/sci/ - Science & Math

Good luck man.

It's the last written test you'll ever take.

lol is it multiple choice

think of it this way: this is literally the last test you will ever have to take seriously.

unless you decide to go back to school. but as they say, you're only stupid enough to go for your doctorate once.

>>3149377
no

where are you from OP?

Good luck, Dr. Anon

Gook luck, Anon.

This is it:
>The Final Countdown
>http://www.youtube.com/watch?v=9jK-NcRmVcw

PhD in what field?

get you some adderall, OP

>>3149462
genetics

>PhD in Genetics
>any jeans i want
>?? starting

> takehomeexam.jpg

wtf?

post problems

Good luck, OP.

Though you really should probably get the fuck off of 4chan.

>>3149813

>XY starting

>>3149825
>>3149825
>>3149825
>>3149825
>>3149825
this

Good luck anon!

What happens if you fail?

>>3149845
You contract AIDS and die.

>>3149466

Everyone suggests this, but it isn't as easy at everyone makes it seem.

>>3149845
if i fail then I have to take an oral exam either with every prof or with the ones I do poorly on. If I fail that I'm asked to leave the program

>>3149825
Here is one.

You identify a gene on chromosome 12 that upon stimulation with drug X mediates direct conversion of fibroblasts to neurons. You now want to know how this gene is regulated, and so you map H3K4me1 sites, which correlate with the location of gene enhancer elements, by ChIP-seq in unstimulated fibroblasts. The ChIP-seq results reveal no less than 2500 regions that have high H3K4me1 signal on chromosome 12. You now need to figure out which one(s) actually regulate your gene. Describe how you would approach this problem, list the issues that you will need to consider, discuss how to address them, and present how you would prioritize candidate enhancer sequences for functional testing. You have unlimited amounts of money, access to the latest genomics technologies, and a bioinformatician at your disposal. However, your competitor has made a similar discovery and is about ready to scoop you, so you need to design a set of experiments that can be completed in a reasonable time frame to get you your answer, so knocking out the putative enhancers one-by-one in mice is not an option.

>>3149859

It depends on who you know. A lot of people with prescriptions sell it when they reach college to make money. Though, for obvious reasons, I would advise you not to buy from any undergrads from classes you TA (though I imagine if you're taking a candidacy test you're probably done with that).

>>3149875
what the fuck am i reading?

>>3149891

PHD

>>3149875
>Watch every season of CSI
>Replicate episode where lab miraculously compares the hair of an albino to the murder suspect using a rare genetic allele

>>3149891
real science?

>scary isnt it? Back to philosophy and atheism threads for you

>>3149875
is there a tl;dr version of this?

>>3149875

the answer is 42

>>3149875
Sounds like you need essentially a good sorting algorithm and various test procedures that'll lump your regulators.

>>3149875
just finish the test and find some way for me to become immortal.

>>3149913

>The ChIP-seq results reveal no less than 2500 regions that have high H3K4me1 signal on chromosome 12. You now need to figure out which one(s) actually regulate your gene.

How do?

>>3149937
magic. I'm not going to post my answers before the tests are graded

>>3149929

I lumped your mom's regulators last night.

>>3149929

>hurr durr, i got an A in high school biology, means i know what im talking about

>>3149941

bugger you

>>3149951
eh? You got all that from a general analysis of the problem?

>hurr I never learned basic logic and reasoning

yfw grading professor frequents /sci/, sees posted question and gets the wrong impression

>>3149937 You identify a gene on chromosome 12 that upon stimulation with drug X mediates direct conversion of fibroblasts to neurons. You now want to know how this gene is regulated, and so you map H3K4me1 sites, which correlate with the location of gene enhancer elements, by ChIP-seq in unstimulated fibroblasts. The ChIP-seq results reveal no less than 2500 regions that have high H3K4me1 signal on chromosome 12. You now need to figure out which one(s) actually regulate your gene. Describe how you would approach this problem ...

Woah, real problem. Okay let's see. ChIP just tells us which regions bind. 2500 regions is really freaking huge so you can probably rule out regions that aren't directly UTR of your gene (guess: order 100 sites left)...

>>3149969

Oh wait, would it work to just do the sequencing under Drug X condition and not Drug X conditions? Assuming that methylation is causing silencing via these histones... the difference between the two signals would be a good first order approximation for what the drug is doing. Is it that simple?

>>3149984
if so, you'd have a good idea about what signal is cis/trans regulating your gene (categorize that way). I'm not sold that methylation is the only way... I'd prefer to know what the mode of action of the drug is. But the question's about epigenetic regulation.

Follow up experiment is then one or two knockouts of promising regions.

Thanks, OP this helps me understand why I care about binding.

>>3149984
OP here fyi methylation doesn't always silence. H3K4me1 is the mark of an active enhancer and we're talking about enhancers so they are orientation, distance, and location independent.

but you're on the right track

>>3149984
>>3149969
Careful guiz, you might make /sci/ think real science is complicated

>>3149875
Wow, I actually understand this, which I guess I should since I'll probably be that bioinformatician in the question. Best of luck, OP.
Also, lol. >You have unlimited amounts of money,

>>3150008 fyi methylation doesn't always silence.

They keep telling me that, but I'm not buying it. Makes my head hurt. You want me to believe that some spooky binding far away from the TSS of my gene is causing stuff to happen? refs/textbook?

>>3150020
At the end of it, make sure you demonstrate how you would patent the detection sequence and sue your nemisis when he discovers the same method independantly

Also, you'll donate half of your riches to the school.

>>3150008

OP OP OP, I have a question. How does histone modification relate to methylation at CpG sites? At all?

>>3149948

D'HOHOHOHOHO!

>>3149381
I thought anyone mad enough to charge into a doctorate will still have to write scientific research materials on random bullshit he studies.
You know, writing the stuff you find on JSTOR or PubMed rather than using it for once.

As much as I am thrilled to have actual science being discussed on /sci/, I'm gonna say that OP really should work on finishing his test so he can advance to candidacy. After that you can fuck around some, so long as you get sufficiently good at hiding from your adviser.

>>3150094
shhh. we're trying really hard to distract OP. Plus they're probably a woman. Genetics is a womanly field.

>27 hours? Man, I better get on 4chan and tell everyone about it.

OH SHIT, NIGGER, WHAT THE FUCK ARE YOU DOING?

Get back to work, son. What are you going to tell your friends and colleagues? Missed a deadline because of 4chan?

>>3149875
>You have unlimited amounts of money
Pay off someone else to do it.

Also be sure to mention how you'll donate half your money to the school you're taking this test from. Also hope you get an advisor to busy with his own shit to bother you.

>>3150094
>>3150114
Both of these. Back to work, bitch. *whiplash*
ALSO - take a pic of yourself after you pass the exam and put it as a >mfw
We'll remember ya.

>>3150121
One last thing.

Eye of the Tiger and Good fucking luck!

>Implying /sci/ isn't a valid source for scientific research.

>>3150114

>implying anyone can work for 27 hours straight

taking breaks are recommended maybe even a nap

At last someone from /sci/ is about to graduate.

>>3150196
If OP is anything like me he probably slept for ridiculous amounts of time one or two days before this test. He should be okay.

>>3150105
This fired my curiosity. OP are you a woman? If OP is a woman, should she tits or GTFO?

>>3150238
i'm not

good luck op! for the remaining hours just play this song in a loop , or just save it for the very last moments
http://www.youtube.com/watch?v=jLJLyneZGKc

>>3150256
I got a better song for you OP


http://www.youtube.com/watch?v=8bEMWL_8SlU

i didn't know you could get a doctorate in genetics.
sci/ can only math apparently

>>3149875
>You have unlimited amounts of money, access to the latest genomics technologies, and a bioinformatician at your disposal.

Here's the real problem.

OP, would I be fucking myself if I chose Genetics over Biochemistry for grad school? Or is there enough overlap that I can be OK with it?

>>3149875

>>3150238

This is not /b/, we don't do that here. Further, this is a safe for work board and posting nude images can get you banned.

I know it's summer, but please keep the /b/ stuff out of /sci/, it's not appreciated.

>>3150306
Relax, I only meant that as a joke, and I'm really curious about the likelihood of a woman that's a candidate for Phd lurking in 4chan.

Anyways, I just saw a trap pic at the other thread.

OP will undoubtedly fail.


27 hours straight work is stupid.

>>3150333
well fuck you I only have 2 questions left!

>>3150336


gj op that's the spirit
go fail harder nerd

>>3150336
How many questions? And are they all essay questions?

>>3150341
yes they're all essay and I have to answer 10 of 13

>>3149875
>direct conversion of fibroblasts to neurons

this is terrifying.

>>3150386
no, it's real life

http://www.nature.com/nature/journal/vnfv/ncurrent/full/nature10202.html

>>3150415
Yes but that implies I can turn my skin into neurons. I suppose it would have an application in treating leprosy possibly. Then again I don't know anything.