/sci/ - Science & Math

http://www.the-odin.com/genetic-engineering-home-lab-kit/
Get a DIY crispr kit now.

Injecting into the eyeball.

CBS News will be airing a 1hr documentary "Playing God" tomorrow (wednesday) about crispr including a piece about diy.

Seems like knocking out myostatin inhibitors could have untold negative effects, the most likely/obvious of which is cancer.

I'd give it about 98% chance that nothing happens and 2% chance that he gives himself cancer.

>>9282664
Whilst he's probably a con artist and if not likely stupid - i do appreciate people who are willing to put themselves on the line in order to advance science and medicine.

>>9282780
Long before that your heart becomes a solid mass of scar tissue

>>9282664
>“This will modify my muscle genes to give me bigger muscles,”
fucking lol for some reason

>>9282671
Time?

>>9282664
Some tech CEO woman already did this to increase her telomerase or something. Enjoy your cancer

>>9282808
This. But still interesting.

what virus did he use to replicate the edit to all his cells?

>>9282664
Might as well use steroids at least you'll know the side effects.

>>9282664
why are biology types so often nu-males?

>>9283143
Nature is a woman. Both nature and women select which males will pass on their genes. Plus, nature is what "gives birth" to you, physically.

Nu-males exist because they try to get closer to women by not competing in the sexual market. Biology types are similar. They go to biology because they feel a connection with nature.

Another way of saying this is that, because nature is feminine, it takes femininity to appreciate nature.

>>9283153
Go back to r3ddit faggot

>>9283153
I agree, nature is a bitch.

>>9283162
Back to r3ddit!

>>9283143
How is jamming a needle with untested gene edits for muscle growth nu male?
Its fucking stupid but not weak

>>9283177
>memes
>tatoos
>that hair
>that body

The reason he's putting himself in danger is not "bravery". He just wants fame, he just wants to be recognized as "brave" in the easiest possible way.

>>9283188
I mean hey, he can have fame, what he's doing is at least interesting (if also pretty dumb). Maybe some decent public knowledge will come out of it?

http://www.the-odin.com/human-myostatin-knock-out-targeting-crispr-cas9-plasmid/
>just a Cas9 expression vector with a single guide RNA
jesus christ this will do literally nothing when injected.

He's gonna die RIP

>biohacking

He also did a poop transplant with vice. I seriously doubt that there was even anything wrong with him in the first place.

>>9283188
I've seen vidya youtubers look less faggy

>>9283291
Go back to r3ddit

>>9283007
Lol at u

>>9282664
Didn't we already have a solution for that called anabolic steroids? I don't get it, do people just want to use the word "gene" for some reason while re-solving solved problems? Is this the "quantum" pseud popsci craze equivalent for biology?

>>9283228
>http://www.the-odin.com/human-myostatin-knock-out-targeting-crispr-cas9-plasmid/

>This product is not injectable or meant for direct human use. The DNA needs to replicated and purified before use in human cells.

>>9282664

You laugh now, but just wait six weeks...

>>9282997
he's a phd

The startup sells home lab kids to run some basic CRISPR experiments. Overall pretty cool.

>>9282664
If I looked like that I'd want to change my dna too.

They're selling the plasmid here with instructions
http://www.the-odin.com/human-myostatin-knock-out-targeting-crispr-cas9-plasmid/

#BIOHACKYOSELF

Tell me about CRIPSY
Is it just a meme, or will we actually see """superhumans"""

>>9284328
>will we actually see """superhumans"""
>Implying we haven't already seen them

>>9284337
>fucked a ladyboy and had his heart explode from a hot tub

next showing of playing god on cbsnews is in 1 hr 45 minutes. I caught the end of the last showing and it was actually semi-decent.

https://www.cbsnews.com/live/

Playing God Video On Demand links
https://www.cbsnews.com/live/video/20171109005930-this-new-technology-could-change-human-evolution-as-we-know-it/

https://www.cbsnews.com/live/video/20171109012905-are-we-entering-an-age-where-we-can-create-our-own-designer-babies/

https://www.cbsnews.com/live/video/20171109012952-what-are-the-pros-cons-of-crispr-technology/

>>9284341
His one weakness...

i can clearly see his motivation for biohacking...

>>9282668
That’s not a bad price. Isn’t that about what thermal cyclers go for on their own?

I would do it if I had something like type-b lymphoma. Yescarta will costs you 350,000+ and your other option is to gamble on 7% response with chemo. Why not DIY yescarta with what you got?

>>9282664
too bad it wont fix his atrocious fuckken face

>>9284161
>The DNA needs to replicated and purified before use in human cells.
that still won't make it work if he just injects the plasmid directly into his bloodstream.

>>9284799
>Isn’t that about what thermal cyclers go for on their own?
he's probably hoping to fly under the radar, thermocyclers are expensive because of the licensing fees.

>I would do it if I had something like type-b lymphoma
sure, if you're in a huge bind, try whatever the fuck, but just injecting plasmids like he's doing isn't going to do shit. you need to get it into the nucleus of the stem cells that make the tissue in question. for a lot of tissues, that is not a trivial problem, and in practice it means using some kind of viral vector (which he isn't selling).

a viral vector delivering a genome-altering plasmid in a DIY setting? that's a recipe for a horribly painful death, not a cure.

>>9284176
Bane?

>>9284197
Oooh a PhD? Clearly genius then.

>>9285492
I mean, I don't like playing the appeal to authority game, but if he got a PhD from U Chicago he's clearly not book dumb, it's an extremely selective program (I should know, I got weeded out after the interview.) That doesn't mean he's wise enough to not make really bad choices. Thomas Migdley was a brilliant chemist but he gave himself lead poisoning in a stupid publicity stunt:

>"Midgley took it upon himself to hold a demonstration where he illustrated just how harmless he believed lead to be. He did this by pouring tetra-ethyl lead over his hands and then holding a cup of it under his nose and inhaling it for sixty seconds. Whilst performing this deadly demonstration he was assuring reporters of how he could repeat this practice daily without harm. He did this whilst knowing full-well the dangers of lead poisoning due to having been over-exposed to the substance a few months previous to the demonstration."

>>9285508
>>9285492
My problem isn't that he has a PhD, it's that he has a PhD in biochemistry. I glanced over the few papers he's first author on. None of his papers are particularly relevant to gene editing or human disease; he came from an optogenetics lab and all three of the papers are about studying certain structural features of proteins used in optogenetics experiments. He also is listed as a low-tier author on a couple other papers; he probably generated some constructs they used or did a couple experiments for them, the papers don't look like they're super related to his primary publications.

If I was going to take advice from a PhD on DIY gene therapy, I'd want someone with a PhD who came from a lab studying something related to gene therapy - preferably a lab that is involved in translational research and/or clinical trials, although I'd accept someone who worked in a lab that studied the virus used as a carrier for your transgene and its interactions with mammalian immune systems. I'd also want that person to have personal experience with human cell culture with those same systems.

>>9284149
Yes it is. Like all his genetics in every cell is just gonna be like alright I'll change.

>>9285401
I just read more. He uses PEI Polyethylenimine 10:1 with the dna

>>9283188
>https://en.wikipedia.org/wiki/Signalling_theory

>>9285904
ehhhhhh. Yeah I guess that would work. Still think i'ts a bad idea though.

>>9285946
Not really. It's less risky than most activities. What do you think the odds are for a bad outcome?

>>9286014
Off target editing is a reality for all but a small minority of CRISPRS, and even though CRISPRS are the cleanest editing tool we have, doing it in a scattershot approach like he is increases the risk of chromosomal rearrangements. The standard for gene therapies with CRISPR is to do them in cultured cells so that you can confirm only the edits you desired occurred.

>>9286039
Actually that might be dangerous too. Better to not do it at all. Something could always go wrong and the human body is otherwise perfect.

>>9285909
that makes sense

>>9282664
>Not a vascular injection with AAV carrying a myostatin CRISPR library driven by ACTA1 promoter.
Not gonna make it.

>>9282664

How long is it expected to take in order for him to see results? (if there are going to be any)

>boy lacks ability to keep his skin on due to genetic issue
>the epidermis on 80% of his body simply falls off, and he's about to die as the raw flesh becomes infected
>doctors take a skin sample, genetically modify it, and then grow vast amounts and graft it onto him
>it has now spread to fully cover the bare spots, heals and feels normal, and the child is now indistinguishable from anyone else
https://www.nature.com/articles/nature24487
I fucking love genetic engineering.

>>9282664
that's a nice pr stunt. based on his sad face in pic related i'm thinking it was cyanide or something. He probably want's to die.

most people are like 'CRISPR CAS9 IS FUTUTRE 11111!!'

What they dont realize is the hardest part of this:

finding the guide RNA to go into the gene and remove it.

find the guide RNA that can remove proteins of blood cells?

universal blood that can be given to ANYONE

nobel prize in 1-3 categories

>>9286489
Is there a way to read the whole paper without paying?

>>9282664
Is CRISPR cas9 the future or is it all just hype? I have heard from a lot of people how it can greatly help us and I am doing biology so I want to know how much of a feat it is so I can choose a career focusing on it.

I have Josiah as a friend on Facebook, his stuff is pretty interesting desu but I am not sure if all of it is just hype or if it is actually a decent revolution.

>>9286611
>in biology
>not sure if crispr is a big thing

What the fuck is wrong with you?

>>9286611
Imagine if you are a in a diffrent field, I'm going to go with chemisty.

For years we've known that the electron is the driving force of all of chemisty, however while certain elements have certain electrons, and we can study those electrons, we have kinda hit the brick wall.

Then someone finds something, it allows for the targeted shift in elections from element to element and potentally even cross elements.

Suddenly the world opens, and a new age starts.

In seriousness though here's just a short list of possibilities this tech allows.

-the cure for malaria
-genetic disorder eradication
-enhanced genetics, no longer a lottery
-potentally fix organ/blood donors
-gives us a new chance against the growing numbers of superbugs

That is me being unoriginal and not even innovative. This will probably change the future cyberpunk humans from replacing their bodys with chrome to sleek, rippled fresh.

Even if this particular solution fails, we have a working model, we can improve or change the solution if needed, but this is probably just as significant as the double slit experiment.

>>9283007
https://www.youtube.com/watch?v=S6UqgjaBt4w

Rapture is here

Where the fuck can I get some gorilla gametes? No, nigger gametes wont work, quit asking.

I already have a surrogate to carry the hybrid to term.

>>9286526
>What they dont realize is the hardest part of this:
>finding the guide RNA to go into the gene and remove it.
That's not the hardest part. That's the easiest part. It's so trivial a literal child can do it.

>>9286611
It's a tool that allows us to target proteins to almost every location in the genome at will. From a basic science standpoint, it is the most significant advancement in basic lab methods since PCR. From a translational or clinical standpoint, it's the culmination of decades of gene targeting research and it replaces several tools that are garbage with one that Just Werks.

Think of it like giving a power drill to someone who has spent their entire life drilling holes by hitting a pointy spike with a hammer, or giving an automobile to someone who had to walk twenty miles to get to work.

>>9286762
Harambe is gone, stop trying dude. He will live forever in our memory.

>>9283153
>studied biology to explore the physical components of philosophic cognition, the nature of self, and the perception of choice

>every other biofag is unable to do much more than regurgitate shit they've memorized and then mistaken for critical thought when a soapbox presents itself for some polarizing agenda

>tfw

>will modify my muscle genes to give me bigger muscles

Screw bigger muscles. Give me super flexible muscles.
One step closer to genetically engineered catgirls!!

>>9286584
https://sci-hub.cc/https://www.nature.com/articles/nature24487#
Use sci-hub for such things

>>9287314
The only good post itt