/sci/ - Science & Math

>>16072588
Sweet Jesus I hope you're memeing

>>15947959

>>15938883
I feel sorry for you by this point. You've once again cited something as a "gotcha" that has no relevance to you whatever except your superficial understanding of certain terms.
>digitonin-treated cells
You might want to google what that means.
>The covalently-attached NLS was functional in the digitonin-treated cell system but was unable to enhance DNA nuclear entry in living cells after microinjection.
Here's a free hint.
Oh, and did you wonder what streptavidin was?
>streptavidin-NLS/NL
Might want to look that one up too. With the nonfunctional variant only 1% of HeLa cells expressed any GFP and there's considerable room for error at that low expression with such a high volume injection from simple degradation. The paper does point out degredation though mainly against being able to explain the only example of strong GFP expression (which is a fair point at 10% but definitely not at 1% weak expression) given the methods in 1999.

While we're at it beyond the fact the paper's conclusion notes fairly low success of diffusion and lower expression, though decent given 1999 that was something to be happy about, did you stop to think about the quantities injected?
>220 ng/μl -1.75 μg/μl DNA
lol
Do you happen to know the cellular mass of HeLa cells? I'm guessing no. I do not see reference of specifics and this paper is fairly old, so going with an average I pulled up 2.29ng/cell and some more recent estimates still around that at 2.3 ng.

So, since I know you're completely clueless, I figured I'd give you a ratio to realize just how fucking absurd your level of reaching is here. The live cell 200 ng injection was ~87 times as much mass as the mass of the cell. Ignoring error degradation etc, that achieved 1% GFP expression in live HeLa without streptavidin. So with junk. Requiring nearly 87 times the mass of the cell with direct injection.

And you think this paper is relevant to anything or somehow supports your point? pic rel

>>15935733
>>15935735