/sci/ - Science & Math

PREVIOUS THREAD: >>9554648

WIKI: https://github.com/EyesOpenCure/EyesOpenProject/wiki (barebones work in progress)

>WTF are you doing?
Protein replacement therapy for retinitis pigmentosa type 25 and also other retinal injuries. EYS is a protein that gets secreted by the retina to protect it from damage. It also belongs to a class of proteins that have some really crazy tissue regeneration properties. [1] We are going to grow it in cell cultures and then inject it, a lot like how diabetics get insulin injections.

>What is your plan?
We are going to start by testing in zebrafish. Some fluorescent tagged EYS is going to get injected into their eye and then we will see if it goes to the right place (retinal outer segment). If all goes well, we then modify some zebrafish so that they are missing EYS, and track the visual decline of treated vs. untreated fish. Visual function is measured by wrapping the fish in a wire electrode, showing them flashes of light, and then recording the electrical signals.

>Are you insane?
No, just extremely motivated.


[1] https://www.sciencedaily.com/releases/2017/06/170606095033.htm