/sci/ - Science & Math

Moving around dem genes every day.

bump

If I'm looking for a novel gene (uncharacterized in a particular organism), and someone has already done the work for me (>9000 ESTs on ncbi), how do I search for homologous regions on model organisms (that have known function), to try and characterize the one I'm looking for?

In other words: Huge EST database for this organism, how do I analyze it, barring BLASTing every one individually?

>>3734301
I track my modifications with GFP errday. Think about the usefulness:

>insert green fluorescent protein in same regulatory sequence
>if promoter is allowing expression of GFP, your gene of interest is in there too

It makes keeping track of complex physiological changes so easy, you can just SEE it.