/sci/ - Science & Math

Anyone here have any experience with Crispr Cas 9 and implementing the luciferase producing gene into plants? I have recently started on this project and I'd love if any anons could point me to any sources or perhaps have any tips of research they may have done themselves. Thanks in advance, kind /sci/entists!

Why are geneticists so quiet about the race iq controversy? There's presumably at least some genetic component considering how geographically isolated populations were for tens of thousands of years.

how can I get an internship with a sum of stipent, anons?

>>11459311
Genes do not work the way most people think they work. There are 30000 genes and most of them interact with most of the others. It is very difficult to analyze such a system. The traditional way of analyzing has failed (although there were a few successes early on because of low hanging fruits). The silence is mostly because geneticists know very little, much less than what the general public thinks they know

>>11459311
we meet again dungus.

>>11459370
>we meet again dungus.

>>11459370
>>11459373
both of you. get out pls, i dont this to happen like last time

>>11459375
tell that to cockopoopoo

>>11459378
again, why do people rationalize IQ

>>11459390
black people IQ*

>>11459392
>>>/pol/
do it on another discussion of IQ, but not on a discussion of genes pls, thx - OP

>>11459339
so evolution is complex?
>>11459398
ok then, sorry for disturbance.

>>11459405
ok then, sorry. i thought you were somebody trying to make a discussion of politics.

Bump!

>>11459406
you have to admit, the genes are different in races.

>>11459517
yeah but what should we do? kill em? CRISPR should do the work, dungus, please man

>>11459518
>yeah but what should we do? kill em? CRISPR should do the work, dungus, please man
CRISPR should be banned to white race though, it does nothing but promote harm, cockopoopoo, please man

>>11459521
>CRISPR should be banned to white race though, it does nothing but promote harm, cockopoopoo, please man
why? its extremely useful, are you an anti-vaxx?

>>11459523
>why? its extremely useful, are you an anti-vaxx?
anti-vaxx = anti-gemo, yes sure makes sense.

>>11459526
>anti-vaxx = anti-gemo, yes sure makes sense.
yes, genetic modifcation could enchance any baby. make us finally eridicate cancer and so on

>>11459531
>yes, genetic modifcation could enchance any baby. make us finally eridicate cancer and so on
dude, we're living in reality, not fantasy land where the best stuff happen, show me evidence of CRIPR's progress. please

>>11459533
>dude, we're living in reality, not fantasy land where the best stuff happen, show me evidence of CRIPR's progress. please
dude, visit this: https://www.nature.com/subjects/crispr-cas-systems

>>11459552
jesus christ, the spam system was being a dick to me for some reason

>>11459266
I'd just like to interject for a moment. What you're referring to as 'a gene called CRISPR-Cas9',
is in fact, just Cas9, or as I've recently taken to calling it, the CRISPR/Cas9 editing system.
CRISPR is not an editing complex unto itself, but rather another repeated component
of a fully functioning viral-DNA cleavage system made useful by the CRISPR-associated proteins, guide RNA and tracrRNA comprising a full double strand cleavage mechanism as defined by Zhang et al.

>>11459269
I use CRISPR/Cas9 in Arabidopsis, what do you have questions about?

>>11460476
Thanks man!

>>11459311
Because it doesn’t actually matter to daily life.

>>11459311
Taking an upper division genetics course at a top 20 university. The professors literally said intelligence is mostly heritable, and said it really fast and never mentioned it again, before beginning a lecture on the most commonly methods to find these correlations with genes. I'm under the impression that real geneticists know more than they really want to know and might have their own opinions but just care about keeping their jobs focusing on their own research, so they don't delve into forbidden topics.

>>11462125
true, it's annoying in the age of censorship.

>>11462355
>censorship
get out sad fag, censorship is done to protect you from retarded question which retarded powerful people might believe.

>>11459266
Genetics are just a bunch of pseudoscience, no one is still sure what are genes, just the basic tip of the iceberg

>>11459339
how do you think genes work?

>>11459266
does CRISPR-GEMO work?

What exactly are recessive genes? least likely gene to appear? what if we bred 2 recessive genes together?
Will recessive genes always stay recessive whenever breeding with anyone else?

>>11462726
yes, as long as there's a gene, they will stay recessive when breeding with anyone else

>>11462576
>no one is sure what are genes, genetics is false!
>no one is sure what is gravity, physics false!

>>11462576
>no one is sure what are genes, genetics is false!
>no one is sure what is gravity, physics false!
choose one

>>11462974
mate, there's difference between small branch in big branch, and big branch

>>11463364
you still get the point.

>>11459266
Anyone, wanna discuss about genetics please?

>>11459311
THIS IS WHY

https://www.sciencealert.com/father-of-dna-james-watson-stripped-of-accolades-in-ugly-racism-row

>>11463516
lmao, fags being triggered and ignoring instead of discussing it.

>>11463524
isn't it a bit problematic? like many people will say 'black people are low IQ' which means they should deported and then stolen from again and yeah you get the point, also genes are really subjective.

>>11459266
Why do disabled people exist? Like how does nature allow them to exist?

>>11463574
because nature also allows these stuff to exist

>>11462599
in a complex way

>>11463559
well, black people should contribute to society or else they're useless and harmful

how will genetic editing contribute to anything? it should be illegal and stay that way, there are probably better methods of making the world a better place

>>11463559
>genes are subjective

>>11461353
oh pretty interesting anon! I am looking for ways to implement the Luciferin-Regenerating Enzyme (LRE) into bacteria. I was personally thinking of E. Coli possibly, or perhaps V. fischeri. This way a biological light source could be created which has many ways it can be used. Do you know whether this idea is viable? Or perhaps any suggestions for the bacteria I can use :)

Thanks in advance anon!

bump

>>11459266
is everyone not interested in genetics or what? explain to me.

>>11459266
*silence*

>>11459266
you can all tell me if GEMO works, please

>>11459266
why is no one reading this thread? just tell me you fags?

>>11459266
stop bumping this shitty thread, fag. it's some lowfag 2nd grade explaination

>>11462125
genetics is a really complex subject to study.

>>11464937
how is it complex?

>>11464940
its literally chemistry and math and biology mixed together

>>11464944
well, yes but that doesnt make it complex, it's really easy.

>>11464957
>genetics ez
do you know what it is about? molecules and maths, with biological stuff.

>>11464963
i dont understand

>>11459552
>>11459556
>links articles
they're all exgaggerated

>>11464977
do you think that genetic engineering could end sexism?
>>11464865

>>11464977
>they're all exgaggerated
no they aren't, GEMO will still happen regardless, like the old days when people thought vaccines were dumb crap that didn't do shit

>>11464983
>no they aren't, GEMO will still happen regardless, like the old days when people thought vaccines were dumb crap that didn't do shit
GEMO is way different, vaccines have been proven, where has GEMO been proven? not to mention, its not as dangerous as GEMO, because vaccines are just putting stuff in your body while GEMO is changing the 'blueprints' of your body.

>>11464992
>GEMO is way different, vaccines have been proven, where has GEMO been proven? not to mention, its not as dangerous as GEMO, because vaccines are just putting stuff in your body while GEMO is changing the 'blueprints' of your body.
look at this, https://www.sciencedaily.com/releases/2019/07/190711105621.htm, this is revolutionary dude! changing the 'blueprints' of your body isn't that bad, it may seem bad now. just like vaccines and injecting 'stuff' into your body. GEMO can do good shit too, you hate trannies? you can use GEMO to remove gender dyphoria! hooray

>>11465013
holy actual fucking shit this works, i cant believe it

>>11465013
>>11465019
what do you think? https://www.dw.com/en/crispr-cas9-babies-likely-to-die-earlier-berkeley-study-says/a-49025884

>>11459266
Is dna turing complete tho

>>11465035
>what do you think? https://www.dw.com/en/crispr-cas9-babies-likely-to-die-earlier-berkeley-study-says/a-49025884
same thing with vaccines retard

>>11463842
transforming E. coli cells is relatively easy, using either chemical- or electrocompetent cells

all you need to do is:
>clone the LRE gene into a suitable E. coli expression plasmid backbone
>transform the cells with your plasmid
>select for colonies expressing your plasmid & thus the LRE gene product

however this isnt really a genetics project, and falls more under the domain of molecular biology

>>11465054
no, it still has some problems.

>>11465132
>same thing with vaccines retard
it has the ability to more dangerous though

>>11459523
>its extremely useful, why shouldn't we use it?
Because we're modifying, flattening and computerising a core part of our organism without anything like full understanding of the impact. Like those CRISPR babies could have a defect now, that we'd have no idea of knowing what it was, or how it might emerge generations down the line, but, those CRISPR babies can reproduce with non-modified people extending and "infecting" the greater population with the result of that computerised flattening and mod introduced into their "genetic code" (which looks like a computer code, but isn't.)

>>11465404
>Because we're modifying, flattening and computerising a core part of our organism without anything like full understanding of the impact. Like those CRISPR babies could have a defect now, that we'd have no idea of knowing what it was, or how it might emerge generations down the line, but, those CRISPR babies can reproduce with non-modified people extending and "infecting" the greater population with the result of that computerised flattening and mod introduced into their "genetic code" (which looks like a computer code, but isn't.)
We can keep them monitered, it's not like they will go and do anything they want without us noticing it.

>>11463582
>implying that being these type of species is horrible and not for humans
mate, this is the best type of species any creature could wish for

>>11465518
>We can keep them monitered, it's not like they will go and do anything they want without us noticing it.
sure, let's do the same thing with illegal vaccines... oh wait.

>>11465708
>sure, let's do the same thing with illegal vaccines... oh wait.
retarded fail

>>11465913
>retarded fail
make arguments faggot

>>11465191
thank you, anon! although I do suppose you're right in that this isn't a genetics project.
May I ask how you use/have used CRISPR/Cas9 in your work/projects?

>>11465191
thanks, anon! Although I do suppose you're right in the fact that this isn't really a genetics related questions specifically. May I ask how you use/have used CRISPR/Cas9 in your own works/projects? I've only really recently gotten interested in this stuff and I'd love to hear about some other projects!

>>11466439
>>11466445
I use the CRISPR to generate plants with deletions in a certain family of redundant metabolic enzymes

These deletions will help to better understand the signaling properties of distinct metabolites within a biosynthetic pathway

>mfw all my emails are getting replied with a short rejection emails.
how am I supposed to get accepted in a lab as an intern, anons? nobody's looking for an intern according to emails that I've received.

>>11466470
Can you summarize your emails?

>>11466644
mostly like picrel, anon. i'm talking about my interests and what i can do for their lab. i'm writing a couple of words about their papers. idk what to do anymore. it's like 20th or something like that and i lost my interest for this summer already. i'll be finishing my second year this semester btw.

>>11462125
You're lying because if you're taking upper division genetics you should know how heritability works.

>>11463559
He made dumb, unscientific statements, and one lab redacted some honorary title. What a tragedy.

>>11466755
Countless others spout anti-scientific retardation on the daily and get NEW honorary titles for it, instead of getting anything retracted.
Likewise, countless people got real titles out of bullshit work that isn't even suitable for middle schoolers, let alone fucking doctors.

Bump

>>11463605
>I don't like it so it should be illegal

>>11467595
>I don't think it's dangerous so it should be legal

>>11459266
stroz 2016 - Causes of molecular convergence
and parallelism in protein evolution

what do you think about the probability of fixation as it applies to the model in box2?

>>11459266
here is box two. previous posts image was background info on how the terms convergent and parallel are used in the paper

>>11459266
my opinion on CRISPR/Cas9: I use it in my research and how i use it, it is not any worse than having deformities. Before crispr/cas9 there was RNAi. Also, TALENS has been around as a zinc finger binding element as well. the ability to target a very specific site is the appeal of CRISPR/cas9 system. its also very far away from being used to add genetic material effectively. it can be use to target breaks to destroy gene function very effectively. one of the reasons CRISPR/Cas9 fails to work on a per gene basis is because of the concept of pleiotropy or how a single gene contributes to building an organism in many ways. this is contrasted by the defunct model of one gene one trait.

>>11459266
What effect does incest have on genes?

>>11467958
not OP, the concept behind inbreeding effects of incest is that it increases probabilities of homozygotic rare recessive alleles. homozygote means the same allele is found in your mother and your father and you now have two copies because your dad and mom are siblings and were carriers for the rare recessive alleles. Rare recessive alleles usually exist at a small frequency in healthy populations (make what you will of the jewish gene pool) and are therefore rarely expressed twice in the same organism. so when you ask what sort of effect does incest have on genes? the answer is that it increases the likelihood of low functioning genes showing up together. the effect incest has on the phenotype, or organisms develop/ed/ing body, is that it is usually retarded.

>>11467972
thanks

>>11464980
Yes but that wouldnt be a homo sapiens.

>>11467972
>muh incest is wrong because genes
i had sex with cousin when she was 17 years old, I was 19, we had a kid together and the kid wasn't as bad as everybody said, here's our baby

>>11468267
circumcise him

>>11468388
wtf

>>11468267
it's not your baby exactly, but he has a similar face and a similar skin color, just darker.

>>11468470
our*

>>11468267
dude, don't reproduce again please.

>>11468736
dont give a shit, just because you said. we be reproducin 3 kids after, wat u gonna do bro?

>>11468742
what.... the.... fuck....?? Jesus Christ!

Why do these threads always turn into a race bait? /pol/tards need to be lined against a wall and shot.

>>11468267
poster you responded to here.
i didnt make a value judgement. jews and muslims do it a lot. incest isnt wrong because of genes. incest is wrong because how would you like to be the fucked up retard in your family? it only INCREASES the LIKELIHOOD if parents are already carriers for the same bad alleles. this likelihood increases as you move from distant family members to siblings. your child might be fine, or in a few years when it is supposed to be speaking, you find out both you and your cousin rolled the dice and it came out bad because you were playing with weighted dice. i hope your little baby does fine, homeboy. itd be nice to not have more retards you know?

also, post pic of cousin, faget

>>11469189
ok

>>11470021
so post pic

>>11468742
>that time when someone failed to not reply to a bait post and it polluted the gene pool so bad humanity would eventually go extinct in the far future
What did you do, Anon?

>>11470665
note: that's not how she looks, but she looks exactly like her. ok bro? i aint sending my pic of cousin, you got to know my cousin aint wantin that shit, she aint a fan of being on da internet

>>11471134
nice

>>11470665
before u tell me to send my cousin's pic, i wont nigga, no mater what u goin say

>>11466722
don't worry anon, keep trying. you will get in soon

>>11471279

genes are just sophisticated stuff