/sci/ - Science & Math

>>15442424
>most of science is a big lie
mot of science is not even scientific.
They just write hypothetical stuff based on a thing in a petri dish, which resembles nothing that exists in the real world.
And sometimes they also torture animals to prove a point

>>15314368
I requested a Isolated purified virus.
You present:
>Mouse-adapted A/PR/8/34 (PR8) was grown in 10–11 day-old-chicken eggs at 33°C for 60–72 h.
Also the "origin" of the alleged "PR8-Virus" is not mentioned, nor verified, that this crap is indeed a virus.

Nor did they talk about the Medium in which the """virus""" is transported.
But since you probably don't know what "purified" and "isolated" means:
>it means nothing but the virus
>or at least suspended in a non toxic/contaminated solution such as:
>destilled water, saline
Since it is in the USA: "Southern Research Institute, Birmingham, AL, USA"
And by requirement of the CDC the transport medium of the virus is contaminated with:
>Sterile, heat-inactivated fetal bovine serum (FBS)
>Gentamicin sulfate (50mg/mL) (or similar antibiotic at an appropriate
concentration to prevent bacterial contamination and growth)
>Amphotericin B (250µg/mL) (Fungizone)

https://www.cdc.gov/coronavirus/2019-ncov/downloads/Viral-Transport-Medium.pdf

Then it is contaminated with egg.
"The supernatant was snap-frozen and stored at −80°C. On the day of inoculation, virus dilutions were made in phosphate buffered saline (PBS)"

No step of purification happened.
So what we have is a rotten egg supernatant solution with: Amphotericin B, Gentamicin sulfate, fetal bovine serum.
Which is not a "isolate" of a virus.

Then what they did to the animals (note the absense of a control in every step)
>On the day of challenge (Day 0), mice were placed in the nose-only inhalation restraint tubes and connected to the inhalation challenge
>picrel
> All groups of mice (n = 8) were challenged with PR8 aerosol or vehicle (PBS) for 30 min.
Group 2:
>Groups of mice (n = 8) were anesthetized by exposure to 3% isoflurane in the presence of 3% O2 and inoculated with 30 μL of PR8 diluted in PBS, by applying droplets of the suspension to both nares.

No control in the experiment was present.
cont...