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>> No.10527322 [View]
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10527322

I was given a project by my professor to test over-saturation in UV-Vis and Fluorescent spectroscopy using fluorescein. The lab paper said that fluorescien was water soluble. I found out the way that it slightly soluble and was absorbing all wavelengths. I know I need to use a solvent. But which give less overlap?
If I use PH=9 NaOH will that cause an over saturation of sodium ions? Will it affect my absorbance significantly?
Should I use ethanol?
Which solvent would be best for fluorescein?

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