.
Because, apart from the placebo effect, stem cells do nothing.

>> No.11220398

>>11220216
just dont take drugs then?? LOL


what a no life loser

>> No.11220401

>>11220398
It was prescribed for a sinus infection

>> No.11220413

>>11220389
Damn. I don't know then. I'm fucked for life most likely. My life as I knew it is over

>> No.11220416

I just don't know.

>> No.11220418

Nobody will help you for the next 10 years or so, but you can kill yourself and stream it.

>> No.11220421

>>11220377
>i cant stand
You have nothing! You are just LAZY!
Just move our ass OUT of your fucking bed!

LOL

>> No.11220426

>>11220418
I don't think I'll stream it.

>> No.11220429

>>11220310
thanks for the thought

>> No.11220432

>>11220413
>My life as I knew it is over
How old are you?
Since when are you in this condition?

>> No.11220437

>>11220429
At the other hand, anon the paper proposes some small solutions.
>Cyclosporine A and metformin are postulated to be able to close PTPs [102, 103] and protect against OS [102, 104–106]; thus, it is an interesting substance for possible FQAD treatment.
>Lowes et al. [79] show that the mitochondrion-targeted antioxidant MitoQ protects against fluoroquinolone-induced oxidative stress and mitochondrial membrane damage in human Achilles tendon cells. In cells treated with MitoQ, the oxidative stress was lower and mitochondrial membrane potential was maintained.
>The substance that is postulated to possess the ability to promote the mitochondrial biogenesis is pyrroloquinoline quinone (PQQ) [107, 108]. This substance is also postulated to be OS protective [109].
>Thus, ozone therapy can be examined to be a method of FQ degradation in the body.

>> No.11220438

>>11220382
>Also the paper you two've been talking about mentions that cell replacement in muscles and brain is very slow
even when directly injecting?

>> No.11220441

>>11220437
im taking PQQ and CoQ10 right now and its not helping. or maybe its making it better than it would have been otherwise. ill look into the other stuff but seems like cope with no way to directly address the problems. im fucked

>> No.11220449

>>11220438
Sadly, you cant cheat that one.

>> No.11220460

>>11220449
i dont even know then anon.

>> No.11220481

>>11220437
is there IV PQQ? ill look

>> No.11220485

>>11220441
>im fucked
Again.

How old are you? Since when do you have this condition?

>> No.11220497

>>11220485
mid 20s. its been a week and my body is declining daily. before you say im jumping to conclusions or its too early, im not. ive read a lot. this is very likely going to be a years-long , probably lifelong process. i might be lucky and feel 90% better in a year like a few people but i wont count on it. i know im also suffering from the negative mental effects but this seems to be the case objectively from all the case studies im reading and studies.

>> No.11220515

>>11220216
Dont take fluoroquinolones then bruh

>> No.11220523

>>11220515
got a time machine I could use?

>> No.11220531

what are your symptoms?

>> No.11220563

>>11220497
>its been a week and my body is declining daily
My hangover are longer...

Have you been diagnosed by a doctor?
Again, look into Urolithin A:
https://actu.epfl.ch/news/compound-with-anti-aging-effects-passes-human-tr-2/

Also, you need a diet rich in anti-oxidant. Which fruits and vegetables... and no more cheetos!
Maybe a few vitamin supplements will not hurt for a 2-3 weeks.
Also, try to think about something else and move a bit.

>> No.11220590

>>11220563
>Again, look into Urolithin A:
ill order some.
>Also, you need a diet rich in anti-oxidant. Which fruits and vegetables... and no more cheetos!
i shit you not, im actually allergic to most fruits. but ill try. im more of a meat and dairy guy though. and i dont eat chips.
>Maybe a few vitamin supplements will not hurt for a 2-3 weeks.
already on it a couple for recovery.
>Have you been diagnosed by a doctor?
most doctors know little to nothing about this unfortunately. i will probably find a neurologist or something to assess the damage though.

>Also, try to think about something else and move a bit.
im stuck in bed for now but ill try.

>> No.11220593

>>11220590
>neurologist or something to assess the damage though.
and someone to look at my body too.

>> No.11220603

>>11220590
>most doctors know little to nothing about this unfortunately.
Still, you should consult a doctor! Do this before asking advice on internet....

>and someone to look at my body too.
A prostitute could probably relaese the symptoms for a short period of time.

>> No.11220611

>>11220603
>A prostitute could probably relaese the symptoms for a short period of time.
good joke but i couldnt do that if I wanted in my current state

>> No.11220616

>>11220531
OP is just obsessed with CRISPR, probably a pretty young man

>> No.11220627

>>11220616
i really dont give a shit what helps, ive already been convinced ITT that crispr isnt it

>> No.11220776

>>11220247
Check these
> thalidomide catastrophe
> sulfanilomide elixir catastrophe
> aids blood donation
> fmt deaths
It takes so long because letting people die is infinitely better than hooking up half the world on some shit to discover crippling side effects lasting for generations when it is too late.
You let out CRISPR out, and next thing you know all young parents want to give their children big blue eyes. Unfortunately the blue eye mutations used in retail applications make collagen more fragile, and by the age of 30 all such people are on life support. Since everyone has blue eyes, everyone dies. How's that for a scenario?
When you play with drug approval, you are always playing with a bad genie. Such orgs are one of the few things govmt is useful for. Too bad it can misuse this sacred power, as in Sackler morphine drug. Oxycontene or smt like that. Hopefully they are trying to clear their sins, but the damage is already done

>> No.11220784

>>11220247
Because there's too little knowledge and because nobody could afford it anyway.