/sci/ - Science & Math

>>9815594
>not wearing a oversized wu-tang clan tshirt
Yep, sorry kiddo I'm not doing business with you

kek, acually contribute to science rather than slitting peoples' wrists instead of your own.

>>9815647
Are you talking about yourself, neet? Because that sounds like projecting.

Biohacking may be the most retarded expression I've ever heard. Articles on them always seem to feature some semi-fat dude who takes 10 pills each day for no visible effect aside from whatever placebo satisfaction he derives.

I'm the guy on /biz/ looking at growing bootleg hormones for the consumer market. Mostly I'd be doing it to fund private research of my own.

>>9815670
My advice is get your Wozniak mr. Jobs, if you catch my drift.

>>9815694

You mean a Tesla to go with my Edison?

>>9815909

>>9815594
I'd rather do actual biology in a laboratory

>>9815967
Are you unemployed or just poor?

>pipetter above 90 degrees
i know there's nothing in it but i'm still cringing

>>9815594
How long until biohacking can be used to cure aging?

>Biohacking
>Neckbeards and goths sticking magnets and LEDs under their skin
>We're cyborgs lol

>>9816270
Why the common mistake to confuse cyborgs with biohacking? The cyborg community even has his own name (grinders).

>>9816271

Transhumanists who prefer the notion of becoming cyborgs are the loudest part of that movement. Inexplicably this has altered people's perception of anything remotely transhumanist in nature.

>>9816270
This.
Biohacking is a fad, nothing more.

Biomedical Engineering — the field as an entirety — is what will lead to actual advancements. The “biohackers” are just people who weren’t popular in high school and need to do something to feel special.

>>9816680

When I first read that "biohacking" was a thing, I assumed it was going to be people doing mad science out of their houses with commercially purchased equipment. Then I read up on it. Turns out it's mostly just people doing the same things that every "health and nutrition" magazine has been discussing for ages, it's just replaced "health and nutrition" with "biohacking" because that sounds cooler to millennials.

>>9815594
Hey guys, I'm pretty well-read and intelligent (M.D. in Bioengineering) though I've been wondering this a lot; if blood causes skin to grow because it turns into scabs which turns into skins, then why don't we make blood that turns into skins so we can bleed and literally re-skin things? I mean I don't think it'd do much though it'd be cool because we can.

>>9816813
I know this is an obvious troll since you can't get an MD in bioengineering (MD stands for medical doctorate, at least troll correctly and say PhD), but I'll bite anyway. Skin doesn't form from blood, it forms from keratinocytes and other skin cells. Scabs are formed from clotting proteins in blood and platelets that cause the stoppage of a wound to prevent further bleeding.

My head hurts from your post.

>>9815631
Well you're in luck because hes IS wearing an undersized wu tang shirt

>>9816813
Wut

>>9817021
That's the whole fucking problem
He makes it look gay as fuck
Pic related
This is how you should wear wu tshirt

>>9815594
This is the genius who’s taking his own crispr supplements, right?

>>9815594
Because biohacking is a meme popsci term, I think you mean "genetic engineering", the prerequisite for that is actually understanding your field.

>>9815970
>Nu/sci/ LARPing as if he actually understands science.
Do you know how much money it costs to set up and then run a laboratory?

>>9816040
Soon just you wait for SENS™.

>>9817177
Not him but not much if you do most things diy
Maybe like 2k max

>>9817183
>How to kill yourself the expensive and painful way.
Sure thing, buddy.
I don't think you've mastered enough glass blowing for that, although I'm sure you're great at another sort.

>>9816271
>naming yourself after an app only used by the grossest of fags

>>9817177
>>9817188
Hi LARPer, I bet you are unemployed.

>>9817044
this is infact wrong... you dont have to be a stuck in an underground mid 1990s man child aestetic to rock the Wu-Wear...

>>9817214
Just you know, when you display a certain lack of knowledge that give us any confidence of your credentials and THEN attempt a reversal of this nature.
It simply doesn't work.
Look, if you want to know more about the field, it might help if you tried checking out the sticky.
Alright, n00b?

>>9817238
Fuck, so you are unemployed, lmao.

>>9817183
consumables is where you get fucked. you can cobble together replacements for most lab equipment that are... adequate, if not ideal. consumables tho will eat up all your money quickly.

essential equipment, emphasizing reusability
- manual defrost freezer 150 bucks
- pressure cooker 100 bucks
- used pipetter 100 bucks
- used glass petri dishes - 35-50 bucks for set of 12
- glassware - 5-10 bucks for a 500 ml pyrex erlenmeyer, 20 bucks for a 1L flask. could easily spend a few hundred bucks getting a basic set
- used microcentrifuge - 80-100 bucks will get you an ok one
- thermal cycler - 300-500 bucks for a used modern unit
- electrophoresis rig - minimum 100 bucks for a power supply with a high enough output voltage. if you can get your own platinum wire you can make your own gel tank, otherwise expect to spend 80-150 bucks on the tank

the manual defrost freezer is for bacterial stock and competent cell storage, pressure cooker to repalce an autoclave. there's plans out there for replacement centrifuges that use like, bicycle wheels, but used benchtop centrifuges are cheap enough you shouldn't bother. glass petri plates are a bigger upfront cost but cheaper in the long run. you can get around pipetters for some larger volumes but you'll need some way to reliably measure small volumes no matter what. You could probably cut cost on the thermal cycler with an arduino-based DIY platform

some reusables
- kanamycin - 50 bucks for 50 grams (basically lifetime supply, just using this as a proxy price point for whatever selective agents you'll need)
- tips - 30-50 bucks per 5-pack
- reusable large-volume syringes, 5-10 bucks a pop (to replace large-volume pieptters)
- standard molbio chemicals like tris (25/100g), edta (25/100g), etc.
- agarose - 30/50g . you'll go through 50 grams pretty quickly.

two grand would probably get you the start of a home lab but you're cutting corners at every step

>>9817243
Nice retort, almost as solid as >>9817214, kudos.

>>9815970
Poor, for now anyway. But, my work isn't reckless or irresponsible. I'd sooner build my success off using myself as a test subject than dupe some idiots; that would have worse long-term consequences.

>>9816809
Oh is that what it is? I thought it was like, that asshole who injected himself with his CRISPR kit or something

>>9817261

Mostly that's what it is. The guys using themselves as guinea pigs are an extreme outlier.

>>9817261
>>9817319
Go read the articles instead of assuming things. Is not hard.

>>9817249
That's some interesting points about reusables. If you don't have money you can arrange a local institution to borrow his lab to support a startup and make money by creating and providing processed chemicals for lab use. Is a nice way to make some profit.

Could you theoretically clone dinosaurs with this?
Asking for a friend.

>>9815594
>mfw just set up two monitors for my computer, opne for each eye
how much of a biohacker does this make me?

>>9817374
Mosquitoes in amber don't have that much DNA, John Hammond.

>>9815594
isn't he dead?

>>9818916
Are you the same shitposter from the other thread?

>>9818919
i dunno, isnt he dead?

https://www.youtube.com/watch?v=QcPtzqgoXt8
>yfw people are already making money with biohacking

>>9815594
Because I don't want to end up dead in a sensory deprivation tank

>>9815594
I've tracked down a useful enzyme that would make the chemical production of a major fine chemical obsolete. The source organism was sequenced with Sanger and lacks curation. Also the feedstock chemical is damn obscure and not a side product of any useful chemical process. So I'd have to track the encoding gene for that too. Hours of FRET and sequencing for what? Thats assuming the fucking enzyme folds right when expressed in whatever cheap-ass organism I shove the gene into. The metabolic engineering for the feedstock chemical production is a necessity as well... Fuck that noise, I decided to screw it and play music.

So how hard would it be to ghetto produce HGH today?

If people can make glow in the dark yeast at home they could just as easily make yeast or bacteria that produce the hormone, correct?


We might actually be seeing affordable an decent quality UGL HGH soon.

>>9816813
>I'm pretty well-read
wrong
>... intelligent
wrong
>...MD in-
wrong
>Blood = liquid skin
Fucking summerfags

>>9821316
you're dumb for not calling him out on having an "MD", you get a masters for that focus.