/sci/ - Science & Math

>>1207266

Everyone cheats in college, you haven't realized that yet?

>>1207332

>there's no reason any of us should have evolved faster

Sure is retard in here, move along everyone.

>>1188357

Forgot photodiode array picture!

>>1188296

Now that you have ever separate molecule coming out at a different point in time after the time of injection, you will run each of the solutes (solution leaving the column) through a detector. Depending on your budget and your specific detection needs, you will have different detectors on the ends of your column. A frequently used detector is a UV/Vis photodiode array (pictured) or a mass spectrometer which is quite a deal more expensive and specialized, but provides much more specific information.

The UV/Vis photodiode array works by shining light through your eluate (solute) and seeing how each wavelength of light is absorbed by the sample. Since it is a photodiode array, an entire spectrum (lets say 200 to 700 nm) can be taken every time the array is turned on (for example an entire spectrum of data every 0.1 second.)

Basically, when you can get an entire light spectrum of the absorbed wavelengths every second, you can compare the solute that is flowing through at that instant in time through the detector to the wavelengths being absorbed and gain useful knowledge as to the composition of one particular molecule in your unknown solution.

A mass spectrometer basically works by ionizing (breaking) the molecule passing through the detector into smaller pieces and then using electric and magnetic fields to seperate the different sized pieces for counting. For example, if a molecule with a mass to charge (m/z) ratio of 331.232351832 were to be detected by your mass spectrometer you could calculate the exact elemental composition of the molecule flowing through the detector at that point in time, which is VERY useful at determining the overall structure of the molecule.

These are just two of the MANY detectors used on HPLC and GC (gas) instruments, and to be honest, they are quite fun to use.

High Performance Liquid Chromatography (HPLC) is a technique used frequently in chemistry to separate and then analyze components of a particular solution.

The real value of this technique is that you can inject a complex solution with many different molecules into the instrument and separate each component so that each can be analyzed by a specific detector at the end of the process.

Attached is a picture of a fairly standard HPLC instrument. The basic premise of the workings is that you have a sample vial of your unknown solution which is injected in addition to a solvent to a chromatography column which can be filled with tiny (nanometer) particles of different compositions. When the solvent and unknown solution mixture hits the column the unknown solution will instantly begin to partition in the column. When the solution partitions, some of the unknown compounds will remain "stationary" by absorbing or adsorbing onto the column while other compounds transition between phases more quickly and move down the column at a faster rate of speed. What this means in simple terms is that certain molecules will come out of the end of the chromatography column faster than others and depending on column conditions, you can efficiently separate every component of your unknown solution.
Part 1 of ?

>>1188219

I could explain about instruments, but I only have general knowledge in all areas inorg, orgo, instrumentation and techniques.

I highly enjoyed this thread, and if I was not a chemistry major with no interesting knowledge to contribute I would gladly contribute something.

Ive been here since /sci/ began, and it was never good.

>>1171608

Quit being a faggot, you are the reason the only threads we have here are troll threads.

>>1161128

We have quite a number of those filled with nitrogen in our lab, and since our system pulls nitrogen from the air they are constantly venting to prevent overpressure. Scares the shit out of you if you are standing next to one when it starts to violently vent into your face.

I enjoy this thread. I remember helping my prof while he was taking apart our GC with triple quad mass spec and it was wicked complex on the inside. I wish I had taken some pictures.

Hundreds of wieners.

Thank god you have trip codes so I can filter all of you

>>1149942

Any specific book in which he discusses it?

Of course we will. Suck it up, do you think people 100 years ago thought we would be splitting an atom to provide power?

Dont know OP, ill be right there with you though

>>1134015

Same, but then I have to be there at the heat death of the universe ;__;

Hey /sci/

Who else just likes to ponder how huge and awesome the universe is?

Earth is pretty sweet, but I am sad I cant just surf the cosmos and know the near infinite space that our universe offers.

Pic related, my face when I realize how big our observable universe is.

Come on pascal, I thought you were better :(

Agree

Hope you have a fume hood in your house if you want to actually do anything with all of this glassware...

A FUME HOOD

Just go to a local university then and ask if you can be a research assistant for a chemistry professor involving mostly chemistry bitchwork. Then if you worked into it you could use the chemicals in the lab to try to work side reactions.

OP, unless you manage to steal thousands of dollars of glassware and chemicals to synthesize real molecules, what exactly are you going to do with one beaker and a bunsen burner?

Dont want to rain on your parade, but you are most likely going to kill yourself taking your own homebrewed impure aspirin.